lentiCas9n(D10A)-Blast
Zhang lab lentiviral vector with a blasticidin resistance marker, for expressing Cas9 with the D10A nickase mutation.
Sequence Author: Zhang Lab / Addgene #63593
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| Cas9(D10A) 2887 .. 6990 = 4104 bp 1368 amino acids = 158.4 kDa 3 segments Segment 1: 2887 .. 2913 = 27 bp 9 amino acids = 1.1 kDa Product: nickase mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system generates RNA-guided single strand nicks in DNA due to the D10A mutation in the RuvC catalytic domain |
| Cas9(D10A) 2887 .. 6990 = 4104 bp 1368 amino acids = 158.4 kDa 3 segments Segment 2: 2914 .. 2916 = 3 bp 1 amino acid = 89.1 Da Product: nickase mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system generates RNA-guided single strand nicks in DNA due to the D10A mutation in the RuvC catalytic domain |
| Cas9(D10A) 2887 .. 6990 = 4104 bp 1368 amino acids = 158.4 kDa 3 segments Segment 3: 2917 .. 6990 = 4074 bp 1358 amino acids = 157.3 kDa Product: nickase mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system generates RNA-guided single strand nicks in DNA due to the D10A mutation in the RuvC catalytic domain |
| Cas9(D10A) 2887 .. 6990 = 4104 bp 1368 amino acids = 158.4 kDa 3 segments Product: nickase mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system generates RNA-guided single strand nicks in DNA due to the D10A mutation in the RuvC catalytic domain |
| nucleoplasmin NLS 6991 .. 7038 = 48 bp 16 amino acids = 1.7 kDa Product: bipartite nuclear localization signal from nucleoplasmin |
| nucleoplasmin NLS 6991 .. 7038 = 48 bp 16 amino acids = 1.7 kDa Product: bipartite nuclear localization signal from nucleoplasmin |
| FLAG 7039 .. 7062 = 24 bp 8 amino acids = 1.0 kDa Product: FLAG® epitope tag, followed by an enterokinase cleavage site |
| FLAG 7039 .. 7062 = 24 bp 8 amino acids = 1.0 kDa Product: FLAG® epitope tag, followed by an enterokinase cleavage site |
| P2A 7072 .. 7128 = 57 bp 19 amino acids = 2.0 kDa Product: 2A peptide from porcine teschovirus-1 polyprotein Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides. |
| P2A 7072 .. 7128 = 57 bp 19 amino acids = 2.0 kDa Product: 2A peptide from porcine teschovirus-1 polyprotein Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides. |
| BSD 7129 .. 7527 = 399 bp 132 amino acids = 13.7 kDa Product: blasticidin S deaminase confers resistance to blasticidin |
| BSD 7129 .. 7527 = 399 bp 132 amino acids = 13.7 kDa Product: blasticidin S deaminase confers resistance to blasticidin |
| AmpR 11,867 .. 12,727 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 2: 11,867 .. 12,658 = 792 bp 263 amino acids = 28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| AmpR 11,867 .. 12,727 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 1: signal sequence 12,659 .. 12,727 = 69 bp 23 amino acids = 2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| AmpR 11,867 .. 12,727 = 861 bp 286 amino acids = 31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| WPRE 7552 .. 8140 = 589 bp woodchuck hepatitis virus posttranscriptional regulatory element |
| WPRE 7552 .. 8140 = 589 bp woodchuck hepatitis virus posttranscriptional regulatory element |
| ori 11,108 .. 11,696 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| ori 11,108 .. 11,696 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| f1 ori 9147 .. 9575 = 429 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
| f1 ori 9147 .. 9575 = 429 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
