pTPGI_dCas9_VP64

Yeast plasmid for inducible expression of catalytically inactive S. pyogenes dCas9 fused to the VP64 transcriptional activation domain.

Sequence Author: Lu Lab / Addgene #49013

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: CRISPR Plasmids | More Plasmid Sets
No matches
ATG pTPGI_dCas9_VP64 10,987 bp
ATG
4112 .. 4114  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
4112 .. 4114  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
SV40 NLS
4124 .. 4144  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
4124 .. 4144  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
dCas9
4154 .. 8254  =  4101 bp
1367 amino acids  =  158.2 kDa
5 segments
   Segment 1:  
   4154 .. 4177  =  24 bp
   8 amino acids  =  923.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
4154 .. 8254  =  4101 bp
1367 amino acids  =  158.2 kDa
5 segments
   Segment 2:  
   4178 .. 4180  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
4154 .. 8254  =  4101 bp
1367 amino acids  =  158.2 kDa
5 segments
   Segment 3:  
   4181 .. 6667  =  2487 bp
   829 amino acids  =  96.3 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
4154 .. 8254  =  4101 bp
1367 amino acids  =  158.2 kDa
5 segments
   Segment 4:  
   6668 .. 6670  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
4154 .. 8254  =  4101 bp
1367 amino acids  =  158.2 kDa
5 segments
   Segment 5:  
   6671 .. 8254  =  1584 bp
   528 amino acids  =  60.9 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
4154 .. 8254  =  4101 bp
1367 amino acids  =  158.2 kDa
5 segments
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
VP64
8291 .. 8440  =  150 bp
50 amino acids  =  5.5 kDa
Product: tetrameric repeat of the minimal activation domain of herpes simplex virus VP16 (Beerli et al., 1998)
VP64
8291 .. 8440  =  150 bp
50 amino acids  =  5.5 kDa
Product: tetrameric repeat of the minimal activation domain of herpes simplex virus VP16 (Beerli et al., 1998)
AmpR
9927 .. 10,787  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   9927 .. 10,718  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
9927 .. 10,787  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   10,719 .. 10,787  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
9927 .. 10,787  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
TRP1
468 .. 1142  =  675 bp
224 amino acids  =  24.1 kDa
Product: phosphoribosylanthranilate isomerase, required for tryptophan biosynthesis
yeast auxotrophic marker
TRP1
468 .. 1142  =  675 bp
224 amino acids  =  24.1 kDa
Product: phosphoribosylanthranilate isomerase, required for tryptophan biosynthesis
yeast auxotrophic marker
TetR
2088 .. 2711  =  624 bp
207 amino acids  =  23.4 kDa
Product: tetracycline repressor TetR
TetR binds to the tetracycline operator tetO to inhibit transcription. This inhibition can be relieved by adding tetracycline or doxycycline.
TetR
2088 .. 2711  =  624 bp
207 amino acids  =  23.4 kDa
Product: tetracycline repressor TetR
TetR binds to the tetracycline operator tetO to inhibit transcription. This inhibition can be relieved by adding tetracycline or doxycycline.
ori
9168 .. 9756  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
9168 .. 9756  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
1241 .. 1696  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
1241 .. 1696  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
TPGI promoter
3643 .. 4084  =  442 bp
S. cerevisiae GAL1 promoter modified to contain two copies of the tet operator (Ellis et al., 2009)
TPGI promoter
3643 .. 4084  =  442 bp
S. cerevisiae GAL1 promoter modified to contain two copies of the tet operator (Ellis et al., 2009)
TEF1 promoter
2729 .. 3127  =  399 bp
promoter for EF-1α
TEF1 promoter
2729 .. 3127  =  399 bp
promoter for EF-1α
TRP1 promoter
187 .. 467  =  281 bp
TRP1 promoter
187 .. 467  =  281 bp
CYC1 terminator
8456 .. 8703  =  248 bp
transcription terminator for CYC1
CYC1 terminator
8456 .. 8703  =  248 bp
transcription terminator for CYC1
AmpR promoter
10,788 .. 10,892  =  105 bp
AmpR promoter
10,788 .. 10,892  =  105 bp
UAS
3643 .. 3760  =  118 bp
upstream activating sequence mediating Gal4-dependent induction
UAS
3643 .. 3760  =  118 bp
upstream activating sequence mediating Gal4-dependent induction
tet operator
3968 .. 3986  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
3968 .. 3986  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
3989 .. 4007  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
3989 .. 4007  =  19 bp
bacterial operator O2 for the tetR and tetA genes
ORF:  718 .. 999  =  282 bp
ORF:  93 amino acids  =  10.8 kDa
ORF:  10,057 .. 10,323  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  4112 .. 8446  =  4335 bp
ORF:  1444 amino acids  =  166.4 kDa
ORF:  468 .. 1142  =  675 bp
ORF:  224 amino acids  =  24.1 kDa
ORF:  3159 .. 3650  =  492 bp
ORF:  163 amino acids  =  17.6 kDa
ORF:  2993 .. 3463  =  471 bp
ORF:  156 amino acids  =  17.3 kDa
ORF:  4039 .. 4278  =  240 bp
ORF:  79 amino acids  =  8.8 kDa
ORF:  1479 .. 1790  =  312 bp
ORF:  103 amino acids  =  11.3 kDa
ORF:  2088 .. 2711  =  624 bp
ORF:  207 amino acids  =  23.4 kDa
ORF:  8223 .. 8459  =  237 bp
ORF:  78 amino acids  =  9.5 kDa
ORF:  9927 .. 10,787  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
Click here to try SnapGene

Download pTPGI_dCas9_VP64.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.