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Zhang lab vector for expressing a Cas9 nickase mutant, a tracrRNA, and a guide sequence. Also known as pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro.


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Map and Sequence File:    Download    Open    

Sequence Author:  Zhang Lab / Addgene #42333

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