MS2-P65-HSF1_GFP

Zhang lab vector for expressing GFP together with an MS2 RNA-binding domain fused to the p65 and HSF1 transcription factors.

Sequence Author: Zhang Lab / Addgene #61423

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5' LTR (truncated) CMV enhancer MS2-P65-HSF1_GFP 11,409 bp
ATG
3872 .. 3874  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
3872 .. 3874  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
MS2-N55K
3875 .. 4261  =  387 bp
129 amino acids  =  13.7 kDa
Product: bacteriophage MS2 coat protein
binds with high affinity to a specific stem-loop structure in the viral RNA (Lim et al., 1994)
MS2-N55K
3875 .. 4261  =  387 bp
129 amino acids  =  13.7 kDa
Product: bacteriophage MS2 coat protein
binds with high affinity to a specific stem-loop structure in the viral RNA (Lim et al., 1994)
SV40 NLS
4316 .. 4336  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
4316 .. 4336  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
p65
4352 .. 4894  =  543 bp
181 amino acids  =  18.5 kDa
Product: C-terminal portion of the p65 subunit of mouse NF-κB
p65
4352 .. 4894  =  543 bp
181 amino acids  =  18.5 kDa
Product: C-terminal portion of the p65 subunit of mouse NF-κB
HSF1
4919 .. 5290  =  372 bp
124 amino acids  =  13.0 kDa
Product: C-terminal activation domain from the human heat shock transcription factor HSF1
HSF1
4919 .. 5290  =  372 bp
124 amino acids  =  13.0 kDa
Product: C-terminal activation domain from the human heat shock transcription factor HSF1
T2A
5306 .. 5359  =  54 bp
18 amino acids  =  1.8 kDa
Product: 2A peptide from Thosea asigna virus capsid protein
Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.
T2A
5306 .. 5359  =  54 bp
18 amino acids  =  1.8 kDa
Product: 2A peptide from Thosea asigna virus capsid protein
Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.
EGFP
5360 .. 6076  =  717 bp
238 amino acids  =  26.8 kDa
2 segments
   Segment 1:  1a  
   5360 .. 5362  =  3 bp
   1 amino acid  =  117.1 Da
Product: enhanced GFP
mammalian codon-optimized
EGFP
5360 .. 6076  =  717 bp
238 amino acids  =  26.8 kDa
2 segments
   Segment 2:  
   5363 .. 6076  =  714 bp
   237 amino acids  =  26.7 kDa
Product: enhanced GFP
mammalian codon-optimized
EGFP
5360 .. 6076  =  717 bp
238 amino acids  =  26.8 kDa
2 segments
Product: enhanced GFP
mammalian codon-optimized
EF-1α promoter
2682 .. 3859  =  1178 bp
strong constitutive promoter for human elongation factor EF-1α
EF-1α promoter
2682 .. 3859  =  1178 bp
strong constitutive promoter for human elongation factor EF-1α
AmpR
10,416 .. 11,276  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   10,416 .. 11,207  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
10,416 .. 11,276  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   11,208 .. 11,276  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
10,416 .. 11,276  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
9657 .. 10,245  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
9657 .. 10,245  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
WPRE
6101 .. 6689  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
WPRE
6101 .. 6689  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
f1 ori
7696 .. 8124  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
7696 .. 8124  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
238 .. 617  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
238 .. 617  =  380 bp
human cytomegalovirus immediate early enhancer
BleoR
8581 .. 8955  =  375 bp
124 amino acids  =  13.8 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and Zeocin™
BleoR
8581 .. 8955  =  375 bp
124 amino acids  =  13.8 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and Zeocin™
SV40 promoter
8138 .. 8467  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
8138 .. 8467  =  330 bp
SV40 enhancer and early promoter
RRE
1680 .. 1913  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
RRE
1680 .. 1913  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
bGH poly(A) signal
7426 .. 7650  =  225 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
7426 .. 7650  =  225 bp
bovine growth hormone polyadenylation signal
CMV promoter
619 .. 817  =  199 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
619 .. 817  =  199 bp
human cytomegalovirus (CMV) immediate early promoter
5' LTR (truncated)
835 .. 1015  =  181 bp
truncated 5' long terminal repeat (LTR) from HIV-1
5' LTR (truncated)
835 .. 1015  =  181 bp
truncated 5' long terminal repeat (LTR) from HIV-1
5' LTR (truncated)
7214 .. 7394  =  181 bp
truncated 5' long terminal repeat (LTR) from HIV-1
5' LTR (truncated)
7214 .. 7394  =  181 bp
truncated 5' long terminal repeat (LTR) from HIV-1
HIV-1 Ψ
1062 .. 1187  =  126 bp
packaging signal of human immunodeficiency virus type 1
HIV-1 Ψ
1062 .. 1187  =  126 bp
packaging signal of human immunodeficiency virus type 1
SV40 poly(A) signal
9085 .. 9206  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
9085 .. 9206  =  122 bp
SV40 polyadenylation signal
cPPT/CTS
2440 .. 2557  =  118 bp
central polypurine tract and central termination sequence of HIV-1
cPPT/CTS
2440 .. 2557  =  118 bp
central polypurine tract and central termination sequence of HIV-1
AmpR promoter
11,277 .. 11,381  =  105 bp
AmpR promoter
11,277 .. 11,381  =  105 bp
EM7 promoter
8515 .. 8562  =  48 bp
synthetic bacterial promoter
EM7 promoter
8515 .. 8562  =  48 bp
synthetic bacterial promoter
EF-1α intron A
2913 .. 3850  =  938 bp
intron upstream of the start codon of human EF-1α
EF-1α intron A
2913 .. 3850  =  938 bp
intron upstream of the start codon of human EF-1α
SV40 ori
8318 .. 8453  =  136 bp
SV40 origin of replication
SV40 ori
8318 .. 8453  =  136 bp
SV40 origin of replication
ORF:  1558 .. 2445  =  888 bp
ORF:  295 amino acids  =  33.8 kDa
ORF:  3316 .. 3549  =  234 bp
ORF:  77 amino acids  =  7.7 kDa
ORF:  8581 .. 8955  =  375 bp
ORF:  124 amino acids  =  13.8 kDa
ORF:  10,546 .. 10,812  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  2420 .. 2803  =  384 bp
ORF:  127 amino acids  =  14.5 kDa
ORF:  3872 .. 6076  =  2205 bp
ORF:  734 amino acids  =  77.3 kDa
ORF:  6191 .. 7240  =  1050 bp
ORF:  349 amino acids  =  39.4 kDa
ORF:  3237 .. 3674  =  438 bp
ORF:  145 amino acids  =  15.9 kDa
ORF:  6204 .. 6716  =  513 bp
ORF:  170 amino acids  =  18.0 kDa
ORF:  7647 .. 7913  =  267 bp
ORF:  88 amino acids  =  9.3 kDa
ORF:  4699 .. 4983  =  285 bp
ORF:  94 amino acids  =  9.6 kDa
ORF:  1473 .. 1853  =  381 bp
ORF:  126 amino acids  =  13.3 kDa
ORF:  2598 .. 3599  =  1002 bp
ORF:  333 amino acids  =  36.1 kDa
ORF:  8574 .. 8861  =  288 bp
ORF:  95 amino acids  =  10.1 kDa
ORF:  10,416 .. 11,276  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
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