pGAPZ(alpha) C
Pichia pastoris vector for constitutive expression of a secreted protein. For other reading frames, use pGAPZα A or pGAPZα B.
Sequence Author: Thermo Fisher (Invitrogen)
Explore Over 2.7k Plasmids: Yeast Plasmids | More Plasmid Sets
No matches
|
| ||
Sticky ends from different AlwNI sites may not be compatible. |
| ||
The 1-base overhangs produced by BciVI may be hard to ligate.Sticky ends from different BciVI sites may not be compatible. |
|
|
| ||
PciI is inhibited by nonionic detergents. |
|
| ||
BsrGI is typically used at 37°C, but is even more active at 60°C. |
|
| ||
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
| ||
| * Blocked by Dcm methylation. |
| ||
Sticky ends from different KflI sites may not be compatible. |
| ||
Sticky ends from different DraIII sites may not be compatible. |
| ||
FseI gradually loses activity when stored at -20°C. |
| ||
Sticky ends from different CsiI sites may not be compatible. |
| ||
| * Blocked by Dcm methylation. Sticky ends from different SexAI sites may not be compatible. |
| ||
Efficient cleavage requires at least two copies of the SgrAI recognition sequence. |
| ||
SmaI can be used at 37°C for brief incubations. |
|
| ||
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
|
|
| ||
BssHII is typically used at 50°C, but is 75% active at 37°C. |
|
|
|
|
|
| ||
Sticky ends from different TsoI sites may not be compatible.After cleavage, TsoI can remain bound to DNA and alter its electrophoretic mobility.For full activity, add fresh S-adenosylmethionine (SAM). |
| ||
Efficient cleavage requires at least two copies of the BfuAI recognition sequence. Sticky ends from different BfuAI sites may not be compatible.BfuAI is typically used at 50°C, but is 50% active at 37°C. |
| ||
Efficient cleavage requires at least two copies of the BspMI recognition sequence. Sticky ends from different BspMI sites may not be compatible. |
|