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Simulate TA and GC Cloning

Learn how SnapGene can simulate TA cloning or Lucigen’s GC cloning.

TA and GC Cloning

TA cloning is used to clone PCR products. It takes advantage of the 3′ A overhangs added during amplification by Taq and some other polymerases. These sticky ends enable insertion into a linearized vector with complementary 3′ T overhangs. GC cloning is similar, and is based on the finding that Taq actually adds a mixture of 3′ A and 3′ G overhangs.

SnapGene provides a simple interface for simulating TA and GC cloning. A variety of commercially available linearized TA and GC cloning vectors are embedded in the software.

A list of annotated TA and GC cloning vectors is available online.