T-Vector pMD20

Linearized vector with a pUC19-derived MCS and with a single 3'-T at each end, for TA cloning of PCR products and blue-white screening.

Sequence Author: TaKaRa

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: TA and GC Cloning Vectors | More Plasmid Sets
No matches
HindIII (2687) BspQI - SapI (2418) AflIII - PciI (2301) PspFI (2001) BseYI (1997) AlwNI (1892) AhdI (1413) BsaI (1347) BpmI (1344) BsrFI (1328) NmeAIII (1266) BfuAI - BspMI (2692) SphI (2697) PstI - SbfI (2703) SalI (2705) AccI (2706) HincII (2707) XbaI (2711) SpeI (2723) NdeI (2730) End (2738) Start (1) BamHI (5) AvaI - BsoBI - TspMI - XmaI (10) SmaI (12) Acc65I (14) KpnI (18) Eco53kI (22) BanII - SacI (24) ApoI - EcoRI (26) BstAPI (243) PfoI (374) EcoO109I (433) ZraI (492) AatII (494) SspI (608) XmnI (813) ScaI (932) TsoI (1015) T-Vector pMD20 2737 bp
HindIII  (2687)
1 site
A A G C T T T T C G A A
BspQI  (2418)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (2418)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
AflIII  (2301)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (2301)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
PspFI  (2001)
1 site
C C C A G C G G G T C G
BseYI  (1997)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
AlwNI  (1892)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
AhdI  (1413)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BsaI  (1347)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BpmI  (1344)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BsrFI  (1328)
1 site
R C C G G Y Y G G C C R

Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
NmeAIII  (1266)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BfuAI  (2692)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (2692)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
SphI  (2697)
1 site
G C A T G C C G T A C G
PstI  (2703)
1 site
C T G C A G G A C G T C
SbfI  (2703)
1 site
C C T G C A G G G G A C G T C C
SalI  (2705)
1 site
G T C G A C C A G C T G
AccI  (2706)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
HincII  (2707)
1 site
G T Y R A C C A R Y T G
XbaI  (2711)
1 site
T C T A G A A G A T C T
SpeI  (2723)
1 site
A C T A G T T G A T C A
NdeI  (2730)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
End  (2738)
0 sites
Start  (1)
0 sites
BamHI  (5)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
AvaI  (10)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (10)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
TspMI  (10)
1 site
C C C G G G G G G C C C
XmaI  (10)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (12)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
Acc65I  (14)
1 site
G G T A C C C C A T G G
KpnI  (18)
1 site
G G T A C C C C A T G G
Eco53kI  (22)
1 site
G A G C T C C T C G A G
BanII  (24)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
SacI  (24)
1 site
G A G C T C C T C G A G
ApoI  (26)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
EcoRI  (26)
1 site
G A A T T C C T T A A G
BstAPI  (243)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
PfoI  (374)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
EcoO109I  (433)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
ZraI  (492)
1 site
G A C G T C C T G C A G
AatII  (494)
1 site
G A C G T C C T G C A G
SspI  (608)
1 site
A A T A T T T T A T A A
XmnI  (813)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (932)
1 site
A G T A C T T C A T G A
TsoI  (1015)
1 site
T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9

Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
AmpR
626 .. 1486  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   626 .. 694  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
626 .. 1486  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   695 .. 1486  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
626 .. 1486  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
1657 .. 2245  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1657 .. 2245  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
lacZα
2 .. 194  =  193 bp
63 amino acids  =  7.2 kDa
Product: LacZα fragment of β-galactosidase
lacZα
2 .. 194  =  193 bp
63 amino acids  =  7.2 kDa
Product: LacZα fragment of β-galactosidase
AmpR promoter
521 .. 625  =  105 bp
AmpR promoter
521 .. 625  =  105 bp
lacZα
2643 .. 2736  =  94 bp
31 amino acids  =  3.5 kDa
Product: LacZα fragment of β-galactosidase
lacZα
2643 .. 2736  =  94 bp
31 amino acids  =  3.5 kDa
Product: LacZα fragment of β-galactosidase
lac promoter
2569 .. 2599  =  31 bp
3 segments
   Segment 1:  -35  
   2569 .. 2574  =  6 bp
promoter for the E. coli lac operon
lac promoter
2569 .. 2599  =  31 bp
3 segments
   Segment 2:  
   2575 .. 2592  =  18 bp
promoter for the E. coli lac operon
lac promoter
2569 .. 2599  =  31 bp
3 segments
   Segment 3:  -10  
   2593 .. 2599  =  7 bp
promoter for the E. coli lac operon
lac promoter
2569 .. 2599  =  31 bp
3 segments
promoter for the E. coli lac operon
lac operator
2607 .. 2623  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
2607 .. 2623  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
MCS
2687 .. 2736  =  50 bp
multiple cloning site
MCS
2687 .. 2736  =  50 bp
multiple cloning site
MCS
2 .. 31  =  30 bp
multiple cloning site
MCS
2 .. 31  =  30 bp
multiple cloning site
SP6 promoter
2665 .. 2682  =  18 bp
promoter for bacteriophage SP6 RNA polymerase
SP6 promoter
2665 .. 2682  =  18 bp
promoter for bacteriophage SP6 RNA polymerase
M13 fwd
32 .. 48  =  17 bp
common sequencing primer, one of multiple similar variants
M13 fwd
32 .. 48  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
2631 .. 2647  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
2631 .. 2647  =  17 bp
common sequencing primer, one of multiple similar variants
ORF:  626 .. 1486  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  1090 .. 1356  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
Click here to try SnapGene

Download T-Vector pMD20.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.