pCas-Guide-AAVS1

Mammalian guide RNA (gRNA) vector for transgene integration at the AAVS1 locus in the human genome. Use together with pAAVS1-Puro-DNR.

Sequence Author: OriGene

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SV40 NLS SV40 NLS polIII terminator CMV enhancer pCas-Guide-AAVS1 8009 bp
Cas9
1173 .. 5276  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
1173 .. 5276  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
SV40 NLS
5277 .. 5297  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
5277 .. 5297  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
5304 .. 5324  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
5304 .. 5324  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
Myc
5346 .. 5375  =  30 bp
10 amino acids  =  1.2 kDa
Product: Myc (human c-Myc oncogene) epitope tag
Myc
5346 .. 5375  =  30 bp
10 amino acids  =  1.2 kDa
Product: Myc (human c-Myc oncogene) epitope tag
FLAG
5394 .. 5417  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
FLAG
5394 .. 5417  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
AmpR
6970 .. 7830  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   6970 .. 7761  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6970 .. 7830  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   7762 .. 7830  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6970 .. 7830  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
hGH poly(A) signal
5464 .. 6086  =  623 bp
human growth hormone polyadenylation signal
hGH poly(A) signal
5464 .. 6086  =  623 bp
human growth hormone polyadenylation signal
ori
6207 .. 6795  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
6207 .. 6795  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CMV enhancer
487 .. 866  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
487 .. 866  =  380 bp
human cytomegalovirus immediate early enhancer
U6 promoter
90 .. 330  =  241 bp
RNA polymerase III promoter for human U6 snRNA
U6 promoter
90 .. 330  =  241 bp
RNA polymerase III promoter for human U6 snRNA
CMV promoter
867 .. 1070  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
867 .. 1070  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
gRNA scaffold
360 .. 435  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
gRNA scaffold
360 .. 435  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
AAVS1 target sequence
340 .. 359  =  20 bp
AAVS1 target sequence
340 .. 359  =  20 bp
polIII terminator
436 .. 441  =  6 bp
RNA polymerase III transcription terminator
polIII terminator
436 .. 441  =  6 bp
RNA polymerase III transcription terminator
ORF:  6176 .. 6532  =  357 bp
ORF:  118 amino acids  =  13.2 kDa
ORF:  7100 .. 7366  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  1026 .. 5423  =  4398 bp
ORF:  1465 amino acids  =  169.8 kDa
ORF:  1431 .. 1673  =  243 bp
ORF:  80 amino acids  =  9.6 kDa
ORF:  3225 .. 3569  =  345 bp
ORF:  114 amino acids  =  13.5 kDa
ORF:  3723 .. 4127  =  405 bp
ORF:  134 amino acids  =  15.6 kDa
ORF:  4596 .. 5219  =  624 bp
ORF:  207 amino acids  =  24.3 kDa
ORF:  5828 .. 6052  =  225 bp
ORF:  74 amino acids  =  8.1 kDa
ORF:  6970 .. 7830  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
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Download pCas-Guide-AAVS1.dna file

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Individual Sequences & Maps

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