MERS Genome

Complete genome of the Middle East respiratory syndrome (MERS) coronavirus.
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HomePlasmidsCoronavirus ResourcesMERS Genome
30,000 20,000 10,000 End (30,119) SmaI (30,030) XmaI - TspMI (30,028) SacII (29,156) M E KflI (27,534) PacI (25,840) PspXI (25,488) PvuI - BsiEI (18,775) BstEII (10,076) BbvCI (3118) BamHI (806) BssHII (312) Start (0) orf1ab orf3 orf4b orf5 N orf1ab mature peptide mature peptide S orf4a orf8b mature peptide mature peptide mature peptide mature peptide mature peptide mature peptide mature peptide mature peptide mature peptide mature peptide mature peptide mature peptide mature peptide MERS Genome 30,119 bp
End  (30,119)
0 sites
SmaI  (30,030)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
XmaI  (30,028)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
TspMI  (30,028)
1 site		 C C C G G G G G G C C C
SacII  (29,156)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
KflI  (27,534)
1 site		 G G G W C C C C C C W G G G
Sticky ends from different KflI sites may not be compatible.
PacI  (25,840)
1 site		 T T A A T T A A A A T T A A T T
PspXI  (25,488)
1 site		 V C T C G A G B B G A G C T C V
PvuI  (18,775)
1 site		 C G A T C G G C T A G C
BsiEI  (18,775)
1 site		 C G R Y C G G C Y R G C
Sticky ends from different BsiEI sites may not be compatible.
BstEII  (10,076)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BbvCI  (3118)
1 site		 C C T C A G C G G A G T C G
BamHI  (806)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BssHII  (312)
1 site		 G C G C G C C G C G C G
BssHII is typically used at 50°C, but is 75% active at 37°C.
Start  (0)
0 sites
orf1ab
279 .. 21,514  =  21,236 bp
7078 amino acids  =  789.5 kDa
2 segments
   Segment 1:  
   279 .. 13,433  =  13,155 bp
   4385 amino acids  =  485.4 kDa
Product: 1AB polyprotein
ORF1ab product; pp1ab
orf1ab
279 .. 21,514  =  21,236 bp
7078 amino acids  =  789.5 kDa
2 segments
   Segment 2:  
   13,433 .. 21,514  =  8082 bp
   2693 amino acids  =  304.1 kDa
Product: 1AB polyprotein
ORF1ab product; pp1ab
orf1ab
279 .. 21,514  =  21,236 bp
7078 amino acids  =  789.5 kDa
2 segments
Product: 1AB polyprotein
ORF1ab product; pp1ab
orf3
25,532 .. 25,843  =  312 bp
103 amino acids  =  11.1 kDa
Product: NS3 protein
ORF3 protein
orf3
25,532 .. 25,843  =  312 bp
103 amino acids  =  11.1 kDa
Product: NS3 protein
ORF3 protein
orf4b
26,093 .. 26,833  =  741 bp
246 amino acids  =  28.6 kDa
Product: NS4B protein
ORF4b protein
orf4b
26,093 .. 26,833  =  741 bp
246 amino acids  =  28.6 kDa
Product: NS4B protein
ORF4b protein
orf5
26,840 .. 27,514  =  675 bp
224 amino acids  =  25.3 kDa
Product: NS5 protein
ORF5 protein
orf5
26,840 .. 27,514  =  675 bp
224 amino acids  =  25.3 kDa
Product: NS5 protein
ORF5 protein
E
27,590 .. 27,838  =  249 bp
82 amino acids  =  9.4 kDa
Product: envelope protein
E protein
E
27,590 .. 27,838  =  249 bp
82 amino acids  =  9.4 kDa
Product: envelope protein
E protein
M
27,853 .. 28,512  =  660 bp
219 amino acids  =  24.5 kDa
Product: membrane protein
M protein
M
27,853 .. 28,512  =  660 bp
219 amino acids  =  24.5 kDa
Product: membrane protein
M protein
N
28,566 .. 29,807  =  1242 bp
413 amino acids  =  45.1 kDa
Product: nucleoprotein
N protein
