Version 2.7.2

Changes in version 2.7.2 (Apr 22, 2015)


  • You can now use Cmd+D on Mac to activate “Don’t Save” when closing a file with unsaved changes.
    (Requested by Ronald Chalmers)
  • Added MW Markers from Axygen.
    (Requested by Martin)
  • Added MW Markers from Biozym.
    (Requested by Maximilian Heumüller)
  • Improved detection of lacZ-alpha.
    (Requested by Joëlle Fourment)
  • Improved default placement of windows.
    (Requested by Donelson Smith)


  • Fixed a crash that could occur while using the Simulate Agarose Gel dialog and switching between showing simplified and full primer duplex structures.
    (Reported by Claudia Garciadiaz)
  • Fixed a regression where the option of regenerating the enzymes used to digest the vector was not offered when designing primers for In-Fusion® cloning.
    (Reported by Alexander Wood)
  • Enabled unsaved annotations to be reflected in the history and transferred to the product after using a fragment directly when simulating Gibson Assembly® or In-Fusion® cloning.
    (Reported by Dan Kraut)
  • Improved the display of binding sites for certain primers with internal loops.
    (Reported by Seth Goldman)
  • Fixed an issue where designed primers sometimes did not use the desired and required Tm.
    (Reported by Rogerio Almeida)
  • Improved the simulation of Gibson Assembly® using fragments with 3′ A or G overhangs.
    (Reported by Nate Good)
  • Fixed an issue to ensure a correctly generated Entry Clone when linearizing the attB insert using the BP Cloning and BP + LR Cloning dialogs.
    (Reported by Rachael McCloy)
  • Fixed an issue that sometimes prevented simulating BP cloning when digesting the BP insert with an enzyme that cuts multiple times.
  • Removed a partially shown color button in the side toolbar of the Primer and Edit DNA Ends dialogs.
  • Enabled activation of the Destroy Restriction Site dialog by selecting a site at the numerical origin and pressing the Delete key.
  • Updated KflI to indicate that it is insensitive to Dcm methylation.
  • Fixed a crash that could occur after returning to interact with History view after resurrecting an ancestral sequence.
  • Implemented an improved crash reporter for Mac OS X Yosemite.
  • Fixed a potential crash in Features and Primers views when modifying annotations.
  • Configured the color mode and visibility controls in the Map & Sequence Options dialog to be hidden when using an embedded sequence, e.g., while using a cloning dialog.
  • Fixed an issue where using a mouse wheel to scroll up or down after showing the zoom controls could result in exiting zoomed mode.
  • Fixed an issue where it was not possible to click on feature names outside a circular map or above a linear map if shown enzymes and primers were both turned off.
  • Improved the output from Gibson Assembly® when creating a linear product for which the first amplified fragment wrapped around the numerical origin.
  • Ensured that after using Gibson Assembly® to assemble a linear product, sticky overhangs and endpoint modifications in the first and last fragment migrate to the product.
  • Enabled Gibson Assembly® of a linear product when either end of the product is covalently closed.

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