CRISPR Plasmids
CRISPR plasmids for genome editing and gene regulation from Addgene, transOMIC, and others
Updated December 20th, 2018
These combined DNA sequence and map files can be opened with 
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CRISPR Plasmids.zip (9 MB)
283 Sequences
283 Sequences
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- Basic Cloning Vectors
- CRISPR Plasmids
- Gateway® Cloning Vectors
- TA and GC Cloning Vectors
- Fluorescent Protein Genes & Plasmids
- Luciferase Vectors
- Mammalian Expression Vectors
- Viral Expression & Packaging Vectors
- I.M.A.G.E. Consortium Plasmids
- Yeast Plasmids
- Plant Vectors
- Insect Cell Vectors
- Lucigen Vectors
- pET & Duet Vectors (Novagen)
- pGEX Vectors (GE Healthcare)
- Qiagen Vectors
- Structural Genomics Vectors
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Individual Sequences & Maps
- 3xFLAG-dCas9/pCMV-7.1
- 3xFLAG-dCas9/pMXs-neo
- 3xFLAG-dCas9/pMXs-puro
- Cas9m4
- Cas9m4-VP64
- CRISPRi library backbone
- dCAS9-VP64_GFP
- eSpCas9(1.1)
- GeneArt CRISPR Nuclease Vector - CD4 (linearized)
- GeneArt CRISPR Nuclease Vector - OFP (linearized)
- gRNA_AAVS1-T1
- gRNA_AAVS1-T2
- gRNA_Cloning Vector
- gRNA_GFP-T1
- gRNA_GFP-T2
- HBT-pcoCas9
- hCas9
- hCas9_D10A
- JDS246
- lentiCas9-Blast
- lentiCas9-EGFP
- lentiCas9n(D10A)-Blast
- lentiCRISPR v2
- lentiGuide-Puro
- lenti dCAS-VP64_Blast
- lenti MS2-P65-HSF1_Hygro
- lenti sgRNA(MS2)_zeo backbone
- LSL-Cas9-Rosa26TV
- M-SPcas
- M-ST1cas
- MLM3613
- MLM3636
- MLM3639
- MS2-P65-HSF1_GFP
- MSP469
- MSP1101
- MSP2133
- MSP2135
- p3s-Cas9HC
- p414-TEF1p-Cas9-CYC1t
- p415-GalL-Cas9-CYC1t
- p426-SNR52p-gRNA.CAN1.Y-SUP4t
- pAAV-Guide-it-1 Vector (Linear)
- pAAV-Guide-it-Down Vector
- pAAV-Guide-it-Up Vector
- pAAVS1-BSD-DNR
- pAAVS1-Puro-DNR
- pAc-sgRNA-Cas9
- pAC2-dual-dCas9VP48-sgExpression
- pAC84-pCR8-dCas9
- pAC91-pmax-dCas9VP64
- pAC93-pmax-dCas9VP160
- pAC94-pmax-dCas9VP160-2A-puro
- pAC95-pmax-dCas9VP160-2A-neo
- pAC149-pCR8-dCas9VP160
- pAC152-dual-dCas9VP64-sgExpression
- pAC153-dual-dCas9VP96-sgExpression
- pAC154-dual-dCas9VP160-sgExpression
- pAC155-pCR8-sgExpression
- pACT2-CAS9c
- pBS-Hsp70-Cas9
- pCAG-T3-hCAS-pA
- pCAG-T3-hCasD10A-pA
- pCas
- pCas-Guide
- pCas-Guide-AAVS1
- pCas-Guide-EF1a-CD4
- pCas-Guide-EF1a-GFP
- pCas-Guide-Nickase
- pCas-Guide-ROSA26
- pCas9
- pCas9D10A_GFP
- pCas9_GFP
- pcDNA-dCas9
- pcDNA-dCas9-p300 Core
- pcDNA-dCas9-p300 Core (D1399Y)
