pY010 (pcDNA3.1-hAsCpf1)

Zhang lab mammalian expression vector encoding a humanized version of the Acidaminococcus CRISPR-associated protein Cpf1.

Sequence Author: Zhang Lab / Addgene #69982

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nucleoplasmin NLS CMV enhancer pY010 (pcDNA3.1-hAsCpf1) 9464 bp
AsCpf1
929 .. 4849  =  3921 bp
1307 amino acids  =  151.2 kDa
Product: CRISPR-associated protein from Acidaminococcus sp. BV3L6 (Zetsche et al., 2015)
AsCpf1
929 .. 4849  =  3921 bp
1307 amino acids  =  151.2 kDa
Product: CRISPR-associated protein from Acidaminococcus sp. BV3L6 (Zetsche et al., 2015)
nucleoplasmin NLS
4850 .. 4897  =  48 bp
16 amino acids  =  1.7 kDa
Product: bipartite nuclear localization signal from nucleoplasmin
nucleoplasmin NLS
4850 .. 4897  =  48 bp
16 amino acids  =  1.7 kDa
Product: bipartite nuclear localization signal from nucleoplasmin
3xHA
4904 .. 4984  =  81 bp
27 amino acids  =  3.3 kDa
Product: three tandem HA epitope tags
3xHA
4904 .. 4984  =  81 bp
27 amino acids  =  3.3 kDa
Product: three tandem HA epitope tags
AmpR
8468 .. 9328  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   8468 .. 9259  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
8468 .. 9328  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   9260 .. 9328  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
8468 .. 9328  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
NeoR/KanR
6172 .. 6966  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
6172 .. 6966  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
ori
7712 .. 8297  =  586 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
7712 .. 8297  =  586 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
5334 .. 5762  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
5334 .. 5762  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
235 .. 614  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
235 .. 614  =  380 bp
human cytomegalovirus immediate early enhancer
SV40 promoter
5776 .. 6105  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
5776 .. 6105  =  330 bp
SV40 enhancer and early promoter
bGH poly(A) signal
5064 .. 5288  =  225 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
5064 .. 5288  =  225 bp
bovine growth hormone polyadenylation signal
CMV promoter
615 .. 818  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
615 .. 818  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal
7140 .. 7261  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
7140 .. 7261  =  122 bp
SV40 polyadenylation signal
AmpR promoter
9329 .. 9433  =  105 bp
AmpR promoter
9329 .. 9433  =  105 bp
T7 promoter
863 .. 881  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
863 .. 881  =  19 bp
promoter for bacteriophage T7 RNA polymerase
SV40 ori
5956 .. 6091  =  136 bp
SV40 origin of replication
SV40 ori
5956 .. 6091  =  136 bp
SV40 origin of replication
ORF:  6172 .. 6966  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  929 .. 4987  =  4059 bp
ORF:  1352 amino acids  =  156.3 kDa
ORF:  5285 .. 5551  =  267 bp
ORF:  88 amino acids  =  9.3 kDa
ORF:  6344 .. 6730  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
ORF:  8598 .. 8864  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  4638 .. 4964  =  327 bp
ORF:  108 amino acids  =  12.0 kDa
ORF:  8468 .. 9328  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  1663 .. 2199  =  537 bp
ORF:  178 amino acids  =  20.5 kDa
ORF:  6481 .. 7017  =  537 bp
ORF:  178 amino acids  =  19.8 kDa
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Download pY010 (pcDNA3.1-hAsCpf1).dna file

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