Cas9m4-VP64

Plasmid for expression in mammalian cells of catalytically dead Cas9m4 fused to the VP64 transcriptional activation domain.

Sequence Author: Church Lab / Addgene #47319

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CMV enhancer ATG Cas9m4-VP64 9771 bp
VP64
2559 .. 2708  =  150 bp
50 amino acids  =  5.5 kDa
Product: tetrameric repeat of the minimal activation domain of herpes simplex virus VP16 (Beerli et al., 1998)
VP64
2559 .. 2708  =  150 bp
50 amino acids  =  5.5 kDa
Product: tetrameric repeat of the minimal activation domain of herpes simplex virus VP16 (Beerli et al., 1998)
SV40 NLS
2733 .. 2753  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
2733 .. 2753  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
Cas9m4
2766 .. 6869  =  4104 bp
1368 amino acids  =  158.2 kDa
7 segments
   Segment 7:  
   2766 .. 4280  =  1515 bp
   505 amino acids  =  58.3 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the mutations D10A, D839A, H840A, and N863A (Mali et al., 2013)
Cas9m4
2766 .. 6869  =  4104 bp
1368 amino acids  =  158.2 kDa
7 segments
   Segment 6:  
   4281 .. 4283  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the mutations D10A, D839A, H840A, and N863A (Mali et al., 2013)
Cas9m4
2766 .. 6869  =  4104 bp
1368 amino acids  =  158.2 kDa
7 segments
   Segment 5:  
   4284 .. 4349  =  66 bp
   22 amino acids  =  2.5 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the mutations D10A, D839A, H840A, and N863A (Mali et al., 2013)
Cas9m4
2766 .. 6869  =  4104 bp
1368 amino acids  =  158.2 kDa
7 segments
   Segment 4:  
   4350 .. 4355  =  6 bp
   2 amino acids  =  160.2 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the mutations D10A, D839A, H840A, and N863A (Mali et al., 2013)
Cas9m4
2766 .. 6869  =  4104 bp
1368 amino acids  =  158.2 kDa
7 segments
   Segment 3:  
   4356 .. 6839  =  2484 bp
   828 amino acids  =  96.1 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the mutations D10A, D839A, H840A, and N863A (Mali et al., 2013)
Cas9m4
2766 .. 6869  =  4104 bp
1368 amino acids  =  158.2 kDa
7 segments
   Segment 2:  
   6840 .. 6842  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the mutations D10A, D839A, H840A, and N863A (Mali et al., 2013)
Cas9m4
2766 .. 6869  =  4104 bp
1368 amino acids  =  158.2 kDa
7 segments
   Segment 1:  
   6843 .. 6869  =  27 bp
   9 amino acids  =  1.1 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the mutations D10A, D839A, H840A, and N863A (Mali et al., 2013)
Cas9m4
2766 .. 6869  =  4104 bp
1368 amino acids  =  158.2 kDa
7 segments
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the mutations D10A, D839A, H840A, and N863A (Mali et al., 2013)
SV40 NLS
6879 .. 6899  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
6879 .. 6899  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
ATG
6900 .. 6902  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
6900 .. 6902  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
AmpR
7971 .. 8831  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   7971 .. 8039  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
7971 .. 8831  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   8040 .. 8831  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
7971 .. 8831  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
NeoR/KanR
567 .. 1361  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
567 .. 1361  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
ori
9002 .. 9590  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
9002 .. 9590  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
1771 .. 2199  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
1771 .. 2199  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
7221 .. 7600  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
7221 .. 7600  =  380 bp
human cytomegalovirus immediate early enhancer
SV40 promoter
1428 .. 1757  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
1428 .. 1757  =  330 bp
SV40 enhancer and early promoter
CMV promoter
7017 .. 7220  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
7017 .. 7220  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal
270 .. 391  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
270 .. 391  =  122 bp
SV40 polyadenylation signal
AmpR promoter
7866 .. 7970  =  105 bp
AmpR promoter
7866 .. 7970  =  105 bp
HSV TK poly(A) signal
2401 .. 2449  =  49 bp
herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)
HSV TK poly(A) signal
2401 .. 2449  =  49 bp
herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)
SV40 ori
1442 .. 1577  =  136 bp
SV40 origin of replication
SV40 ori
1442 .. 1577  =  136 bp
SV40 origin of replication
ORF:  6727 .. 7002  =  276 bp
ORF:  91 amino acids  =  10.8 kDa
ORF:  798 .. 1052  =  255 bp
ORF:  84 amino acids  =  9.6 kDa
ORF:  2823 .. 3356  =  534 bp
ORF:  177 amino acids  =  20.0 kDa
ORF:  4608 .. 4883  =  276 bp
ORF:  91 amino acids  =  10.3 kDa
ORF:  4947 .. 5465  =  519 bp
ORF:  172 amino acids  =  20.1 kDa
ORF:  6390 .. 6926  =  537 bp
ORF:  178 amino acids  =  19.6 kDa
ORF:  7971 .. 8831  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  567 .. 1361  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  2544 .. 7061  =  4518 bp
ORF:  1505 amino acids  =  174.1 kDa
ORF:  803 .. 1189  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
ORF:  2558 .. 2803  =  246 bp
ORF:  81 amino acids  =  9.1 kDa
ORF:  8435 .. 8701  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
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Download Cas9m4-VP64.dna file

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