pY-secSUMOstar

Pichia pastoris vector for regulated expression of secreted recombinant proteins with a cleavable FLAG®-6xHis-SUMO tag.

Sequence Author: LifeSensors

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AlwNI (3581) PspFI (3473) BseYI (3469) BssSαI (3338) PciI (3165) MluI (3076) BsrGI (3069) EcoRV (2902) StuI * (2868) KflI (2860) DraIII (2817) EagI (2795) FseI (2740) BanI (2634) SexAI * (2627) SgrAI (2578) SmaI (2554) TspMI - XmaI (2552) AatII (2510) ZraI (2508) BssHII - MauBI (2500) MscI (2467) NcoI - StyI (2462) BtgZI (2037) BamHI (1978) AleI - MslI (76) BmrI (178) PflMI (190) Eco53kI (206) BanII - SacI (208) PmeI (413) NsiI (677) BstXI - XcmI (706) MfeI (864) HindIII (872) BstBI (933) BstAPI (995) PsiI (1136) BsrDI (1155) PaeR7I - XhoI (1184) PstI (1210) 6xHis BsmBI (1548) SphI (1564) BsmBI - XbaI (1572) SalI (1615) AccI (1616) AgeI (1697) BsiWI (1829) PvuII (1863) pY-secSUMOstar 3894 bp
AlwNI  (3581)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PspFI  (3473)
1 site
C C C A G C G G G T C G
BseYI  (3469)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
BssSαI  (3338)
1 site
C A C G A G G T G C T C
PciI  (3165)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
MluI  (3076)
1 site
A C G C G T T G C G C A
BsrGI  (3069)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
EcoRV  (2902)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
StuI  (2868)
1 site
A G G C C T T C C G G A
* Blocked by Dcm methylation.
KflI  (2860)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
DraIII  (2817)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
EagI  (2795)
1 site
C G G C C G G C C G G C
FseI  (2740)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
BanI  (2634)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
SexAI  (2627)
1 site
A C C W G G