pLenti6 V5-D-TOPO

Lentiviral directional TOPO® cloning vector for C-terminal tagging of a protein with a V5 epitope tag.

Sequence Author: Thermo Fisher (Invitrogen)

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PciI (4114) AhdI (3226) ScaI (2745) DraIII (2086) NaeI (1980) NgoMIV (1978) SfiI (1598) KpnI (978) Acc65I (974) BlpI (955) KasI (4937) NarI (4938) SfoI (4939) PluTI (4941) AccI (5262) NotI (5444) EcoNI (5466) MfeI (5485) BbvCI - Bpu10I (5720) AleI (5873) KflI (6230) BspDI - ClaI (6319) NdeI (6513) SnaBI (6619) BsmBI - Esp3I (6868) BamHI (6924) SpeI (6930) BstXI (6943) PaeR7I - PspXI - XhoI (6) PspOMI (18) ApaI (22) SacII (25) BstBI (29) MluI (73) AgeI (79) CsiI - SexAI * (198) TspMI - XmaI (452) SmaI (454) BclI * (482) PmlI (491) HincII (496) MscI (563) BsaBI * (858) pLenti6/V5-D-TOPO® 6959 bp
PciI  (4114)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
AhdI  (3226)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
ScaI  (2745)
1 site
A G T A C T T C A T G A
DraIII  (2086)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
NaeI  (1980)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (1978)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
SfiI  (1598)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
KpnI  (978)
1 site
G G T A C C C C A T G G
Acc65I  (974)
1 site
G G T A C C C C A T G G
BlpI  (955)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
KasI  (4937)
1 site
G G C G C C C C G C G G
NarI  (4938)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (4939)
1 site
G G C G C C C C G C G G
PluTI  (4941)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
AccI  (5262)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
NotI  (5444)
1 site
G C G G C C G C C G C C G G C G
EcoNI  (5466)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
MfeI  (5485)
1 site
C A A T T G G T T A A C
BbvCI  (5720)
1 site
C C T C A G C G G A G T C G
Bpu10I  (5720)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
AleI  (5873)
1 site
C A C N N N N G T G G T G N N N N C A C
KflI  (6230)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
BspDI  (6319)
1 site
A T C G A T T A G C T A
ClaI  (6319)
1 site
A T C G A T T A G C T A
NdeI  (6513)
1 site
C A T A T G G T A T A C