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CRISPR Plasmids
CRISPR plasmids for genome editing and gene regulation from Addgene, transOMIC, and others
Updated November 20th, 2020
These combined DNA sequence and map files can be opened with 
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CRISPR Plasmids.zip (9 MB)
286 Sequences
286 Sequences
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CRISPR Plasmids
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Individual Sequences & Maps
- 3xFLAG-dCas9/pCMV-7.1
- 3xFLAG-dCas9/pMXs-neo
- 3xFLAG-dCas9/pMXs-puro
- Cas9m4
- Cas9m4-VP64
- CRISPRi library backbone
- dCAS9-VP64_GFP
- eSpCas9(1.1)
- GeneArt CRISPR Nuclease Vector - CD4 (linearized)
- GeneArt CRISPR Nuclease Vector - OFP (linearized)
- gRNA_AAVS1-T1
- gRNA_AAVS1-T2
- gRNA_Cloning Vector
- gRNA_GFP-T1
- gRNA_GFP-T2
- HBT-pcoCas9
- hCas9
- hCas9_D10A
- JDS246
- lentiCas9-Blast
- lentiCas9-EGFP
- lentiCas9n(D10A)-Blast
- lentiCRISPR v2
- lentiGuide-Puro
- lenti dCAS-VP64_Blast
- lenti MS2-P65-HSF1_Hygro
- lenti sgRNA(MS2)_zeo backbone
- LSL-Cas9-Rosa26TV
- M-SPcas
- M-ST1cas
- MLM3613
- MLM3636
- MLM3639
- MS2-P65-HSF1_GFP
- MSP469
- MSP1101
- MSP2133
- MSP2135
- p3s-Cas9HC
- p414-TEF1p-Cas9-CYC1t
- p415-GalL-Cas9-CYC1t
- p426-SNR52p-gRNA.CAN1.Y-SUP4t
- pAAV-Guide-it-1 Vector (Linear)
- pAAV-Guide-it-Down Vector
- pAAV-Guide-it-Up Vector
- pAAVS1-BSD-DNR
- pAAVS1-Puro-DNR
- pAc-sgRNA-Cas9
- pAC2-dual-dCas9VP48-sgExpression
- pAC84-pCR8-dCas9
- pAC91-pmax-dCas9VP64
- pAC93-pmax-dCas9VP160
- pAC94-pmax-dCas9VP160-2A-puro
- pAC95-pmax-dCas9VP160-2A-neo
- pAC149-pCR8-dCas9VP160
- pAC152-dual-dCas9VP64-sgExpression
- pAC153-dual-dCas9VP96-sgExpression
- pAC154-dual-dCas9VP160-sgExpression
- pAC155-pCR8-sgExpression
- pACT2-CAS9c
- pBS-Hsp70-Cas9
- pCAG-T3-hCAS-pA
- pCAG-T3-hCasD10A-pA
- pCas
- pCas-Guide
- pCas-Guide-AAVS1
- pCas-Guide-CRISPRa
- pCas-Guide-CRISPRi
- pCas-Guide-EF1a-CD4
- pCas-Guide-EF1a-GFP
- pCas-Guide-Nickase
- pCas-Guide-ROSA26
- pCas9
- pCas9D10A_GFP
- pCas9_GFP
- pcDNA-dCas9
- pcDNA-dCas9-p300 Core
- pcDNA-dCas9-p300 Core (D1399Y)
- pcDNA-dCas9-VP64
- pcDNA3.1-CibN-dCas9-CibN
- pCFD1-dU6:1gRNA
- pCFD2-dU6:2gRNA
- pCFD3-dU6:3gRNA
- pCFD4-U6:1_U6:3tandemgRNAs
- pCLIP-All-EFS-Ametrine
- pCLIP-All-EFS-Blast
- pCLIP-All-EFS-Puro
- pCLIP-All-EFS-tRFP
