M-SPcas

Mammalian cell vector for expressing human codon-optimized Streptococcus pyogenes Cas9 with a G418-resistance marker.

Sequence Author: Church Lab / Addgene #48668

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: CRISPR Plasmids | More Plasmid Sets
No matches
M-SPcas 9553 bp
Cas9
748 .. 4851  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
748 .. 4851  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
SV40 NLS
4864 .. 4884  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
4864 .. 4884  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
AmpR
8372 .. 9232  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   8372 .. 9163  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
8372 .. 9232  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   9164 .. 9232  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
8372 .. 9232  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
NeoR/KanR
6071 .. 6865  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
6071 .. 6865  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
ori
7613 .. 8201  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
7613 .. 8201  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
5233 .. 5661  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
5233 .. 5661  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
50 .. 429  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
50 .. 429  =  380 bp
human cytomegalovirus immediate early enhancer
SV40 promoter
5675 .. 6004  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
5675 .. 6004  =  330 bp
SV40 enhancer and early promoter
CMV promoter
430 .. 633  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
430 .. 633  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal
7041 .. 7162  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
7041 .. 7162  =  122 bp
SV40 polyadenylation signal
AmpR promoter
9233 .. 9337  =  105 bp
AmpR promoter
9233 .. 9337  =  105 bp
HSV TK poly(A) signal
4983 .. 5031  =  49 bp
herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)
HSV TK poly(A) signal
4983 .. 5031  =  49 bp
herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)
SV40 ori
5855 .. 5990  =  136 bp
SV40 origin of replication
SV40 ori
5855 .. 5990  =  136 bp
SV40 origin of replication
ORF:  589 .. 4887  =  4299 bp
ORF:  1432 amino acids  =  166.3 kDa
ORF:  6071 .. 6865  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  6243 .. 6629  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
ORF:  8502 .. 8768  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  6380 .. 6634  =  255 bp
ORF:  84 amino acids  =  9.6 kDa
ORF:  8372 .. 9232  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  724 .. 1227  =  504 bp
ORF:  167 amino acids  =  18.5 kDa
ORF:  2152 .. 2670  =  519 bp
ORF:  172 amino acids  =  20.1 kDa
ORF:  2734 .. 3267  =  534 bp
ORF:  177 amino acids  =  19.9 kDa
ORF:  4261 .. 4794  =  534 bp
ORF:  177 amino acids  =  20.0 kDa
ORF:  648 .. 890  =  243 bp
ORF:  80 amino acids  =  9.6 kDa
Click here to try SnapGene

Download M-SPcas.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.