MS2-P65-HSF1_GFP
Zhang lab vector for expressing GFP together with an MS2 RNA-binding domain fused to the p65 and HSF1 transcription factors.
Sequence Author: Zhang Lab / Addgene #61423
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| ATG 3872 .. 3874 = 3 bp 1 amino acid = 149.2 Da Product: start codon |
| ATG 3872 .. 3874 = 3 bp 1 amino acid = 149.2 Da Product: start codon |
| MS2-N55K 3875 .. 4261 = 387 bp 129 amino acids = 13.7 kDa Product: bacteriophage MS2 coat protein binds with high affinity to a specific stem-loop structure in the viral RNA (Lim et al., 1994) |
| MS2-N55K 3875 .. 4261 = 387 bp 129 amino acids = 13.7 kDa Product: bacteriophage MS2 coat protein binds with high affinity to a specific stem-loop structure in the viral RNA (Lim et al., 1994) |
| SV40 NLS 4316 .. 4336 = 21 bp 7 amino acids = 883.1 Da Product: nuclear localization signal of SV40 large T antigen |
| SV40 NLS 4316 .. 4336 = 21 bp 7 amino acids = 883.1 Da Product: nuclear localization signal of SV40 large T antigen |
| p65 4352 .. 4894 = 543 bp 181 amino acids = 18.5 kDa Product: C-terminal portion of the p65 subunit of mouse NF-κB |
| p65 4352 .. 4894 = 543 bp 181 amino acids = 18.5 kDa Product: C-terminal portion of the p65 subunit of mouse NF-κB |
| HSF1 4919 .. 5290 = 372 bp 124 amino acids = 13.0 kDa Product: C-terminal activation domain from the human heat shock transcription factor HSF1 |
| HSF1 4919 .. 5290 = 372 bp 124 amino acids = 13.0 kDa Product: C-terminal activation domain from the human heat shock transcription factor HSF1 |
| T2A 5306 .. 5359 = 54 bp 18 amino acids = 1.8 kDa Product: 2A peptide from Thosea asigna virus capsid protein Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides. |
| T2A 5306 .. 5359 = 54 bp 18 amino acids = 1.8 kDa Product: 2A peptide from Thosea asigna virus capsid protein Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides. |
| EGFP 5360 .. 6076 = 717 bp 238 amino acids = 26.8 kDa 2 segments Segment 1: 1a 5360 .. 5362 = 3 bp 1 amino acid = 117.1 Da Product: enhanced GFP mammalian codon-optimized |
| EGFP 5360 .. 6076 = 717 bp 238 amino acids = 26.8 kDa 2 segments Segment 2: 5363 .. 6076 = 714 bp 237 amino acids = 26.7 kDa Product: enhanced GFP mammalian codon-optimized |
| EGFP 5360 .. 6076 = 717 bp 238 amino acids = 26.8 kDa 2 segments Product: enhanced GFP mammalian codon-optimized |
| EF-1α promoter 2682 .. 3859 = 1178 bp strong constitutive promoter for human elongation factor EF-1α |
| EF-1α promoter 2682 .. 3859 = 1178 bp strong constitutive promoter for human elongation factor EF-1α |
| AmpR 10,416 .. 11,276 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 2: 10,416 .. 11,207 = 792 bp 263 amino acids = 28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| AmpR 10,416 .. 11,276 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 1: signal sequence 11,208 .. 11,276 = 69 bp 23 amino acids = 2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| AmpR 10,416 .. 11,276 = 861 bp 286 amino acids = 31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| ori 9657 .. 10,245 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| ori 9657 .. 10,245 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| WPRE 6101 .. 6689 = 589 bp woodchuck hepatitis virus posttranscriptional regulatory element |
| WPRE 6101 .. 6689 = 589 bp woodchuck hepatitis virus posttranscriptional regulatory element |
| f1 ori 7696 .. 8124 = 429 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
| f1 ori 7696 .. 8124 = 429 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
| CMV enhancer 238 .. 617 = 380 bp human cytomegalovirus immediate early enhancer |
| CMV enhancer 238 .. 617 = 380 bp human cytomegalovirus immediate early enhancer |
| BleoR 8581 .. 8955 = 375 bp 124 amino acids = 13.8 kDa Product: antibiotic-binding protein confers resistance to bleomycin, phleomycin, and Zeocin™ |
| BleoR 8581 .. 8955 = 375 bp 124 amino acids = 13.8 kDa Product: antibiotic-binding protein confers resistance to bleomycin, phleomycin, and Zeocin™ |
| SV40 promoter 8138 .. 8467 = 330 bp SV40 enhancer and early promoter |
| SV40 promoter 8138 .. 8467 = 330 bp SV40 enhancer and early promoter |
| RRE 1680 .. 1913 = 234 bp The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm. |
| RRE 1680 .. 1913 = 234 bp The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm. |
| bGH poly(A) signal 7426 .. 7650 = 225 bp bovine growth hormone polyadenylation signal |
| bGH poly(A) signal 7426 .. 7650 = 225 bp bovine growth hormone polyadenylation signal |
| CMV promoter 619 .. 817 = 199 bp human cytomegalovirus (CMV) immediate early promoter |
| CMV promoter 619 .. 817 = 199 bp human cytomegalovirus (CMV) immediate early promoter |
| 5' LTR (truncated) 835 .. 1015 = 181 bp truncated 5' long terminal repeat (LTR) from HIV-1 |
| 5' LTR (truncated) 835 .. 1015 = 181 bp truncated 5' long terminal repeat (LTR) from HIV-1 |
| 5' LTR (truncated) 7214 .. 7394 = 181 bp truncated 5' long terminal repeat (LTR) from HIV-1 |
| 5' LTR (truncated) 7214 .. 7394 = 181 bp truncated 5' long terminal repeat (LTR) from HIV-1 |
| HIV-1 Ψ 1062 .. 1187 = 126 bp packaging signal of human immunodeficiency virus type 1 |
| HIV-1 Ψ 1062 .. 1187 = 126 bp packaging si |