pFA6a-GST-His3MX6

Plasmid with a HIS3MX6 marker for adding a C-terminal GST tag.
|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Yeast Plasmids | More Plasmid Sets
No matches
HindIII (19) NdeI (4576) PfoI (4438) AatII (4327) ZraI (4325) SspI (4209) PvuI (3775) NmeAIII (3553) BmrI (3445) BanI (3353) AlwNI (2928) PspFI (2820) BseYI (2816) HpaI (2333) BsiWI (25) SalI (37) F2 (42 .. 61) BamHI (43) AvaI - BsoBI - TspMI - XmaI (48) SmaI (50) PacI (58) EcoNI (73) MscI (270) BsgI (349) BstBI (460) SwaI (490) AscI (739) BstZ17I (817) BglII (947) BstEII (977) BmgBI (1030) Bpu10I (1039) BseRI (1141) MluI (1194) NcoI - StyI (1334) SphI (1462) BsaBI (1595) NgoMIV (1663) NaeI (1665) XbaI (1714) AfeI (1902) PmeI (2226) Eco53kI (2233) BanII - SacI (2235) EcoRI (2237) R1 (2223 .. 2242) EcoRV (2251) SfiI (2274) SacII (2281) pFA6a-GST-His3MX6 4659 bp
HindIII  (19)
1 site
A A G C T T T T C G A A
NdeI  (4576)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
PfoI  (4438)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
AatII  (4327)
1 site
G A C G T C C T G C A G
ZraI  (4325)
1 site
G A C G T C C T G C A G
SspI  (4209)
1 site
A A T A T T T T A T A A
PvuI  (3775)
1 site
C G A T C G G C T A G C
NmeAIII  (3553)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BmrI  (3445)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
BanI  (3353)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
AlwNI  (2928)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PspFI  (2820)
1 site
C C C A G C G G G T C G
BseYI  (2816)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
HpaI  (2333)
1 site
G T T A A C C A A T T G
BsiWI  (25)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
SalI  (37)
1 site
G T C G A C C A G C T G
BamHI  (43)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
AvaI  (48)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (48)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
TspMI  (48)
1 site
C C C G G G G G G C C C
XmaI  (48)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (50)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
PacI  (58)
1 site
T T A A T T A A A A T T A A T T
EcoNI  (73)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
MscI  (270)
1 site
T G G C C A A C C G G T
BsgI  (349)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BstBI  (460)
1 site
T T C G A A A A G C T T
SwaI  (490)
1 site
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
AscI  (739)
1 site
G G C G C G C C C C G C G C G G
BstZ17I  (817)
1 site
G T A T A C C A T A T G
BglII  (947)
1 site
A G A T C T T C T A G A
BstEII  (977)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BmgBI  (1030)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
Bpu10I  (1039)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BseRI  (1141)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
MluI  (1194)
1 site
A C G C G T T G C G C A
NcoI  (1334)
1 site
C C A T G G G G T A C C
StyI  (1334)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
SphI  (1462)
1 site
G C A T G C C G T A C G
BsaBI  (1595)
1 site
G A T N N N N A T C C T A N N N N T A G
NgoMIV  (1663)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
NaeI  (1665)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
XbaI  (1714)
1 site
T C T A G A A G A T C T
AfeI  (1902)
1 site
A G C G C T T C G C G A
PmeI  (2226)
1 site
G T T T A A A C C A A A T T T G
Eco53kI  (2233)
1 site
G A G C T C C T C G A G
BanII  (2235)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
SacI  (2235)
1 site
G A G C T C C T C G A G
EcoRI  (2237)
1 site
G A A T T C C T T A A G
EcoRV  (2251)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
SfiI  (2274)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
SacII  (2281)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
F2
20-mer  /  50% GC
1 binding site
42 .. 61  =  20 annealed bases
Tm  =  56°C
Forward primer for C-terminal tagging. This primer includes a BamHI recognition sequence. A gene-specific sequence should be added at the 5' end of the primer.
R1
20-mer  /  40% GC
1 binding site
2223 .. 2242  =  20 annealed bases
Tm  =  53°C
Reverse primer for gene deletion or C-terminal tagging. This primer includes an EcoRI recognition sequence. A gene-specific sequence should be added at the 5' end of the primer.
HIS3MX6
992 .. 2192  =  1201 bp
HIS3MX6
992 .. 2192  =  1201 bp
AmpR
3332 .. 4192  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   3332 .. 4123  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3332 .. 4192  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   4124 .. 4192  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3332 .. 4192  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
GST
63 .. 716  =  654 bp
218 amino acids  =  25.5 kDa
Product: glutathione S-transferase from Schistosoma japonicum
GST
63 .. 716  =  654 bp
218 amino acids  =  25.5 kDa
Product: glutathione S-transferase from Schistosoma japonicum
ori
2573 .. 3161  =  589 bp
high-copy-number colE1/pMB1/pBR322/pUC origin of replication
ori
2573 .. 3161  =  589 bp
high-copy-number colE1/pMB1/pBR322/pUC origin of replication
ADH1 terminator
758 .. 945  =  188 bp
ADH1 terminator
758 .. 945  =  188 bp
AmpR promoter
4193 .. 4297  =  105 bp
AmpR promoter
4193 .. 4297  =  105 bp
T7 promoter
2297 .. 2315  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
2297 .. 2315  =  19 bp
promoter for bacteriophage T7 RNA polymerase
SP6 promoter
4643 .. 2  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
SP6 promoter
4643 .. 2  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
S. pombe his5
1336 .. 1989  =  654 bp
217 amino acids  =  23.6 kDa
Product: imidazoleglycerol-phosphate dehydratase, required for histidine biosynthesis
yeast auxotrophic marker; corresponds to S. cerevisiae HIS3
S. pombe his5
1336 .. 1989  =  654 bp
217 amino acids  =  23.6 kDa
Product: imidazoleglycerol-phosphate dehydratase, required for histidine biosynthesis
yeast auxotrophic marker; corresponds to S. cerevisiae HIS3
TEF promoter
992 .. 1334  =  343 bp
Ashbya gossypii TEF promoter
TEF promoter
992 .. 1334  =  343 bp
Ashbya gossypii TEF promoter
TEF terminator
1995 .. 2192  =  198 bp
Ashbya gossypii TEF terminator
TEF terminator
1995 .. 2192  =  198 bp
Ashbya gossypii TEF terminator
ORF:  1336 .. 1989  =  654 bp
ORF:  217 amino acids  =  23.6 kDa
ORF:  63 .. 737  =  675 bp
ORF:  224 amino acids  =  26.2 kDa
ORF:  912 .. 1202  =  291 bp
ORF:  96 amino acids  =  10.9 kDa
ORF:  3462 .. 3728  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  3332 .. 4192  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
Click here to try SnapGene

Download pFA6a-GST-His3MX6.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.