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pY-secSUMOstar
Pichia pastoris vector for regulated expression of secreted recombinant proteins with a cleavable FLAG®-6xHis-SUMO tag.
Sequence Author: LifeSensors
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Plasmids
Yeast Plasmids
pY-secSUMOstar
Show Static Map
AlwNI
(3581)
PspFI
(3473)
BseYI
(3469)
BssSαI
(3338)
PciI
(3165)
MluI
(3076)
BsrGI
(3069)
EcoRV
(2902)
StuI
*
(2868)
KflI
(2860)
DraIII
(2817)
EagI
(2795)
FseI
(2740)
BanI
(2634)
SexAI
*
(2627)
SgrAI
(2578)
SmaI
(2554)
TspMI
-
XmaI
(2552)
AatII
(2510)
ZraI
(2508)
BssHII
-
MauBI
(2500)
MscI
(2467)
NcoI
-
StyI
(2462)
BtgZI
(2037)
BamHI
(1978)
AleI
-
MslI
(76)
BmrI
(178)
PflMI
(190)
Eco53kI
(206)
BanII
-
SacI
(208)
PmeI
(413)
NsiI
(677)
BstXI
-
XcmI
(706)
MfeI
(864)
HindIII
(872)
BstBI
(933)
BstAPI
(995)
PsiI
(1136)
BsrDI
(1155)
PaeR7I
-
XhoI
(1184)
PstI
(1210)
6xHis
BsmBI
(1548)
SphI
(1564)
BsmBI
-
XbaI
(1572)
SalI
(1615)
AccI
(1616)
AgeI
(1697)
BsiWI
(1829)
PvuII
(1863)
AleI
-
(76)
AleI
-
(76)
AleI
-
MslI
(76)
MslI
(76)
MslI
BmrI
(178)
BmrI
(178)
BmrI
PflMI
(190)
PflMI
(190)
PflMI
Eco53kI
(206)
Eco53kI
(206)
Eco53kI
SacI
(208)
SacI
(208)
SacI
BanII
-
(208)
BanII
-
(208)
BanII
-
PmeI
(413)
PmeI
(413)
PmeI
NsiI
(677)
NsiI
(677)
NsiI
XcmI
(706)
XcmI
(706)
XcmI
BstXI
-
(706)
BstXI
-
(706)
BstXI
-
MfeI
(864)
MfeI
(864)
MfeI
HindIII
(872)
HindIII
(872)
HindIII
BstBI
(933)
BstBI
(933)
BstBI
BstAPI
(995)
BstAPI
(995)
BstAPI
PsiI
(1136)
PsiI
(1136)
PsiI
BsrDI
(1155)
BsrDI
(1155)
BsrDI
XhoI
(1184)
XhoI
(1184)
XhoI
PaeR7I
-
(1184)
PaeR7I
-
(1184)
PaeR7I
-
PstI
(1210)
PstI
(1210)
PstI
BsmBI
(1548)
(1572)
BsmBI
(1548)
BsmBI
-
(1572)
BsmBI
SphI
(1564)
SphI
(1564)
SphI
(1548)
BsmBI
-
(1572)
BsmBI
(1548)
BsmBI
-
(1572)
BsmBI
-
XbaI
(1572)
XbaI
(1572)
XbaI
SalI
(1615)
SalI
(1615)
SalI
AccI
(1616)
AccI
(1616)
AccI
AgeI
(1697)
AgeI
(1697)
AgeI
BsiWI
(1829)
BsiWI
(1829)
BsiWI
PvuII
(1863)
PvuII
(1863)
PvuII
BamHI
(1978)
BamHI
(1978)
BamHI
BtgZI
(2037)
BtgZI
(2037)
BtgZI
NcoI
-
(2462)
NcoI
-
(2462)
NcoI
-
StyI
(2462)
StyI
(2462)
StyI
MscI
(2467)
MscI
(2467)
MscI
BssHII
-
(2500)
BssHII
-
(2500)
BssHII
-
MauBI
(2500)
MauBI
(2500)
MauBI
ZraI
(2508)
ZraI
(2508)
ZraI
AatII
(2510)
AatII
(2510)
AatII
XmaI
(2552)
XmaI
(2552)
XmaI
TspMI
-
(2552)
TspMI
-
(2552)
TspMI
-
SmaI
(2554)
SmaI
(2554)
SmaI
SgrAI
(2578)
SgrAI
(2578)
SgrAI
SexAI
*
(2627)
SexAI
*
(2627)
SexAI
*
BanI
(2634)
BanI
(2634)
BanI
FseI
(2740)
FseI
(2740)
FseI
EagI
(2795)
EagI
(2795)
EagI
DraIII
(2817)
DraIII
(2817)
DraIII
KflI
(2860)
KflI
(2860)
KflI
StuI
*
(2868)
StuI
*
(2868)
StuI
*
EcoRV
(2902)
EcoRV
(2902)
EcoRV
BsrGI
(3069)
BsrGI
(3069)
BsrGI
MluI
(3076)
MluI
(3076)
MluI
PciI
(3165)
PciI
(3165)
PciI
BssSαI
(3338)
BssSαI
(3338)
BssSαI
BseYI
(3469)
BseYI
(3469)
BseYI
PspFI
(3473)
PspFI
(3473)
PspFI
AlwNI
(3581)
AlwNI
(3581)
AlwNI
6xHis
6xHis
6xHis
6xHis
pY-secSUMOstar
3894 bp
AlwNI (3581)
1 site
C
A
G
N
N
N
C
T
G
G
T
C
N
N
N
G
A
C
Sticky ends from different AlwNI sites may not be compatible.
PspFI (3473)
1 site
C
C
C
A
G
C
G
G
G
T
C
G
BseYI (3469)
1 site
C
C
C
A
G
C
G
G
G
T
C
G
After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
BssSαI (3338)
1 site
C
A
C
G
A
G
G
T
G
C
T
C
PciI (3165)
1 site
A
C
A
T
G
T
T
G
T
A
C
A
PciI is inhibited by nonionic detergents.
MluI (3076)
1 site
A
C
G
C
G
T
T
G
C
G
C
A
BsrGI (3069)
1 site
T
G
T
A
C
A
A
C
A
T
G
T
BsrGI is typically used at 37°C, but is even more active at 60°C.
EcoRV (2902)
1 site
G
A
T
A
T
C
C
T
A
T
A
G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
StuI (2868)
1 site
A
G
G
C
C
T
T
C
C
G
G
A
* Blocked by Dcm methylation.
KflI (2860)
1 site
G
G
G
W
C
C
C
C
C
C
W
G
G
G
Sticky ends from different KflI sites may not be compatible.
DraIII (2817)
1 site
C
A
C
N
N
N
G
T
G
G
T
G
N
N
N
C
A
C
Sticky ends from different DraIII sites may not be compatible.
EagI (2795)
1 site
C
G
G
C
C
G
G
C
C
G
