pTDH3-dCas9-Mxi1

Yeast centromeric vector for expressing catalytically inactive dCas9 fused to the Mxi1 transcriptional repressor domain.

Sequence Author: Qi

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ATG pTDH3-dCas9-Mxi1 11,211 bp
ATG
816 .. 818  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
816 .. 818  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
SV40 NLS
825 .. 845  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
825 .. 845  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
dCas9
852 .. 4952  =  4101 bp
1367 amino acids  =  158.2 kDa
5 segments
   Segment 1:  
   852 .. 875  =  24 bp
   8 amino acids  =  923.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
852 .. 4952  =  4101 bp
1367 amino acids  =  158.2 kDa
5 segments
   Segment 2:  
   876 .. 878  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
852 .. 4952  =  4101 bp
1367 amino acids  =  158.2 kDa
5 segments
   Segment 3:  
   879 .. 3365  =  2487 bp
   829 amino acids  =  96.3 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
852 .. 4952  =  4101 bp
1367 amino acids  =  158.2 kDa
5 segments
   Segment 4:  
   3366 .. 3368  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
852 .. 4952  =  4101 bp
1367 amino acids  =  158.2 kDa
5 segments
   Segment 5:  
   3369 .. 4952  =  1584 bp
   528 amino acids  =  60.9 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
852 .. 4952  =  4101 bp
1367 amino acids  =  158.2 kDa
5 segments
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
SV40 NLS
4962 .. 4982  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
4962 .. 4982  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
Mxi1
5016 .. 5138  =  123 bp
41 amino acids  =  4.9 kDa
Product: transcriptional repressor domain from the mouse Max-interacting protein 1 (Schreiber-Agus et al., 1995)
Mxi1
5016 .. 5138  =  123 bp
41 amino acids  =  4.9 kDa
Product: transcriptional repressor domain from the mouse Max-interacting protein 1 (Schreiber-Agus et al., 1995)
LEU2
8854 .. 9948  =  1095 bp
364 amino acids  =  39.0 kDa
Product: 3-isopropylmalate dehydrogenase, required for leucine biosynthesis
yeast auxotrophic marker
LEU2
8854 .. 9948  =  1095 bp
364 amino acids  =  39.0 kDa
Product: 3-isopropylmalate dehydrogenase, required for leucine biosynthesis
yeast auxotrophic marker
AmpR
6616 .. 7476  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   6616 .. 7407  =  792 bp
   263 amino acids  =  29.0 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6616 .. 7476  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   7408 .. 7476  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6616 .. 7476  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
GAP promoter
136 .. 802  =  667 bp
promoter for glyceraldehyde-3-phosphate dehydrogenase
GAP promoter
136 .. 802  =  667 bp
promoter for glyceraldehyde-3-phosphate dehydrogenase
ori
5857 .. 6445  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
5857 .. 6445  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CEN/ARS
7618 .. 8121  =  504 bp
S. cerevisiae CEN6 centromere fused to an autonomously replicating sequence
CEN/ARS
7618 .. 8121  =  504 bp
S. cerevisiae CEN6 centromere fused to an autonomously replicating sequence
f1 ori
10,656 .. 11,111  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
10,656 .. 11,111  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
LEU2 promoter
9949 .. 10,356  =  408 bp
LEU2 promoter
9949 .. 10,356  =  408 bp
ADH1 terminator
5151 .. 5338  =  188 bp
transcription terminator for alcohol dehydrogenase 1
ADH1 terminator
5151 .. 5338  =  188 bp
transcription terminator for alcohol dehydrogenase 1
AmpR promoter
7477 .. 7581  =  105 bp
AmpR promoter
7477 .. 7581  =  105 bp
ORF:  4699 .. 4983  =  285 bp
ORF:  94 amino acids  =  10.0 kDa
ORF:  5305 .. 5532  =  228 bp
ORF:  75 amino acids  =  8.2 kDa
ORF:  245 .. 514  =  270 bp
ORF:  89 amino acids  =  10.1 kDa
ORF:  6746 .. 7012  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  8975 .. 9286  =  312 bp
ORF:  103 amino acids  =  11.1 kDa
ORF:  816 .. 5144  =  4329 bp
ORF:  1442 amino acids  =  166.5 kDa
ORF:  6616 .. 7476  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  8854 .. 9948  =  1095 bp
ORF:  364 amino acids  =  39.0 kDa
ORF:  717 .. 971  =  255 bp
ORF:  84 amino acids  =  9.3 kDa
ORF:  2469 .. 2942  =  474 bp
ORF:  157 amino acids  =  17.4 kDa
ORF:  4254 .. 4640  =  387 bp
ORF:  128 amino acids  =  14.5 kDa
ORF:  2762 .. 3130  =  369 bp
ORF:  122 amino acids  =  14.1 kDa
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Download pTDH3-dCas9-Mxi1.dna file

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