LSL-Cas9-Rosa26TV

Zhang lab vector with positive and negative selection markers, for targeting Cre-dependent Cas9 and EGFP expression to the mouse Rosa26 locus.

Sequence Author: Zhang Lab / Addgene #61408

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: CRISPR Plasmids | More Plasmid Sets
No matches
ATG NeoR/KanR Rosa26 left arm chimeric intron loxP SV40 poly(A) signal SV40 poly(A) signal SV40 poly(A) signal loxP ATG 3xFLAG SV40 NLS nucleoplasmin NLS attB LSL-Cas9-Rosa26TV 20,597 bp
ATG
3883 .. 3885  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
3883 .. 3885  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
3xFLAG
3886 .. 3951  =  66 bp
22 amino acids  =  2.7 kDa
Product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site
3xFLAG
3886 .. 3951  =  66 bp
22 amino acids  =  2.7 kDa
Product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site
SV40 NLS
3958 .. 3978  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
3958 .. 3978  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
Cas9
4003 .. 8103  =  4101 bp
1367 amino acids  =  158.3 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
4003 .. 8103  =  4101 bp
1367 amino acids  =  158.3 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
nucleoplasmin NLS
8104 .. 8151  =  48 bp
16 amino acids  =  1.7 kDa
Product: bipartite nuclear localization signal from nucleoplasmin
nucleoplasmin NLS
8104 .. 8151  =  48 bp
16 amino acids  =  1.7 kDa
Product: bipartite nuclear localization signal from nucleoplasmin
P2A
8161 .. 8217  =  57 bp
19 amino acids  =  2.0 kDa
Product: 2A peptide from porcine teschovirus-1 polyprotein
Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.
P2A
8161 .. 8217  =  57 bp
19 amino acids  =  2.0 kDa
Product: 2A peptide from porcine teschovirus-1 polyprotein
Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.
EGFP
8218 .. 8934  =  717 bp
238 amino acids  =  26.8 kDa
2 segments
   Segment 1:  1a  
   8218 .. 8220  =  3 bp
   1 amino acid  =  117.1 Da
Product: enhanced GFP
mammalian codon-optimized
EGFP
8218 .. 8934  =  717 bp
238 amino acids  =  26.8 kDa
2 segments
   Segment 2:  
   8221 .. 8934  =  714 bp
   237 amino acids  =  26.7 kDa
Product: enhanced GFP
mammalian codon-optimized
EGFP
8218 .. 8934  =  717 bp
238 amino acids  =  26.8 kDa
2 segments
Product: enhanced GFP
mammalian codon-optimized
Rosa26 right arm
11,763 .. 16,103  =  4341 bp
right homology arm from intron 1 of the mouse Rosa26 gene (Soriano, 1999)
Rosa26 right arm
11,763 .. 16,103  =  4341 bp
right homology arm from intron 1 of the mouse Rosa26 gene (Soriano, 1999)
Rosa26 left arm
3 .. 1085  =  1083 bp
left homology arm from intron 1 of the mouse Rosa26 gene (Soriano, 1999)
Rosa26 left arm
3 .. 1085  =  1083 bp
left homology arm from intron 1 of the mouse Rosa26 gene (Soriano, 1999)
chimeric intron
1765 .. 2782  =  1018 bp
chimera between introns from chicken β-actin and rabbit β-globin
chimeric intron
1765 .. 2782  =  1018 bp
chimera between introns from chicken β-actin and rabbit β-globin
AmpR
18,949 .. 19,809  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   18,949 .. 19,740  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
18,949 .. 19,809  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   19,741 .. 19,809  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
18,949 .. 19,809  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
NeoR/KanR
10,423 .. 11,226  =  804 bp
267 amino acids  =  29.2 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
10,423 .. 11,226  =  804 bp
267 amino acids  =  29.2 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
ori
18,190 .. 18,778  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
18,190 .. 18,778  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
WPRE
8985 .. 9573  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
WPRE
8985 .. 9573  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
ATG
16,669 .. 16,671  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
16,669 .. 16,671  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
DTA
16,678 .. 17,247  =  570 bp
190 amino acids  =  21.0 kDa
Product: diphteria toxin Fragment A
DTA
16,678 .. 17,247  =  570 bp
190 amino acids  =  21.0 kDa
