pAC84-pCR8-dCas9

Gateway® entry vector with a cassette encoding HA epitope-tagged catalytically inactive S. pyogenes dCas9.

Sequence Author: Jaenisch Lab / Addgene #48218

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rrnB T2 terminator ATG pAC84-pCR8-dCas9 7023 bp
ATG
695 .. 697  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
695 .. 697  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
HA
698 .. 724  =  27 bp
9 amino acids  =  1.1 kDa
Product: HA (human influenza hemagglutinin) epitope tag
HA
698 .. 724  =  27 bp
9 amino acids  =  1.1 kDa
Product: HA (human influenza hemagglutinin) epitope tag
SV40 NLS
728 .. 748  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
728 .. 748  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
dCas9
758 .. 4858  =  4101 bp
1367 amino acids  =  158.2 kDa
5 segments
   Segment 1:  
   758 .. 781  =  24 bp
   8 amino acids  =  923.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
758 .. 4858  =  4101 bp
1367 amino acids  =  158.2 kDa
5 segments
   Segment 2:  
   782 .. 784  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
758 .. 4858  =  4101 bp
1367 amino acids  =  158.2 kDa
5 segments
   Segment 3:  
   785 .. 3271  =  2487 bp
   829 amino acids  =  96.3 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
758 .. 4858  =  4101 bp
1367 amino acids  =  158.2 kDa
5 segments
   Segment 4:  
   3272 .. 3274  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
758 .. 4858  =  4101 bp
1367 amino acids  =  158.2 kDa
5 segments
   Segment 5:  
   3275 .. 4858  =  1584 bp
   528 amino acids  =  60.9 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
758 .. 4858  =  4101 bp
1367 amino acids  =  158.2 kDa
5 segments
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
SV40 NLS
4862 .. 4882  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
4862 .. 4882  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SmR
5489 .. 6280  =  792 bp
263 amino acids  =  29.3 kDa
Product: aminoglycoside adenylyltransferase (Murphy, 1985)
confers resistance to spectinomycin and streptomycin
SmR
5489 .. 6280  =  792 bp
263 amino acids  =  29.3 kDa
Product: aminoglycoside adenylyltransferase (Murphy, 1985)
confers resistance to spectinomycin and streptomycin
ori
6373 .. 6961  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
6373 .. 6961  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
attL2
4902 .. 5001  =  100 bp
recombination site for the Gateway® LR reaction
attL2
4902 .. 5001  =  100 bp
recombination site for the Gateway® LR reaction
attL1
569 .. 668  =  100 bp
recombination site for the Gateway® LR reaction
attL1
569 .. 668  =  100 bp
recombination site for the Gateway® LR reaction
rrnB T1 terminator
387 .. 473  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
387 .. 473  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T2 terminator
268 .. 295  =  28 bp
transcription terminator T2 from the E. coli rrnB gene
rrnB T2 terminator
268 .. 295  =  28 bp
transcription terminator T2 from the E. coli rrnB gene
ORF:  628 .. 1086  =  459 bp
ORF:  152 amino acids  =  17.6 kDa
ORF:  641 .. 4894  =  4254 bp
ORF:  1417 amino acids  =  163.8 kDa
ORF:  5270 .. 6280  =  1011 bp
ORF:  336 amino acids  =  37.5 kDa
ORF:  394 .. 696  =  303 bp
ORF:  100 amino acids  =  11.1 kDa
ORF:  2104 .. 2397  =  294 bp
ORF:  97 amino acids  =  10.8 kDa
ORF:  2668 .. 3117  =  450 bp
ORF:  149 amino acids  =  17.3 kDa
ORF:  5521 .. 5778  =  258 bp
ORF:  85 amino acids  =  9.1 kDa
ORF:  5788 .. 6066  =  279 bp
ORF:  92 amino acids  =  9.4 kDa
ORF:  5528 .. 5911  =  384 bp
ORF:  127 amino acids  =  14.6 kDa
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Download pAC84-pCR8-dCas9.dna file

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