pAAV-Guide-it-Down Vector (Linear)

CRISPR/Cas9 adeno-associated virus (AAV) vector for cloning and expression of a single guide RNA (sgRNA). Use together with pAAV-Guide-it-Up Vector.

Sequence Author: Clontech (TaKaRa)

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: CRISPR Plasmids | More Plasmid Sets
No matches
Guide-itâ„¢ Sequencing Primer 1 (6227 .. 6250) SV40 NLS polIII terminator pAAV-Guide-it-Down Vector 6306 bp
Guide-itâ„¢ Sequencing Primer 1
24-mer  /  38% GC
1 binding site
6227 .. 6250  =  24 annealed bases
Tm  =  56°C
Cas9-Down
2774 .. 5389  =  2616 bp
872 amino acids  =  101.2 kDa
Product: downstream fragment of Streptococcus pyogenes Cas9
Cas9-Down
2774 .. 5389  =  2616 bp
872 amino acids  =  101.2 kDa
Product: downstream fragment of Streptococcus pyogenes Cas9
SV40 NLS
5405 .. 5425  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
5405 .. 5425  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
FLAG
5426 .. 5449  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
FLAG
5426 .. 5449  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
AmpR
660 .. 1520  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   660 .. 728  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
660 .. 1520  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   729 .. 1520  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
660 .. 1520  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
1691 .. 2279  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1691 .. 2279  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
hGH poly(A) signal
5461 .. 5937  =  477 bp
human growth hormone polyadenylation signal
hGH poly(A) signal
5461 .. 5937  =  477 bp
human growth hormone polyadenylation signal
U6 promoter
6060 .. 6308  =  249 bp
RNA polymerase III promoter for human U6 snRNA (Domitrovich & Kunkel, 2003)
U6 promoter
6060 .. 6308  =  249 bp
RNA polymerase III promoter for human U6 snRNA (Domitrovich & Kunkel, 2003)
AAV2 ITR
153 .. 293  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AAV2 ITR
153 .. 293  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AAV2 ITR
2624 .. 2764  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AAV2 ITR
2624 .. 2764  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AmpR promoter
555 .. 659  =  105 bp
AmpR promoter
555 .. 659  =  105 bp
gRNA scaffold
1 .. 86  =  86 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
gRNA scaffold
1 .. 86  =  86 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
polIII terminator
87 .. 92  =  6 bp
RNA polymerase III transcription terminator
polIII terminator
87 .. 92  =  6 bp
RNA polymerase III transcription terminator
ORF:  167 .. 448  =  282 bp
ORF:  93 amino acids  =  9.9 kDa
ORF:  2885 .. 5452  =  2568 bp
ORF:  855 amino acids  =  99.0 kDa
ORF:  660 .. 1520  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  1124 .. 1390  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  3250 .. 3648  =  399 bp
ORF:  132 amino acids  =  15.6 kDa
Click here to try SnapGene

Download pAAV-Guide-it-Down Vector (Linear).dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.