pPIC6(alpha) B
Pichia pastoris vector for methanol-inducible expression of a secreted protein. For other reading frames, use pPIC6α A or pPIC6α C.
Sequence Author: Thermo Fisher (Invitrogen)
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Sticky ends from different AlwNI sites may not be compatible. |
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PciI is inhibited by nonionic detergents. |
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BsrGI is typically used at 37°C, but is even more active at 60°C. |
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This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C. |
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EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
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| * Blocked by Dcm methylation. |
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Sticky ends from different KflI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the NmeAIII recognition sequence. Sticky ends from different NmeAIII sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM). |
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This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site. |
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Sticky ends from different DraIII sites may not be compatible. |
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Sticky ends from different TsoI sites may not be compatible.After cleavage, TsoI can remain bound to DNA and alter its electrophoretic mobility.For full activity, add fresh S-adenosylmethionine (SAM). |
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| * Blocked by Dam methylation. |
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Efficient cleavage requires at least two copies of the BsgI recognition sequence. Sticky ends from different BsgI sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM). |
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Sticky ends from different BtsαI sites may not be compatible. |
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Sticky ends from different StyI sites may not be compatible. |
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Sticky ends from different PflMI sites may not be compatible. |
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Sticky ends from different BstXI sites may not be compatible. |
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The 1-base overhangs produced by XcmI may be hard to ligate.Sticky ends from different XcmI sites may not be compatible. |
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Sticky ends from different BstAPI sites may not be compatible. |
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