gRNA_GFP-T2

Plasmid for guide RNA (gRNA) targeting of an integrated GFP gene in human cells using the GFP T2 target sequence.

Sequence Author: Church Lab / Addgene #41820

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polIII terminator gRNA_GFP-T2 3973 bp
NeoR/KanR
1692 .. 2486  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
1692 .. 2486  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
ori
3204 .. 3792  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
3204 .. 3792  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
BleoR
2692 .. 3066  =  375 bp
124 amino acids  =  13.8 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and Zeocin™
BleoR
2692 .. 3066  =  375 bp
124 amino acids  =  13.8 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and Zeocin™
U6 promoter
406 .. 646  =  241 bp
RNA polymerase III promoter for human U6 snRNA
U6 promoter
406 .. 646  =  241 bp
RNA polymerase III promoter for human U6 snRNA
gRNA scaffold
675 .. 750  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
gRNA scaffold
675 .. 750  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
GFP T2
655 .. 674  =  20 bp
GFP gRNA Target 2 sequence
GFP T2
655 .. 674  =  20 bp
GFP gRNA Target 2 sequence
polIII terminator
751 .. 756  =  6 bp
RNA polymerase III transcription terminator
polIII terminator
751 .. 756  =  6 bp
RNA polymerase III transcription terminator
ORF:  217 .. 519  =  303 bp
ORF:  100 amino acids  =  11.0 kDa
ORF:  838 .. 1137  =  300 bp
ORF:  99 amino acids  =  11.1 kDa
ORF:  1864 .. 2250  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
ORF:  2692 .. 3066  =  375 bp
ORF:  124 amino acids  =  13.8 kDa
ORF:  1692 .. 2486  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  2511 .. 3095  =  585 bp
ORF:  194 amino acids  =  21.4 kDa
ORF:  2001 .. 2534  =  534 bp
ORF:  177 amino acids  =  19.9 kDa
ORF:  2664 .. 2972  =  309 bp
ORF:  102 amino acids  =  10.9 kDa
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Download gRNA_GFP-T2.dna file

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Individual Sequences & Maps

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