- pCLIP-All-EFS-ZsGreen
- pCLIP-All-hCMV-Blast
- pCLIP-All-hCMV-Puro
- pCLIP-All-hCMV-tRFP
- pCLIP-All-hCMV-ZsGreen
- pCLIP-All-Nickase(D10A)-EFS-Blast
- pCLIP-All-Nickase(D10A)-EFS-Puro
- pCLIP-All-Nickase(D10A)-EFS-tRFP
- pCLIP-All-Nickase(D10A)-EFS-ZsGreen
- pCLIP-All-Nickase(D10A)-hCMV-Puro
- pCLIP-All-Nickase(D10A)-hCMV-tRFP
- pCLIP-All-Nickase(D10A)-hCMV-ZsGreen
- pCLIP-All-Nickase(D10A)-TRE3G-Blast
- pCLIP-All-Nickase(D10A)-TRE3G-Puro
- pCLIP-All-Nickase(D10A)-TRE3G-tRFP
- pCLIP-All-Nickase(D10A)-TRE3G-ZsGreen
- pCLIP-All-TRE3G-Blast
- pCLIP-All-TRE3G-Puro
- pCLIP-All-TRE3G-tRFP
- pCLIP-All-TRE3G-ZsGreen
- pCLIP-Cas9-Nickase-EFS-Blast
- pCLIP-Cas9(D10a)-Nickase-EFS-Puro
- pCLIP-Cas9-Nickase-EFS-tRFP
- pCLIP-Cas9-Nickase-EFS-ZsGreen
- pCLIP-Cas9-Nickase-hCMV-Blast
- pCLIP-Cas9-Nickase-hCMV-Puro
- pCLIP-Cas9-Nickase-hCMV-tRFP
- pCLIP-Cas9-Nickase-hCMV-ZsGreen
- pCLIP-Cas9-Nickase-TRE3G-Blast
- pCLIP-Cas9-Nickase-TRE3G-Puro
- pCLIP-Cas9-Nickase-TRE3G-tRFP
- pCLIP-Cas9-Nickase-TRE3G-ZsGreen
- pCLIP-Cas9-Nuclease-EFS-Blast
- pCLIP-Cas9-Nuclease-EFS-Puro
- pCLIP-Cas9-Nuclease-EFS-tRFP
- pCLIP-Cas9-Nuclease-EFS-ZsGreen
- pCLIP-Cas9-Nuclease-hCMV-Blast
- pCLIP-Cas9-Nuclease-hCMV-Puro
- pCLIP-Cas9-Nuclease-hCMV-tRFP
- pCLIP-Cas9-Nuclease-hCMV-ZsGreen
- pCLIP-Cas9-Nuclease-TRE3G-Blast
- pCLIP-Cas9-Nuclease-TRE3G-Puro
- pCLIP-Cas9-Nuclease-TRE3G-tRFP
- pCLIP-Cas9-Nuclease-TRE3G-ZsGreen
- pCLIP-gRNA-EFS-Blast
- pCLIP-gRNA-EFS-Puro
- pCLIP-gRNA-EFS-tRFP
- pCLIP-gRNA-EFS-ZsGreen
- pCLIP-gRNA-hCMV-Blast
- pCLIP-gRNA-hCMV-Puro
- pCLIP-gRNA-hCMV-tRFP
- pCLIP-gRNA-hCMV-ZsGreen
- pCRCT
- pCRISPomyces-1
- pCRISPomyces-2
- pCRISPR
- pCRISPRa-Enhancer
- pCS2+hSpCas9
- pCS2-Cas9
- pCS2-nCas9n
- pCW-Cas9
- pdCas9
- pdCas9-bacteria
- pdCas9-humanized
- pdCas9::BFP-humanized
- pDCC6
- pDD162 (Peft-3::Cas9 + Empty sgRNA)
- pDR274
- Peft-3::cas9-SV40_NLS::tbb-2 3'UTR
- pET-28b-Cas9-His
- pET-Cas9-6xHis
- pET-Cas9-NLS-6xHis
- pET-NLS-Cas9-6xHis
- pET28a/Cas9-Cys
- pFGC-pcoCas9
- pGL3-U6-sgRNA-PGK-puromycin
- pgRNA-bacteria
- pgRNA-humanized
- pGuide
- pGuide-it-sgRNA1 Vector (Linear)
- pGuide-it-tdTomato Vector (Linear)
- pGuide-it-ZsGreen1 Vector (Linear)
- pHAGE-TO-dCas9-3XGFP
- pHAGE-TO-dCas9-3XmCherry
- pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2
- pHAGE-TO-nmdCas9-3XGFP
