pcDNA6.2 C-EmGFP-GW TOPO (linearized)

Linearized vector with 3'-T overhangs and bound topoisomerase, for TOPO® TA cloning to add a C-terminal GFP tag and create a Gateway® expression clone.

Sequence Author: Thermo Fisher (Invitrogen)

Try SnapGene for Free
|Download SnapGene Viewer
Explore Over 2.7k Plasmids: TOPO Cloning Vectors | More Plasmid Sets
No matches
HomePlasmidsTOPO Cloning VectorspcDNA6.2 C-EmGFP-GW TOPO (linearized)
SgrDI (4859) SspI (4742) ScaI (4418) AhdI (3938) PciI (3045) BspQI - SapI (2929) BstZ17I (2666) BsmI (2614) BsaBI * (2276) PpuMI (2137) EagI (2094) BbsI (2063) MscI (1981) BclI * (1900) SmaI (1872) AvaI - BsoBI - TspMI - XmaI (1870) MfeI (5022) MluI (5089) SpeI (5110) NdeI (5345) SnaBI (5451) Eco53kI (5677) SacI (5679) End (5814) Start (1) HpaI (48) PmeI (781) CsiI - SexAI * (1616) StuI (1848) AvrII (1849) pcDNA™6.2/C-EmGFP-GW TOPO® 5813 bp
SgrDI  (4859)
1 site		 C G T C G A C G G C A G C T G C
SspI  (4742)
1 site		 A A T A T T T T A T A A
ScaI  (4418)
1 site		 A G T A C T T C A T G A
AhdI  (3938)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PciI  (3045)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
BspQI  (2929)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (2929)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BstZ17I  (2666)
1 site		 G T A T A C C A T A T G
BsmI  (2614)
1 site		 G A A T G C N C T T A C G N
Sticky ends from different BsmI sites may not be compatible.
BsaBI  (2276)
1 site		 G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
PpuMI  (2137)
1 site		 R G G W C C Y Y C C W G G R
Sticky ends from different PpuMI sites may not be compatible.
EagI  (2094)
1 site		 C G G C C G G C C G G C
BbsI  (2063)
1 site		 G A A G A C N N C T T C T G N N ( N ) 4
Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
MscI  (1981)
1 site		 T G G C C A A C C G G T
BclI  (1900)
1 site		 T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
SmaI  (1872)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
AvaI  (1870)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different AvaI sites may not be compatible.
BsoBI  (1870)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
TspMI  (1870)
1 site		 C C C G G G G G G C C C
XmaI  (1870)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
MfeI  (5022)
1 site		 C A A T T G G T T A A C
MluI  (5089)
1 site		 A C G C G T T G C G C A
SpeI  (5110)
1 site		 A C T A G T T G A T C A
NdeI  (5345)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (5451)
1 site		 T A C G T A A T G C A T
Eco53kI  (5677)
1 site		 G A G C T C C T C G A G
SacI  (5679)
1 site		 G A G C T C C T C G A G
End  (5814)
0 sites
Start  (1)
0 sites
HpaI  (48)
1 site		 G T T A A C C A A T T G
PmeI  (781)
1 site		 G T T T A A A C C A A A T T T G
CsiI  (1616)
1 site		 A C C W G G T T G G W C C A
Sticky ends from different CsiI sites may not be compatible.
SexAI  (1616)
1 site		 A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
StuI  (1848)
1 site		 A G G C C T T C C G G A
AvrII  (1849)
1 site		 C C T A G G G G A T C C
AmpR
3865 .. 4725  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   3865 .. 4656  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3865 .. 4725  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   4657 .. 4725  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3865 .. 4725  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
EmGFP
52 .. 771  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
   Segment 1:  
   52 .. 54  =  3 bp
   1 amino acid  =  149.2 Da
Product: Emerald GFP
mammalian codon-optimized
EmGFP
52 .. 771  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
   Segment 2:  1a  
   55 .. 57  =  3 bp
   1 amino acid  =  117.1 Da
Product: Emerald GFP
mammalian codon-optimized
EmGFP
52 .. 771  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
   Segment 3:  
   58 .. 771  =  714 bp
   237 amino acids  =  26.7 kDa
Product: Emerald GFP
mammalian codon-optimized
EmGFP
52 .. 771  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
Product: Emerald GFP
mammalian codon-optimized
ori
3106 .. 3694  =  589 bp
high-copy-number colE1/pMB1/pBR322/pUC origin of replication
ori
3106 .. 3694  =  589 bp
high-copy-number colE1/pMB1/pBR322/pUC origin of replication
f1 ori
1093 .. 1521  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
1093 .. 1521  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
BSD
1978 .. 2376  =  399 bp
132 amino acids  =  13.7 kDa
Product: blasticidin S deaminase
confers resistance to blasticidin
BSD
1978 .. 2376  =  399 bp
132 amino acids  =  13.7 kDa
Product: blasticidin S deaminase
confers resistance to blasticidin
CMV enhancer
5096 .. 5475  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
5096 .. 5475  =  380 bp
human cytomegalovirus immediate early enhancer
SV40 promoter
1535 .. 1864  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
1535 .. 1864  =  330 bp
SV40 enhancer and early promoter
CMV promoter
5476 .. 5679  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
5476 .. 5679  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal
2534 .. 2655  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
2534 .. 2655  =  122 bp
SV40 polyadenylation signal
AmpR promoter
4726 .. 4830  =  105 bp
AmpR promoter
4726 .. 4830  =  105 bp
HSV TK poly(A) signal
843 .. 891  =  49 bp
herpesvirus thymidine kinase polyadenylation signal
HSV TK poly(A) signal
843 .. 891  =  49 bp
herpesvirus thymidine kinase polyadenylation signal
EM7 promoter
1912 .. 1959  =  48 bp
synthetic bacterial promoter
EM7 promoter
1912 .. 1959  =  48 bp
synthetic bacterial promoter
attB2
14 .. 38  =  25 bp
recombination site for the Gateway® BP reaction
attB2
14 .. 38  =  25 bp
recombination site for the Gateway® BP reaction
attB1
5776 .. 5800  =  25 bp
recombination site for the Gateway® BP reaction
attB1
5776 .. 5800  =  25 bp
recombination site for the Gateway® BP reaction
T7 promoter
5724 .. 5742  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
5724 .. 5742  =  19 bp
promoter for bacteriophage T7 RNA polymerase
SV40 ori
1715 .. 1850  =  136 bp
SV40 origin of replication
SV40 ori
1715 .. 1850  =  136 bp
SV40 origin of replication
ORF:  1 .. 771  =  771 bp
ORF:  256 amino acids  =  28.8 kDa  (no start codon)
ORF:  1978 .. 2376  =  399 bp
ORF:  132 amino acids  =  13.7 kDa
ORF:  3995 .. 4261  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  1924 .. 2280  =  357 bp
ORF:  118 amino acids  =  12.8 kDa
ORF:  3865 .. 4725  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
Click here to try SnapGene

