Pichia pastoris vector for regulated expression of secreted recombinant proteins with a cleavable FLAG®-6xHis-SUMO tag.
Explore Over 2.7k Plasmids: Yeast Plasmids | More Plasmid Sets
Sticky ends from different AlwNI sites may not be compatible.
After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
PciI is inhibited by nonionic detergents.
BsrGI is typically used at 37°C, but is even more active at 60°C.
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
|* Blocked by Dcm methylation.|
Sticky ends from different KflI sites may not be compatible.
Sticky ends from different DraIII sites may not be compatible.