pUC57-sgRNA expression vector

Plasmid for in vitro transcription of a single guide RNA (sgRNA) using the T7 promoter.

Sequence Author: Huang Lab / Addgene #51132

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M13 fwd BsaI (456) BsaI (481) polIII terminator pUC57-sgRNA expression vector 2792 bp
BsaI  (456)
2 sites
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BsaI  (481)
2 sites
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
KanR
1777 .. 2592  =  816 bp
271 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes
KanR
1777 .. 2592  =  816 bp
271 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes
ori
1018 .. 1606  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1018 .. 1606  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
AmpR promoter
2593 .. 2697  =  105 bp
AmpR promoter
2593 .. 2697  =  105 bp
gRNA scaffold
482 .. 557  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
gRNA scaffold
482 .. 557  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
T7 promoter
442 .. 460  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
442 .. 460  =  19 bp
promoter for bacteriophage T7 RNA polymerase
M13 fwd
379 .. 395  =  17 bp
common sequencing primer, one of multiple similar variants
M13 fwd
379 .. 395  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
615 .. 631  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
615 .. 631  =  17 bp
common sequencing primer, one of multiple similar variants
polIII terminator
558 .. 563  =  6 bp
RNA polymerase III transcription terminator
polIII terminator
558 .. 563  =  6 bp
RNA polymerase III transcription terminator
ORF:  186 .. 488  =  303 bp
ORF:  100 amino acids  =  11.6 kDa
ORF:  1755 .. 1982  =  228 bp
ORF:  75 amino acids  =  8.8 kDa
ORF:  1777 .. 2592  =  816 bp
ORF:  271 amino acids  =  31.0 kDa
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Download pUC57-sgRNA expression vector.dna file

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