pACYCDuet-1
Bacterial vector with a p15A origin for the co-expression of two genes.
Sequence Author: MilliporeSigma (Novagen)
Explore Over 2.7k Plasmids: pET & Duet Vectors (Novagen) | More Plasmid Sets
No matches
|
| ||
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
|
| ||
The 1-base overhangs produced by EcoNI may be hard to ligate.Sticky ends from different EcoNI sites may not be compatible. |
| ||
Sticky ends from different BstAPI sites may not be compatible. |
|
| ||
Sticky ends from different AflIII sites may not be compatible. |
|
| ||
* Blocked by Dam methylation. BclI is typically used at 50-55°C, but is 50% active at 37°C. |
| ||
Sticky ends from different BstEII sites may not be compatible.BstEII is typically used at 60°C, but is 50% active at 37°C. |
| ||
ApaI can be used between 25°C and 37°C. |
|
| ||
The 1-base overhangs produced by BciVI may be hard to ligate.Sticky ends from different BciVI sites may not be compatible. |
|
| ||
Efficient cleavage requires at least two copies of the PluTI recognition sequence. |
|
| ||
* Blocked by Dcm methylation. Efficient cleavage requires at least two copies of the NarI recognition sequence. |
|
| ||
Cleavage may be enhanced when more than one copy of the AcuI recognition sequence is present. Sticky ends from different AcuI sites may not be compatible.After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.For full activity, add fresh S-adenosylmethionine (SAM). |
|
|
|
|
|
|
| ||
Efficient cleavage requires at least two copies of the BfuAI recognition sequence. Sticky ends from different BfuAI sites may not be compatible.BfuAI is typically used at 50°C, but is 50% active at 37°C. |
| ||
Efficient cleavage requires at least two copies of the BspMI recognition sequence. Sticky ends from different BspMI sites may not be compatible. |
|
|
|
|
|
|
|
| ||
BsrGI is typically used at 37°C, but is even more active at 60°C. |
| ||
Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
|
|
| ||
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
| ||
Efficient cleavage requires at least two copies of the NgoMIV recognition sequence. |
| ||
Efficient cleavage requires at least two copies of the NaeI recognition sequence. |
| ||
FseI gradually loses activity when stored at -20°C. |
|
|
|
|
|
|
| ||
Sticky ends from different AvaI sites may not be compatible. |
| ||
Sticky ends from different BsoBI sites may not be compatible.BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C. |
| ||
PaeR7I does not recognize the sequence CTCTCGAG. |
|
|
|
|
| ||
Sticky ends from different BlpI sites may not be compatible. |
| ||
Sticky ends from different EcoO109I sites may not be compatible. |
| ||
Sticky ends from different Bsu36I sites may not be compatible. |
| ||
Sticky ends from different DrdI sites may not be compatible. |
| ||
The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible. |
| ||
The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible. |
|
|
|
| ||
Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present. This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.Sticky ends from different Bpu10I sites may not be compatible. |
|
|
|
|
|
|
| ||
Efficient cleavage requires at least two copies of the SgrAI recognition sequence. |
| ||
Efficient cleavage requires at least two copies of the SacII recognition sequence. |
ACYCDuetUP1 Primer 19-mer / 63% GC 1 binding site 3949 .. 3967 = 19 annealed bases Tm = 60°C |
DuetUP2 Primer 20-mer / 50% GC 1 binding site 189 .. 208 = 20 annealed bases Tm = 57°C |
DuetDOWN1 Primer 20-mer / 50% GC 1 binding site 189 .. 208 = 20 annealed bases Tm = 57°C |
T7 Terminator Primer 19-mer / 53% GC 1 binding site 448 .. 466 = 19 annealed bases Tm = 57°C |
lacI 2786 .. 3868 = 1083 bp 360 amino acids = 38.6 kDa Product: lac repressor The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
lacI 2786 .. 3868 = 1083 bp 360 amino acids = 38.6 kDa Product: lac repressor The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
CmR 729 .. 1388 = 660 bp 219 amino acids = 25.7 kDa Product: chloramphenicol acetyltransferase confers resistance to chloramphenicol |
CmR 729 .. 1388 = 660 bp 219 amino acids = 25.7 kDa Product: chloramphenicol acetyltransferase confers resistance to chloramphenicol |
p15A ori 2017 .. 2561 = 545 bp Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin. |
p15A ori 2017 .. 2561 = 545 bp Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin. |
MCS-2 297 .. 438 = 142 bp multiple cloning site 2 |
MCS-2 297 .. 438 = 142 bp multiple cloning site 2 |
cat promoter 1389 .. 1491 = 103 bp promoter of the E. coli cat gene |
cat promoter 1389 .. 1491 = 103 bp promoter of the E. coli cat gene |
MCS-1 69 .. 168 = 100 bp multiple cloning site 1 |
MCS-1 69 .. 168 = 100 bp multiple cloning site 1 |
lacI promoter 3869 .. 3946 = 78 bp |
lacI promoter 3869 .. 3946 = 78 bp |
T7 terminator 462 .. 509 = 48 bp transcription terminator for bacteriophage T7 RNA polymerase |
T7 terminator 462 .. 509 = 48 bp transcription terminator for bacteriophage T7 RNA polymerase |
lac operator 233 .. 257 = 25 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
lac operator 233 .. 257 = 25 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
lac operator 3 .. 27 = 25 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
lac operator 3 .. 27 = 25 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
T7 promoter 3992 .. 2 = 19 bp promoter for bacteriophage T7 RNA polymerase |
T7 promoter 3992 .. 2 = 19 bp promoter for bacteriophage T7 RNA polymerase |
T7 promoter 214 .. 232 = 19 bp promoter for bacteriophage T7 RNA polymerase |
T7 promoter 214 .. 232 = 19 bp promoter for bacteriophage T7 RNA polymerase |
RBS 58 .. 63 = 6 bp ribosome binding site |
RBS 58 .. 63 = 6 bp ribosome binding site |
RBS 286 .. 291 = 6 bp ribosome binding site |
RBS 286 .. 291 = 6 bp ribosome binding site |
ORF: 2752 .. 3003 = 252 bp ORF: 83 amino acids = 9.1 kDa |
ORF: 1932 .. 2210 = 279 bp ORF: 92 amino acids = 10.4 kDa |
ORF: 3618 .. 158 = 549 bp ORF: 182 amino acids = 19.6 kDa |
ORF: 729 .. 1388 = 660 bp ORF: 219 amino acids = 25.7 kDa |
ORF: 1899 .. 2171 = 273 bp ORF: 90 amino acids = 9.7 kDa |
ORF: 2766 .. 3029 = 264 bp ORF: 87 amino acids = 8.9 kDa |
ORF: 2786 .. 3745 = 960 bp ORF: 319 amino acids = 34.1 kDa |
Click here to try SnapGene |
Download pACYCDuet-1.dna file
SnapGene
SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures
- Fast accurate construct design for all major molecular cloning techniques
- Validate sequenced constructs using powerful alignment tools
- Customize plasmid maps with flexible annotation and visualization controls
- Automatically generate a rich graphical history of every edit and procedure
SnapGene Viewer
SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.
- Gain unparalleled visibility of your plasmids, DNA and protein sequences
- Annotate features on your plasmids using the curated feature database
- Store, search, and share your sequences, files and maps