p426-SNR52p-gRNA.CAN1.Y-SUP4t

Yeast high-copy plasmid for gRNA targeting of the negatively selectable CAN1 gene.

Sequence Author: Church Lab / Addgene #43803

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SUP4 terminator CAN1.Y genomic target p426-SNR52p-gRNA.CAN1.Y-SUP4t 6274 bp
2μ ori
69 .. 1411  =  1343 bp
yeast 2μ plasmid origin of replication
2μ ori
69 .. 1411  =  1343 bp
yeast 2μ plasmid origin of replication
AmpR
1543 .. 2403  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   1543 .. 1611  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1543 .. 2403  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   1612 .. 2403  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1543 .. 2403  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
URA3
5055 .. 5858  =  804 bp
267 amino acids  =  29.3 kDa
Product: orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis
yeast auxotrophic marker, counterselectable with 5-fluoroorotic acid (5-FOA)
URA3
5055 .. 5858  =  804 bp
267 amino acids  =  29.3 kDa
Product: orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis
yeast auxotrophic marker, counterselectable with 5-fluoroorotic acid (5-FOA)
ori
2574 .. 3162  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
2574 .. 3162  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
4469 .. 4924  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
4469 .. 4924  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
SNR52 promoter
3621 .. 3889  =  269 bp
promoter for the S. cerevisiae small nucleolar RNA gene SNR52
SNR52 promoter
3621 .. 3889  =  269 bp
promoter for the S. cerevisiae small nucleolar RNA gene SNR52
CYC1 terminator
4014 .. 4261  =  248 bp
transcription terminator for CYC1
CYC1 terminator
4014 .. 4261  =  248 bp
transcription terminator for CYC1
URA3 promoter
5859 .. 6074  =  216 bp
URA3 promoter
5859 .. 6074  =  216 bp
AmpR promoter
1438 .. 1542  =  105 bp
AmpR promoter
1438 .. 1542  =  105 bp
gRNA scaffold
3910 .. 3984  =  75 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
gRNA scaffold
3910 .. 3984  =  75 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
CAN1.Y genomic target
3890 .. 3909  =  20 bp
genomic target sequence located 207 bp downstream of the start codon in the yeast CAN1 gene
CAN1.Y genomic target
3890 .. 3909  =  20 bp
genomic target sequence located 207 bp downstream of the start codon in the yeast CAN1 gene
SUP4 terminator
3989 .. 4008  =  20 bp
transcription terminator for the S. cerevisiae SUP4 tRNA gene
SUP4 terminator
3989 .. 4008  =  20 bp
transcription terminator for the S. cerevisiae SUP4 tRNA gene
ORF:  1543 .. 2403  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  5347 .. 5823  =  477 bp
ORF:  158 amino acids  =  16.8 kDa
ORF:  290 .. 610  =  321 bp
ORF:  106 amino acids  =  12.9 kDa
ORF:  4130 .. 4453  =  324 bp
ORF:  107 amino acids  =  12.0 kDa
ORF:  1086 .. 1376  =  291 bp
ORF:  96 amino acids  =  11.5 kDa
ORF:  2007 .. 2273  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  5055 .. 5858  =  804 bp
ORF:  267 amino acids  =  29.3 kDa
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Download p426-SNR52p-gRNA.CAN1.Y-SUP4t.dna file

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