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TOPO Cloning Vectors
Commonly used TOPO sequences including blunt, directional, and TOPO TA cloning vectors
Updated December 16th, 2019
These combined DNA sequence and map files can be opened with 
SnapGene or the free 
SnapGene Viewer.
TOPO Cloning Vectors.zip (1 MB)
55 Sequences
55 Sequences
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TOPO Cloning Vectors
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Individual Sequences & Maps
- pBAD-TOPO (linearized)
- pBAD202/D-TOPO
- pBAD/Thio-TOPO (linearized)
- pBlue-TOPO (linearized)
- pcDNA3.1D/V5-His-TOPO
- pcDNA3.1/CT-GFP-TOPO (linearized)
- pcDNA3.1/NT-GFP-TOPO (linearized)
- pcDNA3.1/V5-His-TOPO (linearized)
- pcDNA3.2/V5/GW/D-TOPO
- pcDNA3.3-TOPO (linearized)
- pcDNA3.4-TOPO (linearized)
- pcDNA4/HisMax-TOPO (linearized)
- pcDNA5/FRT/TO-TOPO (linearized)
- pcDNA5/FRT/V5-His-TOPO (linearized)
- pcDNA6.2/C-EmGFP-GW TOPO (linearized)
- pcDNA6.2/C-YFP-GW TOPO (linearized)
- pcDNA6.2/cGeneBLAzer-GW/D-TOPO
- pcDNA6.2/N-EmGFP-GW TOPO (linearized)
- pcDNA6.2/V5/GW/D-TOPO
- pCR-Blunt II-TOPO (linearized)
- pCR-XL-2-TOPO (linearized)
- pCR-XL-TOPO (linearized)
- pCR2.1-TOPO (linearized)
- pCR4-TOPO (linearized)
- pCR4Blunt-TOPO (linearized)
- pCR8/GW/TOPO (linearized)
- pCRII-TOPO (linearized)
- pEF5/FRT/V5-D-TOPO
- pEF6/V5-His-TOPO (linearized)
- pENTR5'-TOPO (linearized)
- pENTR/D-TOPO
- pENTR/SD/D-TOPO
- pENTR/TEV/D-TOPO
- pET100/D-TOPO
- pET101/D-TOPO
- pET102/D-TOPO
- pET151/D-TOPO
- pET160/GW/D-TOPO
- pET161/GW/D-TOPO
- pET200/D-TOPO
- pEXP5-CT/TOPO (linearized)
- pEXP5-NT/TOPO (linearized)
- pGlow-TOPO (linearized)
- pIB/V5-His-TOPO (linearized)
- pLenti6.3/V5-TOPO (linearized)
- pLenti6/V5-D-TOPO
- pLenti7.3/V5-TOPO (linearized)
- pLPS-B (linearized)
- pMT/V5-His-TOPO (linearized)
- pOptiVEC-TOPO (linearized)
- pSecTag/FRT/V5-His-TOPO (linearized)
- pTOP Blunt V2 (linearized)
- pTrcHis-TOPO (linearized)
- pTrcHis2-TOPO (linearized)
- pYES2.1/V5-His-TOPO (linearized)
The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.
