pTargeT (linearized)

Linearized TA cloning vector with 3'-T overhangs for expressing PCR-amplified genes in mammalian cells.

Sequence Author: Promega

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BglII (4382) AlwNI (3961) AhdI (3482) BpmI (3413) ScaI (3001) XmnI (2882) EcoO109I (2502) PfoI (2443) BstXI (2241) BstBI (2202) RsrII (2036) BssHII (1917) PflFI - Tth111I (1638) BbeI (1523) SfoI (1521) NarI (1520) KasI (1519) BsrGI (4483) SpeI (4539) CMV enhancer NdeI (4774) SnaBI (4880) AsiSI (5051) Eco53kI (5114) SacI (5116) BmgBI (5536) lacZα AanI (5615) EcoRI (5637) BamHI (5643) NheI (5651) BmtI (5655) PaeR7I - XhoI (5657) AflIII - MluI (5663) End (5672) Start (1) TspMI - XmaI (8) SmaI (10) Acc65I (14) KpnI (18) SalI (20) AccI (21) NotI (28) EcoRI (35) pTargeT™ Sequencing Primer (61 .. 84) lac operator AanI (361) HpaI (381) MfeI (390) BsaBI * (483) BspDI * - ClaI * (487) BspEI (492) PmlI (566) DraIII (747) AanI (872) SexAI * (1093) SfiI (1279) BseRI (1322) StuI (1325) AvrII (1326) pTargeT™ 5671 bp <