N
28,566 .. 29,807  =  1242 bp
413 amino acids  =  45.1 kDa
Product: nucleoprotein
N protein
orf1ab
279 .. 13,454  =  13,176 bp
4391 amino acids  =  486.0 kDa
Product: 1A polyprotein
ORF1a product; pp1a
orf1ab
279 .. 13,454  =  13,176 bp
4391 amino acids  =  486.0 kDa
Product: 1A polyprotein
ORF1a product; pp1a
mature peptide
16,208 .. 18,001  =  1794 bp
Product: nsp13 protein
putative zinc-binding domain, putative superfamily 1 helicase
/protein_id=YP_009047224.1
mature peptide
16,208 .. 18,001  =  1794 bp
Product: nsp13 protein
putative zinc-binding domain, putative superfamily 1 helicase
/protein_id=YP_009047224.1
mature peptide
18,002 .. 19,573  =  1572 bp
Product: nsp14 protein
putative 3' to 5' exonuclease, N7-methyltransferase
/protein_id=YP_009047225.1
mature peptide
18,002 .. 19,573  =  1572 bp
Product: nsp14 protein
putative 3' to 5' exonuclease, N7-methyltransferase
/protein_id=YP_009047225.1
mature peptide
19,574 .. 20,602  =  1029 bp
Product: nsp15 protein
putative nidoviral endoribonuclease specific for U
/protein_id=YP_009047226.1
mature peptide
19,574 .. 20,602  =  1029 bp
Product: nsp15 protein
putative nidoviral endoribonuclease specific for U
/protein_id=YP_009047226.1
S
21,456 .. 25,517  =  4062 bp
1353 amino acids  =  149.4 kDa
Product: spike glycoprotein
S protein
S
21,456 .. 25,517  =  4062 bp
1353 amino acids  =  149.4 kDa
Product: spike glycoprotein
S protein
orf4a
25,852 .. 26,181  =  330 bp
109 amino acids  =  12.2 kDa
Product: NS4A protein
ORF4a protein
orf4a
25,852 .. 26,181  =  330 bp
109 amino acids  =  12.2 kDa
Product: NS4A protein
ORF4a protein
orf8b
28,762 .. 29,100  =  339 bp
112 amino acids  =  12.3 kDa
Product: ORF8b protein
orf8b
28,762 .. 29,100  =  339 bp
112 amino acids  =  12.3 kDa
Product: ORF8b protein
mature peptide
279 .. 857  =  579 bp
Product: nsp1 protein
/protein_id=YP_009047213.1
mature peptide
279 .. 857  =  579 bp
Product: nsp1 protein
/protein_id=YP_009047213.1
mature peptide
858 .. 2837  =  1980 bp
Product: nsp2 protein
/protein_id=YP_009047230.1
mature peptide
858 .. 2837  =  1980 bp
Product: nsp2 protein
/protein_id=YP_009047230.1
mature peptide
2838 .. 8498  =  5661 bp
Product: nsp3 protein
putative ADP-ribose 1'-phosphatase, putative papain-like proteinase 2, putative transmembrane domain 1
/protein_id=YP_009047215.1
mature peptide
2838 .. 8498  =  5661 bp
Product: nsp3 protein
putative ADP-ribose 1'-phosphatase, putative papain-like proteinase 2, putative transmembrane domain 1
/protein_id=YP_009047215.1
mature peptide
8499 .. 10,019  =  1521 bp
Product: nsp4 protein
putative transmembrane domain 2
/protein_id=YP_009047216.1
mature peptide
8499 .. 10,019  =  1521 bp
Product: nsp4 protein
putative transmembrane domain 2
/protein_id=YP_009047216.1
mature peptide
10,020 .. 10,937  =  918 bp
Product: nsp5 protein
putative 3C-like cysteine proteinase
/protein_id=YP_009047233.1
mature peptide
10,020 .. 10,937  =  918 bp
Product: nsp5 protein
putative 3C-like cysteine proteinase
/protein_id=YP_009047233.1
mature peptide
10,938 .. 11,813  =  876 bp
Product: nsp6 protein
putative transmembrane protein 3
/protein_id=YP_009047218.1
mature peptide
10,938 .. 11,813  =  876 bp
Product: nsp6 protein
putative transmembrane protein 3