Download pcDNA6.2 C-EmGFP-GW TOPO (linearized).dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure
Download SnapGene

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps
Download SnapGene Viewer

Individual Sequences & Maps

pBAD-TOPO (linearized)
pcDNA5 FRT TO-TOPO (linearized)
pEF6 V5-His-TOPO (linearized)
pGlow-TOPO (linearized)
pBAD Thio-TOPO (linearized)
pcDNA5 FRT V5-His-TOPO (linearized)
pENTR D-TOPO
pIB V5-His-TOPO (linearized)
pBAD202 D-TOPO
pcDNA6.2 C-EmGFP-GW TOPO (linearized)
pENTR SD D-TOPO
pLenti6.3 V5-TOPO (linearized)
pBlue-TOPO (linearized)
pcDNA6.2 C-YFP-GW TOPO (linearized)
pENTR TEV D-TOPO
pLenti6 V5-D-TOPO
pcDNA3.1D V5-His-TOPO
pcDNA6.2 N-EmGFP-GW TOPO (linearized)
pENTR5'-TOPO (linearized)
pLenti7.3 V5-TOPO (linearized)
pcDNA3.2 V5 GW D-TOPO
pCR-XL-2-TOPO (linearized)
pET100 D-TOPO
pLPS-B (linearized)
pcDNA6.2 cGeneBLAzer-GW D-TOPO
pCR-XL-TOPO (linearized)
pET101 D-TOPO
pMT V5-His-TOPO (linearized)
pcDNA6.2 V5 GW D-TOPO
pCR2.1-TOPO (linearized)
pET102 D-TOPO
pOptiVEC-TOPO (linearized)
pcDNA3.1 CT-GFP-TOPO (linearized)
pCR4-TOPO (linearized)
pET151 D-TOPO
pSecTag FRT V5-His-TOPO (linearized)
pcDNA3.1 NT-GFP-TOPO (linearized)
pCR4Blunt-TOPO (linearized)
pET160 GW D-TOPO
pTOP Blunt V2 (linearized)
pcDNA3.1 V5-His-TOPO (linearized)
pCR8 GW TOPO (linearized)
pET161 GW D-TOPO
pTrcHis-TOPO (linearized)
pcDNA3.3-TOPO (linearized)
pCR-Blunt II-TOPO (linearized)
pET200 D-TOPO
pTrcHis2-TOPO (linearized)
pcDNA3.4-TOPO (linearized)
pCRII-TOPO (linearized)
pEXP5-CT TOPO (linearized)
pYES2.1 V5-His-TOPO (linearized)
pcDNA4 HisMax-TOPO (linearized)
pEF5 FRT V5-D-TOPO
pEXP5-NT TOPO (linearized)

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.
Try for Free