Simulate Cloning in SnapGene

Watch these step-by-step video guides and learn how to simulate all major molecular cloning techniques in SnapGene.

Restriction Enzyme Cloning in SnapGene

INSIDE THE VIDEO
Using a specific example, you will learn to simulate inserting a single fragment into a plasmid vector using restriction and insertion cloning in SnapGene.
LENGTH
4 mins 50 mins

TA and GC Cloning in SnapGene

INSIDE THE VIDEO
TA and GC cloning are two simple techniques that do not require restriction enzymes. This video will show you how to simulate these cloning techniques in SnapGene.
LENGTH
5 minutes

TOPO Cloning with SnapGene

INSIDE THE VIDEO
Learn how to simulate TOPO cloning in SnapGene with this step-by-step tutorial.
LENGTH
5 minutes

Gateway Cloning with SnapGene

INSIDE THE VIDEO
Gateway cloning does not require restriction enzymes to insert DNA into a plasmid. Instead, Gateway Cloning uses a two-step recombination process. This video talks you through the steps needed to simulate Gateway Cloning in SnapGene.
LENGTH
5 minutes

Gateway Cloning with Multiple Inserts

INSIDE THE VIDEO
Learn how to simulate gateway cloning with multiple inserts in SnapGene.
LENGTH
4 minutes

Golden Gate Assembly in SnapGene

INSIDE THE VIDEO
Learn how to simulate Golden Gate Assembly, also known as Golden Gate cloning, in SnapGene.
LENGTH
4 minutes

In-Fusion Cloning with SnapGene

INSIDE THE VIDEO
This video will show you how to simulate Takara's In-Fusion Cloning with a single fragment in SnapGene. In-Fusion Cloning is a ligation-independent cloning technique that uses complementary annealing of an insert and a vector.
LENGTH
5 minutes

In-Fusion Cloning with Multiple Inserts

INSIDE THE VIDEO
Learn how to simulate in-fusion cloning with multiple inserts by following this step-by-step tutorial.
LENGTH
4 minutes

Gibson Assembly with SnapGene

INSIDE THE VIDEO
A step-by-step guide on simulating a simple Gibson Assembly cloning experiment in SnapGene.
LENGTH
5 minutes

Gibson Assembly with Multiple Inserts

INSIDE THE VIDEO
Watch this tutorial video and learn how to simulate multi-insert Gibson assembly in SnapGene.
LENGTH
4 minutes

NEBuilder HiFi Assembly in SnapGene

INSIDE THE VIDEO
This step-by-step tutorial shows you how to simulate NEBuilder HiFi Assembly in SnapGene.
LENGTH
3 minutes

NEBuilder HiFi Assembly with Multiple Inserts

INSIDE THE VIDEO
Follow this step-by-step tutorial and learn how to use SnapGene to simulate NEBuilder HiFi Assembly with multiple inserts.
LENGTH
4 minutes

Addgene - Annealed Oligo Cloning

INSIDE THE VIDEO
Watch this video to learn how to add new restriction sites to the MCS of an empty vector with oligo overlap cloning.
LENGTH
8 minutes
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