p415-GalL-Cas9-CYC1t

Yeast centromeric plasmid for expressing human codon-optimized Cas9 with the inducible GAL1 promoter.

Sequence Author: Church Lab / Addgene #43804

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p415-GalL-Cas9-CYC1t 10,793 bp
Cas9
3258 .. 7361  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
3258 .. 7361  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
SV40 NLS
7374 .. 7394  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
7374 .. 7394  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
LEU2
9037 .. 10,131  =  1095 bp
364 amino acids  =  39.0 kDa
Product: 3-isopropylmalate dehydrogenase, required for leucine biosynthesis
yeast auxotrophic marker
LEU2
9037 .. 10,131  =  1095 bp
364 amino acids  =  39.0 kDa
Product: 3-isopropylmalate dehydrogenase, required for leucine biosynthesis
yeast auxotrophic marker
AmpR
715 .. 1575  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   715 .. 783  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
715 .. 1575  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   784 .. 1575  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
715 .. 1575  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
1746 .. 2334  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1746 .. 2334  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CEN/ARS
70 .. 573  =  504 bp
S. cerevisiae CEN6 centromere fused to an autonomously replicating sequence
CEN/ARS
70 .. 573  =  504 bp
S. cerevisiae CEN6 centromere fused to an autonomously replicating sequence
f1 ori
7865 .. 8320  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
7865 .. 8320  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
GAL1 promoter
2794 .. 3220  =  427 bp
inducible promoter, regulated by Gal4
GAL1 promoter
2794 .. 3220  =  427 bp
inducible promoter, regulated by Gal4
LEU2 promoter
8620 .. 9024  =  405 bp
LEU2 promoter
8620 .. 9024  =  405 bp
AmpR promoter
610 .. 714  =  105 bp
AmpR promoter
610 .. 714  =  105 bp
ORF:  715 .. 1575  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  9025 .. 10,131  =  1107 bp
ORF:  368 amino acids  =  39.4 kDa
ORF:  7526 .. 7849  =  324 bp
ORF:  107 amino acids  =  12.0 kDa
ORF:  3258 .. 7397  =  4140 bp
ORF:  1379 amino acids  =  159.7 kDa
ORF:  1179 .. 1445  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  3168 .. 3737  =  570 bp
ORF:  189 amino acids  =  21.2 kDa
ORF:  4662 .. 5180  =  519 bp
ORF:  172 amino acids  =  20.1 kDa
ORF:  5244 .. 5777  =  534 bp
ORF:  177 amino acids  =  19.9 kDa
ORF:  6771 .. 7304  =  534 bp
ORF:  177 amino acids  =  20.0 kDa
ORF:  9699 .. 10,010  =  312 bp
ORF:  103 amino acids  =  11.1 kDa
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