- pHAGE-TO-nmdCas9-3XmCherry
- pHAGE EF1α dCas9-KRAB
- pHAGE EF1α dCas9-VP64
- pHAGE TRE dCas9
- pHAGE TRE dCas9-KRAB
- pHAGE TRE dCas9-VP64
- pHR-SFFV-dCas9-BFP
- pHR-SFFV-dCas9-BFP-KRAB
- pHR-SFFV-KRAB-dCas9-P2A-mCherry
- pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP
- pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE
- pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS
- pHSN401
- pHsp70-Cas9
- pICH41308::hCas9
- pICH47742::2x35S-5'UTR-hCas9(STOP)-NOST
- piCRg Entry
- pICSL01009::AtU6p
- pK7WGF2::hCas9
- pKLV-U6gRNA(BbsI)-PGKpuro2ABFP
- pL-CRISPR.EFS.GFP
- pL-CRISPR.EFS.tRFP
- pL-CRISPR.SFFV.GFP
- pL-CRISPR.SFFV.tRFP
- pLenti-Cas-Guide
- pLenti-Cas9
- pLenti-Cas9-IRES-Puro
- pLenti-Cas9-P2A-tGFP
- pLenti-EF1a-Cas9-IRES-Puro
- pLenti-Guide-Puro
- pLKO.1-puro U6 sgRNA BfuAI stuffer
- pLSC-5
- pLVX-hyg-sgRNA1 Vector (Linear)
- pLVX-puro-Cas9
- pLV hUbC-Cas9-T2A-GFP
- pLV hUbC-dCas9-T2A-GFP
- pLV hUbC-dCas9 VP64-T2A-GFP
- pLX-sgRNA
- pMB66
- pMB70
- pME-Cas9
- pME-Cas9-T2A-GFP
- pMJ806
- pMJ841
- pMJ920
- pMLM3705
- pMSCV-LTR-dCas9-p65AD-BFP
- pMSCV-LTR-dCas9-VP64-BFP
- pMZ374
- pNW3
- pRGE31
- pRGEB31
- pRGEB32
- pROSA26-Puro-DNR
- pRPR1_gRNA_handle_RPR1t
- pSECC
- pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)
- pSLQ1658-dCas9-EGFP
- pSpCas9(BB)-2A-GFP (PX458)
- pSpCas9(BB)-2A-Puro (PX459) V2.0
- pSpCas9n(BB)-2A-GFP (PX461)
- pSpCas9n(BB)-2A-Puro (PX462) V2.0
- pSpCas9n(BB) (PX460)
- pSpCas9 (PX165)
- pSPgRNA
- pSQT834
- pSQT1313
- pST1374-N-NLS-flag-linker-Cas9-D10A
- pST1374-N-NLS-flag-linker-Cas9-H840A
- pST1374-NLS-flag-linker-Cas9
- pT3TS-nCas9n
- pT7-Cas9
- pT7-Cas9-Nickase
- pT7-gRNA
- pT7-Guide-IVT
- pTDH3-dCas9
- pTDH3-dCas9-Mxi1
- pTOL-hCMV-TET3G-Hygro Transactivator
- pTPGI_dCas9_VP64
- pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B
- pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E4orf6
- pU6-BbsI-chiRNA
- PU6::klp-12_sgRNA
- pUC57-sgRNA expression vector
- pUC119-gRNA
- Puro-Cas9 donor
- pWJ66
- pWJ68
- pwtCas9-bacteria
- pX260
- pX330
- pX330A-1x2
- pX330S-2
- pX334
- pX335
- PX551
- PX552
- pX600
- pX601
- pX602
- PX851
- PX852
- PX853
- PX854
- PX855
- PX856
- pY010 (pcDNA3.1-hAsCpf1)
- pY016 (pcDNA3.1-hLbCpf1)
- sgRNA(MS2) cloning backbone
- SP-Cas9
- SP-dCas9-VPR
- VP12
The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.
