MLM3639

Plasmid for expressing 3xFLAG®-tagged S. pyogenes Cas9 from either the CMV promoter in vivo or the T7 promoter in vitro.

Sequence Author: Joung Lab / Addgene #42252

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PmeI (4653) SV40 NLS MLM3639 7614 bp
PmeI  (4653)
1 site
G T T T A A A C C A A A T T T G
Cas9
409 .. 4512  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
409 .. 4512  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
SV40 NLS
4519 .. 4539  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
4519 .. 4539  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
3xFLAG
4546 .. 4611  =  66 bp
22 amino acids  =  2.7 kDa
Product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site
3xFLAG
4546 .. 4611  =  66 bp
22 amino acids  =  2.7 kDa
Product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site
AmpR
6131 .. 6991  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   6131 .. 6922  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6131 .. 6991  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   6923 .. 6991  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6131 .. 6991  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
5372 .. 5960  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
5372 .. 5960  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CMV enhancer
7366 .. 131  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
7366 .. 131  =  380 bp
human cytomegalovirus immediate early enhancer
bGH poly(A) signal
4675 .. 4899  =  225 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
4675 .. 4899  =  225 bp
bovine growth hormone polyadenylation signal
CMV promoter
132 .. 335  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
132 .. 335  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
AmpR promoter
6992 .. 7096  =  105 bp
AmpR promoter
6992 .. 7096  =  105 bp
T7 promoter
377 .. 395  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
377 .. 395  =  19 bp
promoter for bacteriophage T7 RNA polymerase
ORF:  409 .. 4623  =  4215 bp
ORF:  1404 amino acids  =  162.5 kDa
ORF:  6261 .. 6527  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  6131 .. 6991  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
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Download MLM3639.dna file

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