| CMV enhancer 238 .. 617 = 380 bp human cytomegalovirus immediate early enhancer |
| CMV enhancer 238 .. 617 = 380 bp human cytomegalovirus immediate early enhancer |
| BleoR 10,032 .. 10,406 = 375 bp 124 amino acids = 13.8 kDa Product: antibiotic-binding protein confers resistance to bleomycin, phleomycin, and Zeocin™ |
| BleoR 10,032 .. 10,406 = 375 bp 124 amino acids = 13.8 kDa Product: antibiotic-binding protein confers resistance to bleomycin, phleomycin, and Zeocin™ |
| SV40 promoter 9589 .. 9918 = 330 bp SV40 enhancer and early promoter |
| SV40 promoter 9589 .. 9918 = 330 bp SV40 enhancer and early promoter |
| RRE 1680 .. 1913 = 234 bp The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm. |
| RRE 1680 .. 1913 = 234 bp The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm. |
| bGH poly(A) signal 8877 .. 9101 = 225 bp bovine growth hormone polyadenylation signal |
| bGH poly(A) signal 8877 .. 9101 = 225 bp bovine growth hormone polyadenylation signal |
| EF-1α core promoter 2651 .. 2862 = 212 bp core promoter for human elongation factor EF-1α |
| EF-1α core promoter 2651 .. 2862 = 212 bp core promoter for human elongation factor EF-1α |
| CMV promoter 619 .. 817 = 199 bp human cytomegalovirus (CMV) immediate early promoter |
| CMV promoter 619 .. 817 = 199 bp human cytomegalovirus (CMV) immediate early promoter |
| 5' LTR (truncated) 835 .. 1015 = 181 bp truncated 5' long terminal repeat (LTR) from HIV-1 |
| 5' LTR (truncated) 835 .. 1015 = 181 bp truncated 5' long terminal repeat (LTR) from HIV-1 |
| 5' LTR (truncated) 8665 .. 8845 = 181 bp truncated 5' long terminal repeat (LTR) from HIV-1 |
| 5' LTR (truncated) 8665 .. 8845 = 181 bp truncated 5' long terminal repeat (LTR) from HIV-1 |
| HIV-1 Ψ 1062 .. 1187 = 126 bp packaging signal of human immunodeficiency virus type 1 |
| HIV-1 Ψ 1062 .. 1187 = 126 bp packaging signal of human immunodeficiency virus type 1 |
| SV40 poly(A) signal 10,536 .. 10,657 = 122 bp SV40 polyadenylation signal |
| SV40 poly(A) signal 10,536 .. 10,657 = 122 bp SV40 polyadenylation signal |
| cPPT/CTS 2440 .. 2557 = 118 bp central polypurine tract and central termination sequence of HIV-1 |
| cPPT/CTS 2440 .. 2557 = 118 bp central polypurine tract and central termination sequence of HIV-1 |
| AmpR promoter 12,728 .. 12,832 = 105 bp |
| AmpR promoter 12,728 .. 12,832 = 105 bp |
| EM7 promoter 9966 .. 10,013 = 48 bp synthetic bacterial promoter |
| EM7 promoter 9966 .. 10,013 = 48 bp synthetic bacterial promoter |
| SV40 ori 9769 .. 9904 = 136 bp SV40 origin of replication |
| SV40 ori 9769 .. 9904 = 136 bp SV40 origin of replication |
| ORF: 1558 .. 2445 = 888 bp ORF: 295 amino acids = 33.8 kDa |
| ORF: 2887 .. 7527 = 4641 bp ORF: 1546 amino acids = 176.9 kDa |
| ORF: 7642 .. 8691 = 1050 bp ORF: 349 amino acids = 39.4 kDa |
| ORF: 7106 .. 7348 = 243 bp ORF: 80 amino acids = 8.8 kDa |
| ORF: 7655 .. 8167 = 513 bp ORF: 170 amino acids = 18.0 kDa |
| ORF: 9098 .. 9364 = 267 bp ORF: 88 amino acids = 9.3 kDa |
| ORF: 10,032 .. 10,406 = 375 bp ORF: 124 amino acids = 13.8 kDa |
| ORF: 11,997 .. 12,263 = 267 bp ORF: 88 amino acids = 9.2 kDa |
| ORF: 1473 .. 1853 = 381 bp ORF: 126 amino acids = 13.3 kDa |
| ORF: 4236 .. 4529 = 294 bp ORF: 97 amino acids = 10.8 kDa |
| ORF: 4800 .. 5249 = 450 bp ORF: 149 amino acids = 17.3 kDa |
| ORF: 10,025 .. 10,312 = 288 bp ORF: 95 amino acids = 10.1 kDa |
| ORF: 11,867 .. 12,727 = 861 bp ORF: 286 amino acids = 31.6 kDa |
| ORF: 2566 .. 7431 = 4866 bp ORF: 1621 amino acids = 171.1 kDa |
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