pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2

Lentiviral vector for labeling chromosomal loci by tetracycline-regulatable expression of catalytically dead Streptococcus thermophilus dCas9 fused to three copies of mTagBFP2.

Sequence Author: Pederson Lab / Addgene #64512

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: CRISPR Plasmids | More Plasmid Sets
No matches
SV40 NLS SV40 NLS CMV promoter tet operator tet operator ATG SV40 NLS pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2 12,284 bp
ATG
2980 .. 2982  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
2980 .. 2982  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
SV40 NLS
2986 .. 3006  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
2986 .. 3006  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
St1dCas9
3016 .. 6375  =  3360 bp
1120 amino acids  =  129.1 kDa
7 segments
   Segment 1:  
   3016 .. 3036  =  21 bp
   7 amino acids  =  715.8 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus thermophilus Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the mutations D9A, D598A, H599A, and N622A
St1dCas9
3016 .. 6375  =  3360 bp
1120 amino acids  =  129.1 kDa
7 segments
   Segment 2:  
   3037 .. 3039  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus thermophilus Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the mutations D9A, D598A, H599A, and N622A
St1dCas9
3016 .. 6375  =  3360 bp
1120 amino acids  =  129.1 kDa
7 segments
   Segment 3:  
   3040 .. 4803  =  1764 bp
   588 amino acids  =  67.5 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus thermophilus Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the mutations D9A, D598A, H599A, and N622A
St1dCas9
3016 .. 6375  =  3360 bp
1120 amino acids  =  129.1 kDa
7 segments
   Segment 4:  
   4804 .. 4809  =  6 bp
   2 amino acids  =  160.2 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus thermophilus Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the mutations D9A, D598A, H599A, and N622A
St1dCas9
3016 .. 6375  =  3360 bp
1120 amino acids  =  129.1 kDa
7 segments
   Segment 5:  
   4810 .. 4875  =  66 bp
   22 amino acids  =  2.4 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus thermophilus Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the mutations D9A, D598A, H599A, and N622A
St1dCas9
3016 .. 6375  =  3360 bp
1120 amino acids  =  129.1 kDa
7 segments
   Segment 6:  
   4876 .. 4878  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus thermophilus Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the mutations D9A, D598A, H599A, and N622A
St1dCas9
3016 .. 6375  =  3360 bp
1120 amino acids  =  129.1 kDa
7 segments
   Segment 7:  
   4879 .. 6375  =  1497 bp
   499 amino acids  =  58.3 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus thermophilus Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the mutations D9A, D598A, H599A, and N622A
St1dCas9
3016 .. 6375  =  3360 bp
1120 amino acids  =  129.1 kDa
7 segments
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus thermophilus Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the mutations D9A, D598A, H599A, and N622A
SV40 NLS
6391 .. 6411  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
6391 .. 6411  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
6424 .. 6444  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
6424 .. 6444  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
6451 .. 6471  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
6451 .. 6471  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
mTagBFP2
6484 .. 7194  =  711 bp
237 amino acids  =  26.7 kDa
Product: enhanced monomeric blue fluorescent protein (Subach et al., 2011)
mammalian codon-optimized
mTagBFP2
6484 .. 7194  =  711 bp
237 amino acids  =  26.7 kDa
Product: enhanced monomeric blue fluorescent protein (Subach et al., 2011)
mammalian codon-optimized
mTagBFP2
7213 .. 7923  =  711 bp
237 amino acids  =  26.7 kDa
Product: enhanced monomeric blue fluorescent protein (Subach et al., 2011)
mammalian codon-optimized
mTagBFP2
7213 .. 7923  =  711 bp
237 amino acids  =  26.7 kDa
Product: enhanced monomeric blue fluorescent protein (Subach et al., 2011)
mammalian codon-optimized
mTagBFP2
7942 .. 8652  =  711 bp
237 amino acids  =  26.7 kDa
Product: enhanced monomeric blue fluorescent protein (Subach et al., 2011)
mammalian codon-optimized
mTagBFP2
7942 .. 8652  =  711 bp
237 amino acids  =  26.7 kDa
Product: enhanced monomeric blue fluorescent protein (Subach et al., 2011)
mammalian codon-optimized
AmpR
9880 .. 10,740  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 1:  signal sequence  
   9880 .. 9948  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
9880 .. 10,740  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 2:  
   9949 .. 10,740  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
9880 .. 10,740  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
3' LTR
1 .. 634  =  634 bp
3' long terminal repeat (LTR) from HIV-1
3' LTR
1 .. 634  =  634 bp
3' long terminal repeat (LTR) from HIV-1
WPRE
8674 .. 9262  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
WPRE
8674 .. 9262  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
ori
10,911 .. 11,499  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
10,911 .. 11,499  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CMV enhancer
2299 .. 2602  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
2299 .. 2602  =  304 bp
human cytomegalovirus immediate early enhancer
RRE
1303 .. 1536  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
RRE
1303 .. 1536  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
3' LTR (ΔU3)
9337 .. 9570  =  234 bp
self-inactivating 3' long terminal repeat (LTR) from HIV-1
3' LTR (ΔU3)
9337 .. 9570  =  234 bp
self-inactivating 3' long terminal repeat (LTR) from HIV-1
CMV promoter
2603 .. 2806  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
2603 .. 2806  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
HIV-1 Ψ
681 .. 806  =  126 bp
packaging signal of human immunodeficiency virus type 1
HIV-1 Ψ
681 .. 806  =  126 bp
packaging signal of human immunodeficiency virus type 1
cPPT/CTS
2058 .. 2175  =  118 bp
central polypurine tract and central termination sequence of HIV-1
cPPT/CTS
2058 .. 2175  =  118 bp
central polypurine tract and central termination sequence of HIV-1
AmpR promoter
9775 .. 9879  =  105 bp
AmpR promoter
9775 .. 9879  =  105 bp
tet operator
2808 .. 2826  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
2808 .. 2826  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
2829 .. 2847  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
2829 .. 2847  =  19 bp
bacterial operator O2 for the tetR and tetA genes
ORF:  2980 .. 8661  =  5682 bp
ORF:  1893 amino acids  =  215.4 kDa
ORF:  8764 .. 9276  =  513 bp
ORF:  170 amino acids  =  18.6 kDa
ORF:  9880 .. 10,740  =  861 bp
ORF:  286 amino acids  =  31.5 kDa
ORF:  1181 .. 2107  =  927 bp
ORF:  308 amino acids  =  35.4 kDa
ORF:  2762 .. 3073  =  312 bp
ORF:  103 amino acids  =  12.3 kDa
ORF:  8777 .. 9310  =  534 bp
ORF:  177 amino acids  =  18.8 kDa
ORF:  1881 .. 2129  =  249 bp
ORF:  82 amino acids  =  9.0 kDa
ORF:  4140 .. 4415  =  276 bp
ORF:  91 amino acids  =  10.2 kDa
ORF:  4443 .. 4826  =  384 bp
ORF:  127 amino acids  =  14.6 kDa
ORF:  6300 .. 6539  =  240 bp
ORF:  79 amino acids  =  9.0 kDa
ORF:  10,344 .. 10,610  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  1015 .. 1476  =  462 bp
ORF:  153 amino acids  =  16.6 kDa
ORF:  6448 .. 8460  =  2013 bp
ORF:  670 amino acids  =  69.7 kDa
Click here to try SnapGene

Download pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps