pCFD3-dU6-3gRNA

Drosophila expression vector in which a guide RNA (gRNA) is expressed from the U6-3 promoter.

Sequence Author: Bullock Lab / Addgene #49410

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: CRISPR Plasmids | More Plasmid Sets
No matches
BglII (6360) BbsI (423) BbsI (445) polIII terminator XbaI (756) pCFD3-dU6:3gRNA 6365 bp
BglII  (6360)
1 site
A G A T C T T C T A G A
BbsI  (423)
2 sites
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BbsI  (445)
2 sites
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
XbaI  (756)
1 site
T C T A G A A G A T C T
vermilion
3853 .. 5346  =  1494 bp
379 amino acids  =  44.4 kDa
6 segments  =  1140 bp
Product: tryptophan oxygenase
selectable marker, required to synthesize the brown eye pigment in Drosophila
vermilion
3853 .. 5346  =  1494 bp
379 amino acids  =  44.4 kDa
6 segments  =  1140 bp
Product: tryptophan oxygenase
selectable marker, required to synthesize the brown eye pigment in Drosophila
vermilion
3853 .. 5346  =  1494 bp
379 amino acids  =  44.4 kDa
6 segments  =  1140 bp
Product: tryptophan oxygenase
selectable marker, required to synthesize the brown eye pigment in Drosophila
vermilion
3853 .. 5346  =  1494 bp
379 amino acids  =  44.4 kDa
6 segments  =  1140 bp
Product: tryptophan oxygenase
selectable marker, required to synthesize the brown eye pigment in Drosophila
vermilion
3853 .. 5346  =  1494 bp
379 amino acids  =  44.4 kDa
6 segments  =  1140 bp
Product: tryptophan oxygenase
selectable marker, required to synthesize the brown eye pigment in Drosophila
vermilion
3853 .. 5346  =  1494 bp
379 amino acids  =  44.4 kDa
6 segments  =  1140 bp
   Segment 6:  
   3853 .. 3970  =  118 bp
   38 amino acids  =  4.5 kDa
Product: tryptophan oxygenase
selectable marker, required to synthesize the brown eye pigment in Drosophila
vermilion
3853 .. 5346  =  1494 bp
379 amino acids  =  44.4 kDa
6 segments  =  1140 bp
   Segment 5:  
   4047 .. 4140  =  94 bp
   32 amino acids  =  3.5 kDa
Product: tryptophan oxygenase
selectable marker, required to synthesize the brown eye pigment in Drosophila
vermilion
3853 .. 5346  =  1494 bp
379 amino acids  =  44.4 kDa
6 segments  =  1140 bp
   Segment 4:  
   4232 .. 4838  =  607 bp
   202 amino acids  =  24.0 kDa
Product: tryptophan oxygenase
selectable marker, required to synthesize the brown eye pigment in Drosophila
vermilion
3853 .. 5346  =  1494 bp
379 amino acids  =  44.4 kDa
6 segments  =  1140 bp
   Segment 3:  
   4893 .. 5026  =  134 bp
   45 amino acids  =  5.5 kDa
Product: tryptophan oxygenase
selectable marker, required to synthesize the brown eye pigment in Drosophila
vermilion
3853 .. 5346  =  1494 bp
379 amino acids  =  44.4 kDa
6 segments  =  1140 bp
   Segment 2:  
   5087 .. 5247  =  161 bp
   53 amino acids  =  6.1 kDa
Product: tryptophan oxygenase
selectable marker, required to synthesize the brown eye pigment in Drosophila
vermilion
3853 .. 5346  =  1494 bp
379 amino acids  =  44.4 kDa
6 segments  =  1140 bp
   Segment 1:  
   5321 .. 5346  =  26 bp
   9 amino acids  =  899.0 Da
Product: tryptophan oxygenase
selectable marker, required to synthesize the brown eye pigment in Drosophila
vermilion
3853 .. 5346  =  1494 bp
379 amino acids  =  44.4 kDa
6 segments  =  1140 bp
Product: tryptophan oxygenase
selectable marker, required to synthesize the brown eye pigment in Drosophila
AmpR
2032 .. 2892  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   2032 .. 2823  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2032 .. 2892  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   2824 .. 2892  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2032 .. 2892  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
1273 .. 1861  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1273 .. 1861  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
3023 .. 3478  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
3023 .. 3478  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
dU6-3 promoter
7 .. 427  =  421 bp
RNA polymerase III promoter for Drosophila U6-3 snRNA (Port et al., 2014)
dU6-3 promoter
7 .. 427  =  421 bp
RNA polymerase III promoter for Drosophila U6-3 snRNA (Port et al., 2014)
gypsy insulator
5973 .. 6358  =  386 bp
chromatin insulator from Drosophila
gypsy insulator
5973 .. 6358  =  386 bp
chromatin insulator from Drosophila
AmpR promoter
2893 .. 2997  =  105 bp
AmpR promoter
2893 .. 2997  =  105 bp
gRNA scaffold
446 .. 521  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
gRNA scaffold
446 .. 521  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
attB
5661 .. 5730  =  70 bp
attB site for the φC31 integrase (Groth et al., 2000)
attB
5661 .. 5730  =  70 bp
attB site for the φC31 integrase (Groth et al., 2000)
polIII terminator
522 .. 527  =  6 bp
RNA polymerase III transcription terminator
polIII terminator
522 .. 527  =  6 bp
RNA polymerase III transcription terminator
ORF:  2162 .. 2428  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  4350 .. 4598  =  249 bp
ORF:  82 amino acids  =  9.0 kDa
ORF:  4212 .. 4805  =  594 bp
ORF:  197 amino acids  =  23.5 kDa
ORF:  5079 .. 5333  =  255 bp
ORF:  84 amino acids  =  9.7 kDa
ORF:  5964 .. 6344  =  381 bp
ORF:  126 amino acids  =  15.2 kDa
ORF:  2032 .. 2892  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  5722 .. 6045  =  324 bp
ORF:  107 amino acids  =  12.2 kDa
Click here to try SnapGene

Download pCFD3-dU6-3gRNA.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.