pGuide-it-ZsGreen1 Vector (Linear)

Linearized CRISPR/Cas9 vector with a ZsGreen1 marker, for cloning and expression of a single guide RNA (sgRNA).

Sequence Author: Clontech (TaKaRa)

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Guide-it™ Sequencing Primer 1 (8224 .. 8247) FLAG SV40 NLS polIII terminator pGuide-it-ZsGreen1 Vector (Linear) 8303 bp
Guide-it™ Sequencing Primer 1
24-mer  /  38% GC
1 binding site
8224 .. 8247  =  24 annealed bases
Tm  =  56°C
Cas9
3551 .. 7654  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
3551 .. 7654  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
SV40 NLS
7670 .. 7690  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
7670 .. 7690  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
FLAG
7691 .. 7714  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
FLAG
7691 .. 7714  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
AmpR
908 .. 1768  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 2:  
   908 .. 1699  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
908 .. 1768  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 1:  signal sequence  
   1700 .. 1768  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
908 .. 1768  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ZsGreen1
2126 .. 2821  =  696 bp
231 amino acids  =  26.1 kDa
Product: Zoanthus green fluorescent protein
mammalian codon-optimized
ZsGreen1
2126 .. 2821  =  696 bp
231 amino acids  =  26.1 kDa
Product: Zoanthus green fluorescent protein
mammalian codon-optimized
ori
149 .. 737  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
149 .. 737  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CMV enhancer
2974 .. 3277  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
2974 .. 3277  =  304 bp
human cytomegalovirus immediate early enhancer
U6 promoter
8057 .. 8297  =  241 bp
RNA polymerase III promoter for human U6 snRNA
U6 promoter
8057 .. 8297  =  241 bp
RNA polymerase III promoter for human U6 snRNA
CMV promoter
3278 .. 3481  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
3278 .. 3481  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
AmpR promoter
1769 .. 1873  =  105 bp
AmpR promoter
1769 .. 1873  =  105 bp
gRNA scaffold
1 .. 86  =  86 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
gRNA scaffold
1 .. 86  =  86 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
SV40 poly(A) signal
1906 .. 1987  =  82 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1906 .. 1987  =  82 bp
SV40 polyadenylation signal
SV40 poly(A) signal
7855 .. 7936  =  82 bp
SV40 polyadenylation signal
SV40 poly(A) signal
7855 .. 7936  =  82 bp
SV40 polyadenylation signal
minimal CMV promoter
2862 .. 2899  =  38 bp
human cytomegalovirus (CMV) immediate early promoter
minimal CMV promoter
2862 .. 2899  =  38 bp
human cytomegalovirus (CMV) immediate early promoter
polIII terminator
87 .. 92  =  6 bp
RNA polymerase III transcription terminator
polIII terminator
87 .. 92  =  6 bp
RNA polymerase III transcription terminator
ORF:  2078 .. 2923  =  846 bp
ORF:  281 amino acids  =  27.9 kDa
ORF:  3551 .. 7717  =  4167 bp
ORF:  1388 amino acids  =  160.7 kDa
ORF:  1038 .. 1304  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  2151 .. 2444  =  294 bp
ORF:  97 amino acids  =  10.9 kDa
ORF:  4900 .. 5193  =  294 bp
ORF:  97 amino acids  =  10.9 kDa
ORF:  5515 .. 5913  =  399 bp
ORF:  132 amino acids  =  15.6 kDa
ORF:  2109 .. 2906  =  798 bp
ORF:  265 amino acids  =  31.2 kDa
ORF:  908 .. 1768  =  861 bp
ORF:  286 amino acids  =  31.5 kDa
ORF:  2126 .. 2821  =  696 bp
ORF:  231 amino acids  =  26.1 kDa
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Download pGuide-it-ZsGreen1 Vector (Linear).dna file

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