pcDNA3.1-CibN-dCas9-CibN

Plasmid encoding two copies of CIBN fused to catalytically inactive dCas9, for use in the light-activated CRISPR-Cas9 effector (LACE) system.

Sequence Author: Gersbach Lab / Addgene #60553

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: CRISPR Plasmids | More Plasmid Sets
No matches
SV40 NLS CMV enhancer pcDNA3.1-CibN-dCas9-CibN 10,632 bp
ATG
5013 .. 5015  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
5013 .. 5015  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
CIBN
5064 .. 5570  =  507 bp
169 amino acids  =  18.9 kDa
Product: N-terminal portion of the CIB1 transcription factor from Arabidopsis thaliana
binds to CRY2 (cryptochrome 2) that has absorbed blue light (Kennedy et al., 2010)
CIBN
5064 .. 5570  =  507 bp
169 amino acids  =  18.9 kDa
Product: N-terminal portion of the CIB1 transcription factor from Arabidopsis thaliana
binds to CRY2 (cryptochrome 2) that has absorbed blue light (Kennedy et al., 2010)
FLAG
5577 .. 5600  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
FLAG
5577 .. 5600  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
SV40 NLS
5607 .. 5627  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
5607 .. 5627  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
dCas9
5637 .. 9740  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 1:  
   5637 .. 5663  =  27 bp
   9 amino acids  =  1.1 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
5637 .. 9740  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 2:  
   5664 .. 5666  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
5637 .. 9740  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 3:  
   5667 .. 8153  =  2487 bp
   829 amino acids  =  96.3 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
5637 .. 9740  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 4:  
   8154 .. 8156  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
5637 .. 9740  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 5:  
   8157 .. 9740  =  1584 bp
   528 amino acids  =  60.9 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
5637 .. 9740  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
SV40 NLS
9765 .. 9785  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
9765 .. 9785  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
CIBN
9798 .. 10,307  =  510 bp
170 amino acids  =  19.1 kDa
Product: N-terminal portion of the CIB1 transcription factor from Arabidopsis thaliana
binds to CRY2 (cryptochrome 2) that has absorbed blue light (Kennedy et al., 2010)
CIBN
9798 .. 10,307  =  510 bp
170 amino acids  =  19.1 kDa
Product: N-terminal portion of the CIB1 transcription factor from Arabidopsis thaliana
binds to CRY2 (cryptochrome 2) that has absorbed blue light (Kennedy et al., 2010)
AmpR
3113 .. 3973  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   3113 .. 3904  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3113 .. 3973  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   3905 .. 3973  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3113 .. 3973  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
NeoR/KanR
817 .. 1611  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
817 .. 1611  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
ori
2357 .. 2942  =  586 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
2357 .. 2942  =  586 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
10,611 .. 407  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
10,611 .. 407  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
4344 .. 4723  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
4344 .. 4723  =  380 bp
human cytomegalovirus immediate early enhancer
SV40 promoter
421 .. 750  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
421 .. 750  =  330 bp
SV40 enhancer and early promoter
bGH poly(A) signal
10,341 .. 10,565  =  225 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
10,341 .. 10,565  =  225 bp
bovine growth hormone polyadenylation signal
CMV promoter
4724 .. 4927  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
4724 .. 4927  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal
1785 .. 1906  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1785 .. 1906  =  122 bp
SV40 polyadenylation signal
AmpR promoter
3974 .. 4078  =  105 bp
AmpR promoter
3974 .. 4078  =  105 bp
SV40 ori
601 .. 736  =  136 bp
SV40 origin of replication
SV40 ori
601 .. 736  =  136 bp
SV40 origin of replication
ORF:  817 .. 1611  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  989 .. 1375  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
ORF:  10,562 .. 196  =  267 bp
ORF:  88 amino acids  =  9.3 kDa
ORF:  3243 .. 3509  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  5013 .. 10,310  =  5298 bp
ORF:  1765 amino acids  =  202.9 kDa
ORF:  1126 .. 1662  =  537 bp
ORF:  178 amino acids  =  19.8 kDa
ORF:  3113 .. 3973  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  5222 .. 5476  =  255 bp
ORF:  84 amino acids  =  9.4 kDa
ORF:  9959 .. 10,213  =  255 bp
ORF:  84 amino acids  =  9.4 kDa
Click here to try SnapGene

Download pcDNA3.1-CibN-dCas9-CibN.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.