PX851

Zhang lab split-Cas9 plasmid for expressing an N-terminal fragment (amino acids 2-535) of S. pyogenes Cas9 fused to FRB.

Sequence Author: Zhang Lab / Addgene #62883

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: CRISPR Plasmids | More Plasmid Sets
No matches
U6 promoter BbsI (245) BbsI (267) polIII terminator PX851 6181 bp
BbsI  (245)
2 sites
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BbsI  (267)
2 sites
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
ATG
1251 .. 1253  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
1251 .. 1253  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
Cas9(N)
1278 .. 2879  =  1602 bp
534 amino acids  =  61.8 kDa
Product: N-terminal portion of Streptococcus pyogenes Cas9 (Zetsche et al., 2015)
Cas9(N)
1278 .. 2879  =  1602 bp
534 amino acids  =  61.8 kDa
Product: N-terminal portion of Streptococcus pyogenes Cas9 (Zetsche et al., 2015)
FRB
2925 .. 3194  =  270 bp
90 amino acids  =  10.9 kDa
Product: FKBP-rapamycin binding domain of human FRAP
FRB
2925 .. 3194  =  270 bp
90 amino acids  =  10.9 kDa
Product: FKBP-rapamycin binding domain of human FRAP
AmpR
4501 .. 5361  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   4501 .. 4569  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4501 .. 5361  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   4570 .. 5361  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4501 .. 5361  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
5532 .. 6120  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
5532 .. 6120  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
3659 .. 4114  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
3659 .. 4114  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
440 .. 725  =  286 bp
human cytomegalovirus immediate early enhancer; contains an 18-bp deletion relative to the standard CMV enhancer
CMV enhancer
440 .. 725  =  286 bp
human cytomegalovirus immediate early enhancer; contains an 18-bp deletion relative to the standard CMV enhancer
chicken β-actin promoter
727 .. 1004  =  278 bp
chicken β-actin promoter
727 .. 1004  =  278 bp
U6 promoter
1 .. 241  =  241 bp
RNA polymerase III promoter for human U6 snRNA
U6 promoter
1 .. 241  =  241 bp
RNA polymerase III promoter for human U6 snRNA
hybrid intron
1005 .. 1233  =  229 bp
hybrid between chicken β-actin (CBA) and minute virus of mice (MMV) introns (Gray et al., 2011)
hybrid intron
1005 .. 1233  =  229 bp
hybrid between chicken β-actin (CBA) and minute virus of mice (MMV) introns (Gray et al., 2011)
bGH poly(A) signal
3228 .. 3435  =  208 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
3228 .. 3435  =  208 bp
bovine growth hormone polyadenylation signal
AAV2 ITR
3444 .. 3584  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AAV2 ITR
3444 .. 3584  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AmpR promoter
4396 .. 4500  =  105 bp
AmpR promoter
4396 .. 4500  =  105 bp
gRNA scaffold
268 .. 343  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
gRNA scaffold
268 .. 343  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
polIII terminator
344 .. 349  =  6 bp
RNA polymerase III transcription terminator
polIII terminator
344 .. 349  =  6 bp
RNA polymerase III transcription terminator
ORF:  4501 .. 5361  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  635 .. 982  =  348 bp
ORF:  115 amino acids  =  12.1 kDa
ORF:  3458 .. 3682  =  225 bp
ORF:  74 amino acids  =  8.3 kDa
ORF:  825 .. 1136  =  312 bp
ORF:  103 amino acids  =  10.7 kDa
ORF:  1251 .. 3197  =  1947 bp
ORF:  648 amino acids  =  74.6 kDa
ORF:  2624 .. 3022  =  399 bp
ORF:  132 amino acids  =  14.4 kDa
ORF:  3358 .. 3639  =  282 bp
ORF:  93 amino acids  =  10.4 kDa
ORF:  4965 .. 5231  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
Click here to try SnapGene

Download PX851.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.