pFGC-pcoCas9
Binary plant vector for expressing plant codon-optimized Case9 from the 35SPPDK promoter.
Sequence Author: Sheen Lab / Addgene #52256
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PaeR7I does not recognize the sequence CTCTCGAG. |
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SwaI is typically used at 25°C, but is 50% active at 37°C. |
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I-PpoI is a homing endonuclease that can recognize a variety of similar recognition sequences. |
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I-SceI is a homing endonuclease that can recognize a variety of similar recognition sequences.After cleavage, I-SceI can remain bound to DNA and alter its electrophoretic mobility. |
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I-CeuI is a homing endonuclease that can recognize a variety of similar recognition sequences. |
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Sticky ends from different Bsu36I sites may not be compatible. |
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PI-PspI is a homing endonuclease that can recognize a variety of similar recognition sequences. |
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SmaI can be used at 37°C for brief incubations. |
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Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
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ATG 5005 .. 5007 = 3 bp 1 amino acid = 149.2 Da Product: start codon |
ATG 5005 .. 5007 = 3 bp 1 amino acid = 149.2 Da Product: start codon |
2xFLAG 5008 .. 5055 = 48 bp 16 amino acids = 2.0 kDa Product: two tandem FLAG® epitope tags |
2xFLAG 5008 .. 5055 = 48 bp 16 amino acids = 2.0 kDa Product: two tandem FLAG® epitope tags |
SV40 NLS 5062 .. 5082 = 21 bp 7 amino acids = 883.1 Da Product: nuclear localization signal of SV40 large T antigen |
SV40 NLS 5062 .. 5082 = 21 bp 7 amino acids = 883.1 Da Product: nuclear localization signal of SV40 large T antigen |
pcoCas9 5107 .. 9396 = 4290 bp 1367 amino acids = 158.3 kDa 2 segments = 4101 bp Product: Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system plant codon-optimized Cas9 gene containing the potato IV2 intron |
pcoCas9 5107 .. 9396 = 4290 bp 1367 amino acids = 158.3 kDa 2 segments = 4101 bp Segment 1: 5107 .. 6543 = 1437 bp 479 amino acids = 55.4 kDa Product: Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system plant codon-optimized Cas9 gene contain |