pGAPZ(alpha) B
Pichia pastoris vector for constitutive expression of a secreted protein. For other reading frames, use pGAPZα A or pGAPZα C.
Sequence Author: Thermo Fisher (Invitrogen)
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Sticky ends from different AlwNI sites may not be compatible. |
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The 1-base overhangs produced by BciVI may be hard to ligate.Sticky ends from different BciVI sites may not be compatible. |
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PciI is inhibited by nonionic detergents. |
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BsrGI is typically used at 37°C, but is even more active at 60°C. |
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EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
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| * Blocked by Dcm methylation. |
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Sticky ends from different KflI sites may not be compatible. |
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Sticky ends from different DraIII sites may not be compatible. |
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FseI gradually loses activity when stored at -20°C. |
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Sticky ends from different CsiI sites may not be compatible. |
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| * Blocked by Dcm methylation. Sticky ends from different SexAI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the SgrAI recognition sequence. |
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SmaI can be used at 37°C for brief incubations. |
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Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
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BssHII is typically used at 50°C, but is 75% active at 37°C. |
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Sticky ends from different TsoI sites may not be compatible.After cleavage, TsoI can remain bound to DNA and alter its electrophoretic mobility.For full activity, add fresh S-adenosylmethionine (SAM). |
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Efficient cleavage requires at least two copies of the BfuAI recognition sequence. Sticky ends from different BfuAI sites may not be compatible.BfuAI is typically used at 50°C, but is 50% active at 37°C. |
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Efficient cleavage requires at least two copies of the BspMI recognition sequence. Sticky ends from different BspMI sites may not be compatible. |
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Sticky ends from different BspQI sites may not be compatible. |
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Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot. |
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Sticky ends from different PasI sites may not be compatible. |
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PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations. |
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