Product: diphteria toxin Fragment A
PGK promoter
16,152 .. 16,651  =  500 bp
mouse phosphoglycerate kinase 1 promoter
PGK promoter
16,152 .. 16,651  =  500 bp
mouse phosphoglycerate kinase 1 promoter
PGK promoter
9864 .. 10,363  =  500 bp
mouse phosphoglycerate kinase 1 promoter
PGK promoter
9864 .. 10,363  =  500 bp
mouse phosphoglycerate kinase 1 promoter
PGK poly(A) signal
11,236 .. 11,697  =  462 bp
mouse phosphoglycerate kinase 1 polyadenylation signal
PGK poly(A) signal
11,236 .. 11,697  =  462 bp
mouse phosphoglycerate kinase 1 polyadenylation signal
f1 ori
19,940 .. 20,395  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
19,940 .. 20,395  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
1181 .. 1484  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
1181 .. 1484  =  304 bp
human cytomegalovirus immediate early enhancer
chicken β-actin promoter
1486 .. 1763  =  278 bp
chicken β-actin promoter
1486 .. 1763  =  278 bp
bGH poly(A) signal
17,463 .. 17,687  =  225 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
17,463 .. 17,687  =  225 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
9594 .. 9801  =  208 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
9594 .. 9801  =  208 bp
bovine growth hormone polyadenylation signal
SV40 poly(A) signal
3369 .. 3503  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
3369 .. 3503  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
3623 .. 3757  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
3623 .. 3757  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
3115 .. 3249  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
3115 .. 3249  =  135 bp
SV40 polyadenylation signal
AmpR promoter
19,810 .. 19,914  =  105 bp
AmpR promoter
19,810 .. 19,914  =  105 bp
small t intron
17,324 .. 17,389  =  66 bp
simian virus 40 (SV40) small t antigen intron
small t intron
17,324 .. 17,389  =  66 bp
simian virus 40 (SV40) small t antigen intron
loxP
2915 .. 2948  =  34 bp
Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).
loxP
2915 .. 2948  =  34 bp
Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).
loxP
3786 .. 3819  =  34 bp
Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).
loxP
3786 .. 3819  =  34 bp
Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).
attB
9809 .. 9842  =  34 bp
minimal attB site for the φC31 integrase (Groth et al., 2000)
attB
9809 .. 9842  =  34 bp
minimal attB site for the φC31 integrase (Groth et al., 2000)
ORF:  3352 .. 3600  =  249 bp
ORF:  82 amino acids  =  9.2 kDa
ORF:  3883 .. 8934  =  5052 bp
ORF:  1683 amino acids  =  193.6 kDa
ORF:  9088 .. 9606  =  519 bp
ORF:  172 amino acids  =  18.2 kDa
ORF:  10,144 .. 11,226  =  1083 bp
ORF:  360 amino acids  =  39.5 kDa
ORF:  16,432 .. 17,322  =  891 bp
ORF:  296 amino acids  =  32.5 kDa
ORF:  1394 .. 1741  =  348 bp
ORF:  115 amino acids  =  12.1 kDa
ORF:  2594 .. 2842  =  249 bp
ORF:  82 amino acids  =  8.9 kDa
ORF:  3098 .. 3346  =  249 bp
ORF:  82 amino acids  =  9.2 kDa
ORF:  3803 .. 4060  =  258 bp
ORF:  85 amino acids  =  9.8 kDa
ORF:  10,604 .. 10,990  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
ORF:  19,079 .. 19,345  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  1584 .. 1841  =  258 bp
ORF:  85 amino acids  =  8.7 kDa
ORF:  9075 .. 9650  =  576 bp
ORF:  191 amino acids  =  21.0 kDa
ORF:  5349 .. 5642  =  294 bp
ORF:  97 amino acids  =  10.8 kDa
ORF:  5913 .. 6362  =  450 bp
ORF:  149 amino acids  =  17.3 kDa
ORF:  9729 .. 9971  =  243 bp
ORF:  80 amino acids  =  8.5 kDa
ORF:  20,575 .. 559  =  582 bp
ORF:  193 amino acids  =  20.3 kDa
ORF:  1847 .. 2455  =  609 bp
ORF:  202 amino acids  =  21.2 kDa
ORF:  2462 .. 2761  =  300 bp
ORF:  99 amino acids  =  10.5 kDa
ORF:  9536 .. 9793  =  258 bp
ORF:  85 amino acids  =  9.5 kDa
ORF:  15,404 .. 15,649  =  246 bp
ORF:  81 amino acids  =  9.3 kDa
ORF:  430 .. 804  =  375 bp
ORF:  124 amino acids  =  13.9 kDa
ORF:  3781 .. 8946  =  5166 bp
ORF:  1721 amino acids  =  181.0 kDa
ORF:  10,741 .. 10,995  =  255 bp
ORF:  84 amino acids  =  9.6 kDa
ORF:  18,949 .. 19,809  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
Click here to try SnapGene

Download LSL-Cas9-Rosa26TV.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.