- pcDNA-dCas9-VP64
- pcDNA3.1-CibN-dCas9-CibN
- pCFD1-dU6:1gRNA
- pCFD2-dU6:2gRNA
- pCFD3-dU6:3gRNA
- pCFD4-U6:1_U6:3tandemgRNAs
- pCLIP-All-EFS-Ametrine
- pCLIP-All-EFS-Blast
- pCLIP-All-EFS-Puro
- pCLIP-All-EFS-tRFP
- pCLIP-All-EFS-ZsGreen
- pCLIP-All-hCMV-Blast
- pCLIP-All-hCMV-Puro
- pCLIP-All-hCMV-tRFP
- pCLIP-All-hCMV-ZsGreen
- pCLIP-All-Nickase(D10A)-EFS-Blast
- pCLIP-All-Nickase(D10A)-EFS-Puro
- pCLIP-All-Nickase(D10A)-EFS-tRFP
- pCLIP-All-Nickase(D10A)-EFS-ZsGreen
- pCLIP-All-Nickase(D10A)-hCMV-Puro
- pCLIP-All-Nickase(D10A)-hCMV-tRFP
- pCLIP-All-Nickase(D10A)-hCMV-ZsGreen
- pCLIP-All-Nickase(D10A)-TRE3G-Blast
- pCLIP-All-Nickase(D10A)-TRE3G-Puro
- pCLIP-All-Nickase(D10A)-TRE3G-tRFP
- pCLIP-All-Nickase(D10A)-TRE3G-ZsGreen
- pCLIP-All-TRE3G-Blast
- pCLIP-All-TRE3G-Puro
- pCLIP-All-TRE3G-tRFP
- pCLIP-All-TRE3G-ZsGreen
- pCLIP-Cas9-Nickase-EFS-Blast
- pCLIP-Cas9(D10a)-Nickase-EFS-Puro
- pCLIP-Cas9-Nickase-EFS-tRFP
- pCLIP-Cas9-Nickase-EFS-ZsGreen
- pCLIP-Cas9-Nickase-hCMV-Blast
- pCLIP-Cas9-Nickase-hCMV-Puro
- pCLIP-Cas9-Nickase-hCMV-tRFP
- pCLIP-Cas9-Nickase-hCMV-ZsGreen
- pCLIP-Cas9-Nickase-TRE3G-Blast
- pCLIP-Cas9-Nickase-TRE3G-Puro
- pCLIP-Cas9-Nickase-TRE3G-tRFP
- pCLIP-Cas9-Nickase-TRE3G-ZsGreen
- pCLIP-Cas9-Nuclease-EFS-Blast
- pCLIP-Cas9-Nuclease-EFS-Puro
- pCLIP-Cas9-Nuclease-EFS-tRFP
- pCLIP-Cas9-Nuclease-EFS-ZsGreen
- pCLIP-Cas9-Nuclease-hCMV-Blast
- pCLIP-Cas9-Nuclease-hCMV-Puro
- pCLIP-Cas9-Nuclease-hCMV-tRFP
- pCLIP-Cas9-Nuclease-hCMV-ZsGreen
- pCLIP-Cas9-Nuclease-TRE3G-Blast
- pCLIP-Cas9-Nuclease-TRE3G-Puro
- pCLIP-Cas9-Nuclease-TRE3G-tRFP
- pCLIP-Cas9-Nuclease-TRE3G-ZsGreen
- pCLIP-gRNA-EFS-Blast
- pCLIP-gRNA-EFS-Puro
- pCLIP-gRNA-EFS-tRFP
- pCLIP-gRNA-EFS-ZsGreen
- pCLIP-gRNA-hCMV-Blast
- pCLIP-gRNA-hCMV-Puro
- pCLIP-gRNA-hCMV-tRFP
- pCLIP-gRNA-hCMV-ZsGreen
- pCRCT
- pCRISPomyces-1
- pCRISPomyces-2
- pCRISPR
- pCS2+hSpCas9
- pCS2-Cas9
- pCS2-nCas9n
- pCW-Cas9
- pdCas9
- pdCas9-bacteria
- pdCas9-humanized
- pdCas9::BFP-humanized
- pDCC6
- pDD162 (Peft-3::Cas9 + Empty sgRNA)
- pDR274
- Peft-3::cas9-SV40_NLS::tbb-2 3'UTR
- pET-28b-Cas9-His
- pET-Cas9-6xHis
- pET-Cas9-NLS-6xHis
- pET-NLS-Cas9-6xHis
- pET28a/Cas9-Cys
- pFGC-pcoCas9
- pGL3-U6-sgRNA-PGK-puromycin
- pgRNA-bacteria
- pgRNA-humanized
- pGuide
- pGuide-it-sgRNA1 Vector (Linear)
- pGuide-it-tdTomato Vector (Linear)
- pGuide-it-ZsGreen1 Vector (Linear)
- pHAGE-TO-dCas9-3XGFP
- pHAGE-TO-dCas9-3XmCherry
- pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2
- pHAGE-TO-nmdCas9-3XGFP
- pHAGE-TO-nmdCas9-3XmCherry
- pHAGE EF1α dCas9-KRAB
- pHAGE EF1α dCas9-VP64
- pHAGE TRE dCas9
- pHAGE TRE dCas9-KRAB
- pHAGE TRE dCas9-VP64
- pHR-SFFV-dCas9-BFP
- pHR-SFFV-dCas9-BFP-KRAB
- pHR-SFFV-KRAB-dCas9-P2A-mCherry
- pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP
- pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE
- pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS
- pHSN401
- pHsp70-Cas9
- pICH41308::hCas9
- pICH47742::2x35S-5'UTR-hCas9(STOP)-NOST
- piCRg Entry
- pICSL01009::AtU6p
- pK7WGF2::hCas9
- pKLV-U6gRNA(BbsI)-PGKpuro2ABFP
- pL-CRISPR.EFS.GFP
- pL-CRISPR.EFS.tRFP
- pL-CRISPR.SFFV.GFP
- pL-CRISPR.SFFV.tRFP
- pLenti-Cas-Guide
- pLenti-Cas9
- pLenti-Cas9-IRES-Puro
- pLenti-Cas9-P2A-tGFP
- pLenti-EF1a-Cas9-IRES-Puro
- pLenti-Guide-Puro
- pLKO.1-puro U6 sgRNA BfuAI stuffer
- pLSC-5
- pLVX-hyg-sgRNA1 Vector (Linear)
- pLVX-puro-Cas9
- pLV hUbC-Cas9-T2A-GFP
- pLV hUbC-dCas9-T2A-GFP
- pLV hUbC-dCas9 VP64-T2A-GFP
- pLX-sgRNA
- pMB66
- pMB70
- pME-Cas9
- pME-Cas9-T2A-GFP
- pMJ806
- pMJ841
- pMJ920
- pMLM3705
- pMSCV-LTR-dCas9-p65AD-BFP
- pMSCV-LTR-dCas9-VP64-BFP
- pMZ374
- pNW3
- pRGE31
- pRGEB31
- pRGEB32
- pROSA26-Puro-DNR
- pRPR1_gRNA_handle_RPR1t
- pSECC
- pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)
- pSLQ1658-dCas9-EGFP
- pSpCas9(BB)-2A-GFP (PX458)
- pSpCas9(BB)-2A-Puro (PX459) V2.0
- pSpCas9n(BB)-2A-GFP (PX461)
- pSpCas9n(BB)-2A-Puro (PX462) V2.0
- pSpCas9n(BB) (PX460)
- pSpCas9 (PX165)
- pSPgRNA
- pSQT834
- pSQT1313
- pST1374-N-NLS-flag-linker-Cas9-D10A
- pST1374-N-NLS-flag-linker-Cas9-H840A
- pST1374-NLS-flag-linker-Cas9
- pT3TS-nCas9n
- pT7-Cas9
- pT7-Cas9-Nickase
- pT7-gRNA
- pT7-Guide-IVT
- pTDH3-dCas9
- pTDH3-dCas9-Mxi1
- pTOL-hCMV-TET3G-Hygro Transactivator
- pTPGI_dCas9_VP64
- pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B
- pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E4orf6
- pU6-BbsI-chiRNA
- PU6::klp-12_sgRNA
- pUC57-sgRNA expression vector
- pUC119-gRNA
- Puro-Cas9 donor
- pWJ66
- pWJ68
- pwtCas9-bacteria
- pX260
- pX330
- pX330A-1x2
- pX330S-2
- pX334
- pX335
- PX551
- PX552
- pX600
- pX601
- pX602
- PX851
- PX852
- PX853
- PX854
- PX855
- PX856
- pY010 (pcDNA3.1-hAsCpf1)
- pY016 (pcDNA3.1-hLbCpf1)
- sgRNA(MS2) cloning backbone
- SP-Cas9
- SP-dCas9-VPR
- VP12
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