pTrcHis-TOPO (linearized)

Linearized bacterial vector with 3'-T overhangs and bound topoisomerase, for TOPO® TA cloning and inducible expression of N-terminally 6xHis-tagged proteins.

Sequence Author: Thermo Fisher (Invitrogen)

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: TA and GC Cloning Vectors | More Plasmid Sets
No matches
PluTI (3745) SfoI (3743) NarI * (3742) KasI (3741) HpaI (3608) EcoRV (3552) ApaI - BanII (3313) PspOMI (3309) BstEII (3283) BclI * (3116) MluI (3102) PflMI (2684) NsiI (2665) SphI (2663) MauBI (2641) PfoI (2573) PflFI - Tth111I (2474) BsaAI (2468) BstZ17I (2449) AccI (2448) NdeI (2397) BspQI - SapI (2335) PciI (2218) AlwNI (1809) BfuAI - BspMI (3945) lac operator gene 10 translational enhancer RBS minicistron BtgI - NcoI - StyI (4284) ATG 6xHis NheI (4322) BmtI (4326) T7 tag (gene 10 leader) Xpress™ tag BsaBI * (4377) BamHI (4378) End (4390) Start (1) EcoRI (7) BstBI (16) HindIII (19) ScaI (849) PvuI (961) FspI (1107) BglI (1212) AhdI (1330) pTrcHis-TOPO® 4389 bp
PluTI  (3745)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (3743)
1 site
G G C G C C C C G C G G
NarI  (3742)
1 site
G G C G C C C C G C G G
* Blocked by Dcm methylation.
Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (3741)
1 site
G G C G C C C C G C G G
HpaI  (3608)
1 site
G T T A A C C A A T T G
EcoRV  (3552)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
ApaI  (3313)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
BanII  (3313)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
PspOMI  (3309)
1 site
G G G C C C C C C G G G
BstEII  (3283)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BclI  (3116)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
MluI  (3102)
1 site
A C G C G T T G C G C A
PflMI  (2684)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
NsiI  (2665)
1 site
A T G C A T T A C G T A
SphI  (2663)
1 site
G C A T G C C G T A C G
MauBI  (2641)
1 site
C G C G C G C G G C G C G C G C
PfoI  (2573)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
PflFI  (2474)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2474)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BsaAI  (2468)
1 site
Y A C G T R R T G C A Y
BstZ17I  (2449)
1 site
G T A T A C C A T A T G
AccI  (2448)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
NdeI  (2397)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
BspQI  (2335)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (2335)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
PciI  (2218)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
AlwNI  (1809)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BfuAI  (3945)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (3945)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BtgI  (4284)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (4284)
1 site
C C A T G G G G T A C C
StyI  (4284)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
NheI  (4322)
1 site
G C T A G C C G A T C G
BmtI  (4326)
1 site
G C T A G C C G A T C G
BsaBI  (4377)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
BamHI  (4378)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
End  (4390)
0 sites
Start  (1)
0 sites
EcoRI  (7)
1 site
G A A T T C C T T A A G
BstBI  (16)
1 site
T T C G A A A A G C T T
HindIII  (19)
1 site