Pairs of DNA or protein sequences can be analyzed by local, global, or semi-global alignment.

Gene or transcript data from the Ensembl genome browser can now be imported directly into SnapGene.

A new interface simulates directional TOPO^® cloning into topoisomerase-activated vectors.

The interface for aligning to a reference DNA sequence has been enhanced. Controls for various display options are more intuitive, and alignment can be restricted to a designated strand or region of the reference sequence.

For a sequence that has been partially aligned to a reference DNA sequence, the nonaligned end portions can now be dragged out and visualized.

The Anza™ enzyme system from Thermo Fisher (Invitrogen) has been incorporated into SnapGene’s enzyme database.

The background color for the SnapGene interface can be changed.

Features can be shown or hidden based on feature type.

Amino acids in a translated feature can be set to lowercase, and a stretch of amino acids can be copied in 3-letter format.

A codon frequencies table can be generated for one or more translated features.

A selected region of a DNA alignment can be translated to generate the corresponding protein alignment.

When a cDNA is aligned to a reference genomic DNA sequence, the cDNA can be used to create a feature in which gaps are annotated as introns.

Selections are now marked with extended lines, which clarify the selection endpoints and also enhance visibility for small selections.