/protein_id=YP_009047218.1
mature peptide
11,814 .. 12,062  =  249 bp
Product: nsp7 protein
/protein_id=YP_009047235.1
mature peptide
11,814 .. 12,062  =  249 bp
Product: nsp7 protein
/protein_id=YP_009047235.1
mature peptide
12,063 .. 12,659  =  597 bp
Product: nsp8 protein
putative primase
/protein_id=YP_009047236.1
mature peptide
12,063 .. 12,659  =  597 bp
Product: nsp8 protein
putative primase
/protein_id=YP_009047236.1
mature peptide
12,660 .. 12,989  =  330 bp
Product: nsp9 protein
/protein_id=YP_009047237.1
mature peptide
12,660 .. 12,989  =  330 bp
Product: nsp9 protein
/protein_id=YP_009047237.1
mature peptide
12,990 .. 13,409  =  420 bp
Product: nsp10 protein
/protein_id=YP_009047238.1
mature peptide
12,990 .. 13,409  =  420 bp
Product: nsp10 protein
/protein_id=YP_009047238.1
mature peptide
13,410 .. 16,207  =  2798 bp
2 segments
   Segment 1:  
   13,410 .. 13,433  =  24 bp
Product: nsp12 protein
putative RNA-dependent RNA polymerase
/protein_id=YP_009047223.1
mature peptide
13,410 .. 16,207  =  2798 bp
2 segments
   Segment 2:  
   13,433 .. 16,207  =  2775 bp
Product: nsp12 protein
putative RNA-dependent RNA polymerase
/protein_id=YP_009047223.1
mature peptide
13,410 .. 16,207  =  2798 bp
2 segments
Product: nsp12 protein
putative RNA-dependent RNA polymerase
/protein_id=YP_009047223.1
mature peptide
20,603 .. 21,511  =  909 bp
Product: nsp16 protein
putative S-adenosylmethionine-dependent ribose 2'-O-methiltransferase
/protein_id=YP_009047227.1
mature peptide
20,603 .. 21,511  =  909 bp
Product: nsp16 protein
putative S-adenosylmethionine-dependent ribose 2'-O-methiltransferase
/protein_id=YP_009047227.1
mature peptide
279 .. 857  =  579 bp
Product: nsp1 protein
/protein_id=YP_009047229.1
mature peptide
279 .. 857  =  579 bp
Product: nsp1 protein
/protein_id=YP_009047229.1
mature peptide
858 .. 2837  =  1980 bp
Product: nsp2 protein
/protein_id=YP_009047214.1
mature peptide
858 .. 2837  =  1980 bp
Product: nsp2 protein
/protein_id=YP_009047214.1
mature peptide
2838 .. 8498  =  5661 bp
Product: nsp3 protein
putative ADP-ribose 1'-phosphatase, putative papain-like proteinase 2, putative transmembrane domain 1
/protein_id=YP_009047231.1
mature peptide
2838 .. 8498  =  5661 bp
Product: nsp3 protein
putative ADP-ribose 1'-phosphatase, putative papain-like proteinase 2, putative transmembrane domain 1
/protein_id=YP_009047231.1
mature peptide
8499 .. 10,019  =  1521 bp
Product: nsp4 protein
putative transmembrane domain 2
/protein_id=YP_009047232.1
mature peptide
8499 .. 10,019  =  1521 bp
Product: nsp4 protein
putative transmembrane domain 2
/protein_id=YP_009047232.1
mature peptide
10,020 .. 10,937  =  918 bp
Product: nsp5 protein
putative 3C-like cysteine proteinase
/protein_id=YP_009047217.1
mature peptide
10,020 .. 10,937  =  918 bp
Product: nsp5 protein
putative 3C-like cysteine proteinase
/protein_id=YP_009047217.1
mature peptide
10,938 .. 11,813  =  876 bp
Product: nsp6 protein
putative transmembrane domain 3
/protein_id=YP_009047234.1
mature peptide
10,938 .. 11,813  =  876 bp
Product: nsp6 protein
putative transmembrane domain 3
/protein_id=YP_009047234.1
mature peptide
11,814 .. 12,062  =  249 bp
Product: nsp7 protein
/protein_id=YP_009047219.1
mature peptide
11,814 .. 12,062  =  249 bp
