pLV hUbC-Cas9-T2A-GFP
Lentiviral vector for co-expression of human codon-optimized Cas9 and enhanced GFP (EGFP).
Sequence Author: Gersbach Lab / Addgene #53190
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ATG 4369 .. 4371 = 3 bp 1 amino acid = 149.2 Da |
ATG 4369 .. 4371 = 3 bp 1 amino acid = 149.2 Da |
3xFLAG 4372 .. 4437 = 66 bp 22 amino acids = 2.7 kDa Product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site |
3xFLAG 4372 .. 4437 = 66 bp 22 amino acids = 2.7 kDa Product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site |
SV40 NLS 4444 .. 4464 = 21 bp 7 amino acids = 883.1 Da Product: nuclear localization signal of SV40 large T antigen |
SV40 NLS 4444 .. 4464 = 21 bp 7 amino acids = 883.1 Da Product: nuclear localization signal of SV40 large T antigen |
Cas9 4474 .. 8577 = 4104 bp 1368 amino acids = 158.4 kDa Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system generates RNA-guided double strand breaks in DNA |
Cas9 4474 .. 8577 = 4104 bp 1368 amino acids = 158.4 kDa Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system generates RNA-guided double strand breaks in DNA |
SV40 NLS 8590 .. 8610 = 21 bp 7 amino acids = 883.1 Da Product: nuclear localization signal of SV40 large T antigen |
SV40 NLS 8590 .. 8610 = 21 bp 7 amino acids = 883.1 Da Product: nuclear localization signal of SV40 large T antigen |
T2A 8617 .. 8670 = 54 bp 18 amino acids = 1.8 kDa Product: 2A peptide from Thosea asigna virus capsid protein Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides. |
T2A 8617 .. 8670 = 54 bp 18 amino acids = 1.8 kDa Product: 2A peptide from Thosea asigna virus capsid protein Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides. |
EGFP 8677 .. 9390 = 714 bp 238 amino acids = 26.8 kDa 2 segments Segment 1: 1a 8677 .. 8679 = 3 bp 1 amino acid = 117.1 Da Product: enhanced GFP mammalian codon-optimized |
EGFP 8677 .. 9390 = 714 bp 238 amino acids = 26.8 kDa 2 segments Segment 2: 8680 .. 9390 = 711 bp 237 amino acids = 26.7 kDa Product: enhanced GFP mammalian codon-optimized |
EGFP 8677 .. 9390 = 714 bp 238 amino acids = 26.8 kDa 2 segments Product: enhanced GFP mammalian codon-optimized |
UbC promoter 3124 .. 4334 = 1211 bp human ubiquitin C promoter |
UbC promoter 3124 .. 4334 = 1211 bp human ubiquitin C promoter |
AmpR 13,779 .. 14,639 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 2: 13,779 .. 14,570 = 792 bp 263 amino acids = 28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
AmpR 13,779 .. 14,639 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 1: signal sequence 14,571 .. 14,639 = 69 bp 23 amino acids = 2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
AmpR 13,779 .. 14,639 = 861 bp 286 amino acids = 31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
ori 13,020 .. 13,608 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
ori 13,020 .. 13,608 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
WPRE 9464 .. 10,052 = 589 bp woodchuck hepatitis virus posttranscriptional regulatory element |
WPRE 9464 .. 10,052 = 589 bp woodchuck hepatitis virus posttranscriptional regulatory element |
f1 ori 11,059 .. 11,487 = 429 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
f1 ori 11,059 .. 11,487 = 429 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
CMV enhancer 238 .. 617 = 380 bp human cytomegalovirus immediate early enhancer |
CMV enhancer 238 .. 617 = 380 bp human cytomegalovirus immediate early enhancer |
BleoR 11,944 .. 12,318 = 375 bp 124 amino acids = 13.8 kDa Product: antibiotic-binding protein confers resistance to bleomycin, phleomycin, and Zeocin™ |
BleoR 11,944 .. 12,318 = 375 bp 124 amino acids = 13.8 kDa Product: antibiotic-binding protein confers resistance to bleomycin, phleomycin, and Zeocin™ |
SV40 promoter 11,501 .. 11,830 = 330 bp SV40 enhancer and early promoter |
SV40 promoter 11,501 .. 11,830 = 330 bp SV40 enhancer and early promoter |
RRE 1680 .. 1913 = 234 bp The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm. |
RRE 1680 .. 1913 = 234 bp The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm. |
bGH poly(A) signal 10,789 .. 11,013 = 225 bp bovine growth hormone polyadenylation signal |
bGH poly(A) signal 10,789 .. 11,013 = 225 bp bovine growth hormone polyadenylation signal |
CMV promoter 619 .. 817 = 199 bp human cytomegalovirus (CMV) immediate early promoter |
CMV promoter 619 .. 817 = 199 bp human cytomegalovirus (CMV) immediate early promoter |
LTR (truncated) 10,577 .. 10,757 = 181 bp truncated long terminal repeat (LTR) from HIV-1 |
LTR (truncated) 10,577 .. 10,757 = 181 bp truncated long terminal repeat (LTR) from HIV-1 |
LTR (truncated) 835 .. 1015 = 181 bp truncated long terminal repeat (LTR) from HIV-1 |
LTR (truncated) 835 .. 1015 = 181 bp truncated long terminal repeat (LTR) from HIV-1 |
HIV-1 Ψ 1062 .. 1187 = 126 bp packaging signal of human immunodeficiency virus type 1 |
HIV-1 Ψ 1062 .. 1187 = 126 bp packaging signal of human immunodeficiency virus type 1 |
SV40 poly(A) signal 12,448 .. 12,569 = 122 bp SV40 polyadenylation signal |
SV40 poly(A) signal 12,448 .. 12,569 = 122 bp SV40 polyadenylation signal |
cPPT/CTS 2440 .. 2557 = 118 bp central polypurine tract and central termination sequence of HIV-1 |
cPPT/CTS 2440 .. 2557 = 118 bp central polypurine tract and central termination sequence of HIV-1 |
AmpR promoter 14,640 .. 14,744 = 105 bp |
AmpR promoter 14,640 .. 14,744 = 105 bp |
EM7 promoter 11,878 .. 11,925 = 48 bp synthetic bacterial promoter |
EM7 promoter 11,878 .. 11,925 = 48 bp synthetic bacterial promoter |
loxP 2602 .. 2635 = 34 bp Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT). |
loxP 2602 .. 2635 = 34 bp Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT). |
loxP 9406 .. 9439 = 34 bp Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT). |