pJAZZ-OC

Linear bacterial vector with a chloramphenicol resistance gene for cloning DNA sequences that are unstable in circular plasmids.

Sequence Author: Lucigen

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HomePlasmidsLucigen VectorspJAZZ-OC
12,500 10,000 7500 5000 2500 End (12,887) PacI (12,391) AanI - AanI (12,044) BglII (11,781) tonB terminator AvrII (11,729) NcoI (11,583) cat promoter rrnB T1 terminator NZ RevC (10,877 .. 10,898) SwaI (10,898) NotI (10,845) AhdI (10,836) MCS-R SmaI (10,821) PvuI (10,769) BsrBI (10,494) lac promoter BmtI (10,366) NheI (10,362) NaeI (10,355) NgoMIV (10,353) SmaI (10,333) ApaI (10,324) PspOMI (10,320) MCS-L AhdI (10,312) NotI (10,300) SpeI (10,293) SL1 (10,272 .. 10,292) T7Te terminator XbaI (10,207) AanI - AanI (9985) AanI - AanI (9948) RsrII (9656) BbvCI (8044) AanI - AanI (7664) BbeI (7282) SfoI (7280) NarI (7279) KasI (7278) XhoI - PspXI - PaeR7I (6350) BsrGI (5170) AanI - AanI (4980) AatII (4824) ZraI (4822) BstBI (4086) SexAI * (3996) MauBI (3125) SacI (1874) Eco53kI (1872) AarI (1785) PpuMI (1632) SgrDI (1402) AanI - AanI (1328) BstZ17I (1064) PmeI (956) AanI - AanI (728) Start (0) telL TelN RepA CB lacZ α CmR telR N15 ori pJAZZ®-OC 12,887 bp
End  (12,887)
0 sites
PacI  (12,391)
1 site		 T T A A T T A A A A T T A A T T
AanI  (12,044)
7 sites		 T T A T A A A A T A T T
AanI  (12,044)
7 sites		 T T A T A A A A T A T T
BglII  (11,781)
1 site		 A G A T C T T C T A G A
AvrII  (11,729)
1 site		 C C T A G G G G A T C C
NcoI  (11,583)
1 site		 C C A T G G G G T A C C
SwaI  (10,898)
1 site		 A T T T A A A T T A A A T T T A
SwaI is typically used at 25°C, but is 50% active at 37°C.
NotI  (10,845)
2 sites		 G C G G C C G C C G C C G G C G
AhdI  (10,836)
2 sites		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
SmaI  (10,821)
2 sites		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
PvuI  (10,769)
1 site		 C G A T C G G C T A G C
BsrBI  (10,494)
1 site		 C C G C T C G G C G A G
This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C.
BmtI  (10,366)
1 site		 G C T A G C C G A T C G
NheI  (10,362)
1 site		 G C T A G C C G A T C G
NaeI  (10,355)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (10,353)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
SmaI  (10,333)
2 sites		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
ApaI  (10,324)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
PspOMI  (10,320)
1 site		 G G G C C C C C C G G G
AhdI  (10,312)
2 sites		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
NotI  (10,300)
2 sites		 G C G G C C G C C G C C G G C G
SpeI  (10,293)
1 site		 A C T A G T T G A T C A
XbaI  (10,207)
1 site		 T C T A G A A G A T C T
AanI  (9985)
7 sites		 T T A T A A A A T A T T
AanI  (9985)
7 sites		 T T A T A A A A T A T T
AanI  (9948)
7 sites		 T T A T A A A A T A T T
AanI  (9948)
7 sites		 T T A T A A A A T A T T
RsrII  (9656)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BbvCI  (8044)
1 site		 C C T C A G C G G A G T C G
AanI  (7664)
7 sites		 T T A T A A A A T A T T
AanI  (7664)
7 sites		 T T A T A A A A T A T T
BbeI  (7282)
1 site		 G G C G C C C C G C G G
Cleavage may be enhanced when more than one copy of the BbeI recognition sequence is present.
SfoI  (7280)
1 site		 G G C G C C C C G C G G
NarI  (7279)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (7278)
1 site		 G G C G C C C C G C G G
XhoI  (6350)
1 site		 C T C G A G G A G C T C
PspXI  (6350)
1 site		 V C T C G A G B B G A G C T C V
PaeR7I  (6350)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
BsrGI  (5170)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
AanI  (4980)
7 sites		 T T A T A A A A T A T T
AanI  (4980)
7 sites		 T T A T A A A A T A T T
AatII  (4824)
1 site		 G A C G T C C T G C A G
ZraI  (4822)
1 site		 G A C G T C C T G C A G
BstBI  (4086)
1 site		 T T C G A A A A G C T T
SexAI  (3996)
1 site		 A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
MauBI  (3125)
1 site		 C G C G C G C G G C G C G C G C
SacI  (1874)
1 site		 G A G C T C C T C G A G
Eco53kI  (1872)
1 site		 G A G C T C C T C G A G
AarI  (1785)
1 site		 C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4
Cleavage may be enhanced when more than one copy of the AarI recognition sequence is present.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.
PpuMI  (1632)
1 site		 R G G W C C Y Y C C W G G R
Sticky ends from different PpuMI sites may not be compatible.
SgrDI  (1402)
1 site		 C G T C G A C G G C A G C T G C
AanI  (1328)
7 sites		 T T A T A A A A T A T T
AanI  (1328)
7 sites		 T T A T A A A A T A T T
BstZ17I  (1064)
1 site		 G T A T A C C A T A T G
PmeI  (956)
1 site		 G T T T A A A C C A A A T T T G
AanI  (728)
7 sites		 T T A T A A A A T A T T