Product: nsp7 protein
/protein_id=YP_009047219.1
mature peptide
12,063 .. 12,659  =  597 bp
Product: nsp8 protein
putative primase
/protein_id=YP_009047220.1
mature peptide
12,063 .. 12,659  =  597 bp
Product: nsp8 protein
putative primase
/protein_id=YP_009047220.1
mature peptide
12,660 .. 12,989  =  330 bp
Product: nsp9 protein
/protein_id=YP_009047221.1
mature peptide
12,660 .. 12,989  =  330 bp
Product: nsp9 protein
/protein_id=YP_009047221.1
mature peptide
12,990 .. 13,409  =  420 bp
Product: nsp10 protein
/protein_id=YP_009047222.1
mature peptide
12,990 .. 13,409  =  420 bp
Product: nsp10 protein
/protein_id=YP_009047222.1
mature peptide
13,410 .. 13,451  =  42 bp
Product: nsp11 protein
/protein_id=YP_009047228.1
mature peptide
13,410 .. 13,451  =  42 bp
Product: nsp11 protein
/protein_id=YP_009047228.1
ORF:  490 .. 849  =  360 bp
ORF:  119 amino acids  =  13.2 kDa
ORF:  7390 .. 7758  =  369 bp
ORF:  122 amino acids  =  13.6 kDa
ORF:  22,480 .. 22,716  =  237 bp
ORF:  78 amino acids  =  9.3 kDa
ORF:  25,852 .. 26,181  =  330 bp
ORF:  109 amino acids  =  12.2 kDa
ORF:  27,853 .. 28,512  =  660 bp
ORF:  219 amino acids  =  24.5 kDa
ORF:  28,762 .. 29,100  =  339 bp
ORF:  112 amino acids  =  12.3 kDa
ORF:  29,521 .. 29,787  =  267 bp
ORF:  88 amino acids  =  9.9 kDa
ORF:  13,643 .. 21,514  =  7872 bp
ORF:  2623 amino acids  =  296.2 kDa
ORF:  25,532 .. 25,843  =  312 bp
ORF:  103 amino acids  =  11.1 kDa
ORF:  26,093 .. 26,833  =  741 bp
ORF:  246 amino acids  =  28.6 kDa
ORF:  26,840 .. 27,514  =  675 bp
ORF:  224 amino acids  =  25.3 kDa
ORF:  27,590 .. 27,838  =  249 bp
ORF:  82 amino acids  =  9.4 kDa
ORF:  279 .. 13,454  =  13,176 bp
ORF:  4391 amino acids  =  486.0 kDa
ORF:  15,408 .. 15,677  =  270 bp
ORF:  89 amino acids  =  10.1 kDa
ORF:  21,456 .. 25,517  =  4062 bp
ORF:  1353 amino acids  =  149.4 kDa
ORF:  26,112 .. 26,384  =  273 bp
ORF:  90 amino acids  =  10.1 kDa
ORF:  26,451 .. 26,750  =  300 bp
ORF:  99 amino acids  =  11.4 kDa
ORF:  28,566 .. 29,807  =  1242 bp
ORF:  413 amino acids  =  45.1 kDa
ORF:  2592 .. 2924  =  333 bp
ORF:  110 amino acids  =  12.8 kDa
ORF:  6981 .. 7223  =  243 bp
ORF:  80 amino acids  =  9.0 kDa
ORF:  8598 .. 9080  =  483 bp
ORF:  160 amino acids  =  18.5 kDa
ORF:  9366 .. 9605  =  240 bp
ORF:  79 amino acids  =  9.3 kDa
ORF:  10,038 .. 10,268  =  231 bp
ORF:  76 amino acids  =  8.5 kDa
ORF:  21,489 .. 21,728  =  240 bp
ORF:  79 amino acids  =  9.3 kDa
ORF:  28,632 .. 28,937  =  306 bp
ORF:  101 amino acids  =  11.3 kDa
ORF:  29,229 .. 29,531  =  303 bp
ORF:  100 amino acids  =  11.3 kDa
ORF:  15,413 .. 15,841  =  429 bp
ORF:  142 amino acids  =  16.0 kDa
ORF:  16,697 .. 17,002  =  306 bp
ORF:  101 amino acids  =  11.9 kDa
ORF:  5176 .. 5517  =  342 bp
ORF:  113 amino acids  =  13.2 kDa
ORF:  20,689 .. 20,994  =  306 bp
ORF:  101 amino acids  =  11.1 kDa
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Individual Sequences & Maps

MERS Genome
Primers & Probes for SARS-CoV-2 (COVID-19) Genome
SARS Genome
SARS-CoV-2 (COVID-19) Genome

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
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Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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