pUC119-gRNA

CRISPR plasmid for expressing a single guide RNA (sgRNA) in plant cells. Use together with HBT-pcoCas9.

Sequence Author: Sheen Lab / Addgene #52255

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: CRISPR Plasmids | More Plasmid Sets
No matches
polIII terminator pUC119-gRNA 3771 bp
AmpR
1971 .. 2831  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   1971 .. 2039  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1971 .. 2831  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   2040 .. 2831  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1971 .. 2831  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
3002 .. 3590  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
3002 .. 3590  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
1129 .. 1584  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
1129 .. 1584  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
AtU6-1 promoter
407 .. 710  =  304 bp
promoter for the Arabidopsis thaliana U6-1 snRNA gene (Waibel and Filipowicz, 1990)
AtU6-1 promoter
407 .. 710  =  304 bp
promoter for the Arabidopsis thaliana U6-1 snRNA gene (Waibel and Filipowicz, 1990)
AmpR promoter
1866 .. 1970  =  105 bp
AmpR promoter
1866 .. 1970  =  105 bp
gRNA scaffold
731 .. 806  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
gRNA scaffold
731 .. 806  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
polIII terminator
807 .. 812  =  6 bp
RNA polymerase III transcription terminator
polIII terminator
807 .. 812  =  6 bp
RNA polymerase III transcription terminator
ORF:  874 .. 1152  =  279 bp
ORF:  92 amino acids  =  10.8 kDa
ORF:  1971 .. 2831  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  870 .. 1109  =  240 bp
ORF:  79 amino acids  =  9.3 kDa
ORF:  488 .. 727  =  240 bp
ORF:  79 amino acids  =  8.8 kDa
ORF:  2435 .. 2701  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
Click here to try SnapGene

Download pUC119-gRNA.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.