PX552

Zhang lab AAV vector, also known as AAV-SpGuide or pAAV SpCas9 acceptor, for sgRNA expression with a nuclear EGFP marker. Use together with PX551.

Sequence Author: Zhang Lab / Addgene #60958

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SapI (401) SapI (422) pol III terminator PX552 5303 bp
SapI  (401)
2 sites
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
SapI  (422)
2 sites
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
EGFP
1017 .. 1733  =  717 bp
239 amino acids  =  26.9 kDa
3 segments
   Segment 1:  
   1017 .. 1019  =  3 bp
   1 amino acid  =  149.2 Da
Product: enhanced GFP
mammalian codon-optimized
EGFP
1017 .. 1733  =  717 bp
239 amino acids  =  26.9 kDa
3 segments
   Segment 2:  1a  
   1020 .. 1022  =  3 bp
   1 amino acid  =  117.1 Da
Product: enhanced GFP
mammalian codon-optimized
EGFP
1017 .. 1733  =  717 bp
239 amino acids  =  26.9 kDa
3 segments
   Segment 3:  
   1023 .. 1733  =  711 bp
   237 amino acids  =  26.7 kDa
Product: enhanced GFP
mammalian codon-optimized
EGFP
1017 .. 1733  =  717 bp
239 amino acids  =  26.9 kDa
3 segments
Product: enhanced GFP
mammalian codon-optimized
KASH
1752 .. 2000  =  249 bp
82 amino acids  =  9.2 kDa
Product: transmembrane KASH domain of nesprin-2
confers localization to the nuclear envelope
KASH
1752 .. 2000  =  249 bp
82 amino acids  =  9.2 kDa
Product: transmembrane KASH domain of nesprin-2
confers localization to the nuclear envelope
AmpR
3623 .. 4483  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   3623 .. 3691  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3623 .. 4483  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   3692 .. 4483  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3623 .. 4483  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
4654 .. 5242  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
4654 .. 5242  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
hGH poly(A) signal
2050 .. 2526  =  477 bp
human growth hormone polyadenylation signal
hGH poly(A) signal
2050 .. 2526  =  477 bp
human growth hormone polyadenylation signal
f1 ori
2781 .. 3236  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
2781 .. 3236  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
hSyn promoter
532 .. 979  =  448 bp
human synapsin I promoter; confers neuron-specific expression (Kügler et al., 2003)
hSyn promoter
532 .. 979  =  448 bp
human synapsin I promoter; confers neuron-specific expression (Kügler et al., 2003)
U6 promoter
156 .. 396  =  241 bp
RNA polymerase III promoter for human U6 snRNA
U6 promoter
156 .. 396  =  241 bp
RNA polymerase III promoter for human U6 snRNA
AAV2 ITR
1 .. 141  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AAV2 ITR
1 .. 141  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AAV2 ITR
2566 .. 2706  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AAV2 ITR
2566 .. 2706  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AmpR promoter
3518 .. 3622  =  105 bp
AmpR promoter
3518 .. 3622  =  105 bp
gRNA scaffold
423 .. 498  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
gRNA scaffold
423 .. 498  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
pol III terminator
499 .. 504  =  6 bp
RNA polymerase III transcription terminator
pol III terminator
499 .. 504  =  6 bp
RNA polymerase III transcription terminator
ORF:  1915 .. 2163  =  249 bp
ORF:  82 amino acids  =  8.5 kDa
ORF:  557 .. 1003  =  447 bp
ORF:  148 amino acids  =  15.4 kDa
ORF:  3623 .. 4483  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  5300 .. 269  =  273 bp
ORF:  90 amino acids  =  10.0 kDa
ORF:  1017 .. 2000  =  984 bp
ORF:  327 amino acids  =  36.8 kDa
ORF:  2364 .. 2804  =  441 bp
ORF:  146 amino acids  =  16.0 kDa
ORF:  2414 .. 2761  =  348 bp
ORF:  115 amino acids  =  13.4 kDa
ORF:  4087 .. 4353  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
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Download PX552.dna file

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