pEZ BAC
BAC vector with inducible copy number, suitable for cloning toxic inserts.
Sequence Author: Lucigen
Explore Over 2.7k Plasmids: Lucigen Vectors | More Plasmid Sets
No matches
| ||
Efficient cleavage requires at least two copies of the SacII recognition sequence. |
| ||
FseI gradually loses activity when stored at -20°C. |
| ||
The 1-base overhangs produced by BciVI may be hard to ligate.Sticky ends from different BciVI sites may not be compatible. |
|
| ||
The 1-base overhangs produced by XcmI may be hard to ligate.Sticky ends from different XcmI sites may not be compatible. |
|
| ||
The 1-base overhangs produced by EcoNI may be hard to ligate.Sticky ends from different EcoNI sites may not be compatible. |
| ||
SmaI can be used at 37°C for brief incubations. |
|
| ||
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
|
| ||
BsrGI is typically used at 37°C, but is even more active at 60°C. |
|
| ||
Sticky ends from different BlpI sites may not be compatible. |
|
|
| ||
Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
| ||
Sticky ends from different BstXI sites may not be compatible. |
|
|
| ||
The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible. |
| ||
The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible. |
| ||
Sticky ends from different BseRI sites may not be compatible.BseRI quickly loses activity at 37°C.Prolonged incubation with BseRI may lead to degradation of the DNA. |
| ||
| * Blocked by Dam methylation. BclI is typically used at 50-55°C, but is 50% active at 37°C. |
|
|
| ||
This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C. |
|
|
|
| ||
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
|
|
|
| ||
Efficient cleavage requires at least two copies of the NarI recognition sequence. |
|
| ||
Cleavage may be enhanced when more than one copy of the BbeI recognition sequence is present. |
|
|
| ||
Sticky ends from different EcoO109I sites may not be compatible. |
|