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lentiCRISPR v2
Zhang lab lentiviral transfer plasmid, also known as pLentiCRISPR v2, for CRISPR/Cas9 genome editing of mammalian cells with a single guide RNA (sgRNA).
Sequence Author: Zhang Lab / Addgene #52961
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lentiCRISPR v2
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EM7 promoter
FLAG
CMV promoter
hU6-F
(2608 .. 2628)
LKO.1 5'
(2779 .. 2798)
BsmBI
(2852)
BsmBI
(4737)
polIII terminator
BsmBI
(2852)
(4737)
BsmBI
(2852)
BsmBI
(4737)
BsmBI
(2852)
BsmBI
(4737)
BsmBI
(2852)
BsmBI
(4737)
BsmBI
hU6-F
(2608 .. 2628)
hU6-F
hU6-F
(2608 .. 2628)
LKO.1 5'
(2779 .. 2798)
LKO.1 5'
LKO.1 5'
(2779 .. 2798)
FLAG
FLAG
FLAG
FLAG
CMV promoter
CMV promoter
CMV promoter
CMV promoter
EM7 promoter
EM7 promoter
EM7 promoter
EM7 promoter
polIII terminator
polIII terminator
polIII terminator
polIII terminator