pT7-Cas9

In vitro transcription vector with the T7 promoter, for producing myc- and FLAG®-tagged S. pyogenes Cas9.

Sequence Author: OriGene

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: CRISPR Plasmids | More Plasmid Sets
No matches
PmeI (5277) SV40 NLS SV40 NLS CMV enhancer pT7-Cas9 9036 bp
PmeI  (5277)
1 site
G T T T A A A C C A A A T T T G
Cas9
1029 .. 5132  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
1029 .. 5132  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
SV40 NLS
5133 .. 5153  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
5133 .. 5153  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
5160 .. 5180  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
5160 .. 5180  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
Myc
5202 .. 5231  =  30 bp
10 amino acids  =  1.2 kDa
Product: Myc (human c-Myc oncogene) epitope tag
Myc
5202 .. 5231  =  30 bp
10 amino acids  =  1.2 kDa
Product: Myc (human c-Myc oncogene) epitope tag
FLAG
5250 .. 5273  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
FLAG
5250 .. 5273  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
NeoR/KanR
7287 .. 8081  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
7287 .. 8081  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
hGH poly(A) signal
5320 .. 5942  =  623 bp
human growth hormone polyadenylation signal
hGH poly(A) signal
5320 .. 5942  =  623 bp
human growth hormone polyadenylation signal
ori
6091 .. 6679  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
6091 .. 6679  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
8606 .. 25  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
8606 .. 25  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
343 .. 722  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
343 .. 722  =  380 bp
human cytomegalovirus immediate early enhancer
SV40 promoter
8116 .. 8473  =  358 bp
SV40 enhancer and early promoter
SV40 promoter
8116 .. 8473  =  358 bp
SV40 enhancer and early promoter
CMV promoter
723 .. 926  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
723 .. 926  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
AmpR promoter
8475 .. 8579  =  105 bp
AmpR promoter
8475 .. 8579  =  105 bp
HSV TK poly(A) signal
7008 .. 7055  =  48 bp
herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)
HSV TK poly(A) signal
7008 .. 7055  =  48 bp
herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)
T7 promoter
952 .. 970  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
952 .. 970  =  19 bp
promoter for bacteriophage T7 RNA polymerase
ORF:  882 .. 5279  =  4398 bp
ORF:  1465 amino acids  =  169.8 kDa
ORF:  6828 .. 7061  =  234 bp
ORF:  77 amino acids  =  8.6 kDa
ORF:  7236 .. 7772  =  537 bp
ORF:  178 amino acids  =  19.9 kDa
ORF:  6817 .. 7266  =  450 bp
ORF:  149 amino acids  =  16.3 kDa
ORF:  1287 .. 1529  =  243 bp
ORF:  80 amino acids  =  9.6 kDa
ORF:  3081 .. 3425  =  345 bp
ORF:  114 amino acids  =  13.5 kDa
ORF:  3579 .. 3983  =  405 bp
ORF:  134 amino acids  =  15.6 kDa
ORF:  4452 .. 5075  =  624 bp
ORF:  207 amino acids  =  24.3 kDa
ORF:  7287 .. 8081  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  5684 .. 5908  =  225 bp
ORF:  74 amino acids  =  8.1 kDa
ORF:  7523 .. 7909  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
Click here to try SnapGene

Download pT7-Cas9.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.