You can now use Cmd+D on Mac to activate “Don’t Save” when closing a file with unsaved changes.
(Requested by Ronald Chalmers)
Added MW Markers from Axygen.
(Requested by Martin)
Added MW Markers from Biozym.
(Requested by Maximilian Heumüller)
Improved detection of lacZ-alpha.
(Requested by Joëlle Fourment)
Improved default placement of windows.
(Requested by Donelson Smith)
Fixed a crash that could occur while using the Simulate Agarose Gel dialog and switching between showing simplified and full primer duplex structures.
(Reported by Claudia Garciadiaz)
Fixed a regression where the option of regenerating the enzymes used to digest the vector was not offered when designing primers for In-Fusion® cloning.
(Reported by Alexander Wood)
Enabled unsaved annotations to be reflected in the history and transferred to the product after using a fragment directly when simulating Gibson Assembly® or In-Fusion® cloning.
(Reported by Dan Kraut)
Improved the display of binding sites for certain primers with internal loops.
(Reported by Seth Goldman)
Fixed an issue where designed primers sometimes did not use the desired and required Tm.
(Reported by Rogerio Almeida)
Improved the simulation of Gibson Assembly® using fragments with 3′ A or G overhangs.
(Reported by Nate Good)
Fixed an issue to ensure a correctly generated Entry Clone when linearizing the attB insert using the BP Cloning and BP + LR Cloning dialogs.
(Reported by Rachael McCloy)
Fixed an issue that sometimes prevented simulating BP cloning when digesting the BP insert with an enzyme that cuts multiple times.
Removed a partially shown color button in the side toolbar of the Primer and Edit DNA Ends dialogs.
Enabled activation of the Destroy Restriction Site dialog by selecting a site at the numerical origin and pressing the Delete key.
Updated KflI to indicate that it is insensitive to Dcm methylation.
Fixed a crash that could occur after returning to interact with History view after resurrecting an ancestral sequence.
Implemented an improved crash reporter for Mac OS X Yosemite.
Fixed a potential crash in Features and Primers views when modifying annotations.
Configured the color mode and visibility controls in the Map & Sequence Options dialog to be hidden when using an embedded sequence, e.g., while using a cloning dialog.
Fixed an issue where using a mouse wheel to scroll up or down after showing the zoom controls could result in exiting zoomed mode.
Fixed an issue where it was not possible to click on feature names outside a circular map or above a linear map if shown enzymes and primers were both turned off.
Improved the output from Gibson Assembly® when creating a linear product for which the first amplified fragment wrapped around the numerical origin.
Ensured that after using Gibson Assembly® to assemble a linear product, sticky overhangs and endpoint modifications in the first and last fragment migrate to the product.
Enabled Gibson Assembly® of a linear product when either end of the product is covalently closed.