Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI (7205) 1 site
Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BmgBI (6995) 1 site
This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
SmaI (6961) 1 site
SmaI can be used at 37°C for brief incubations.
SrfI (6961) 1 site
AvaI (6959) 1 site
Sticky ends from different AvaI sites may not be compatible.
BsoBI (6959) 1 site
Sticky ends from different BsoBI sites may not be compatible.BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
TspMI (6959) 1 site
XmaI (6959) 1 site
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
BbvCI (6815) 1 site
PasI (6322) 1 site
Sticky ends from different PasI sites may not be compatible.
EcoRI (6090) 1 site
EagI (5770) 1 site
NotI (5770) 1 site
PmlI (5632) 1 site
SwaI (5384) 1 site
SwaI is typically used at 25°C, but is 50% active at 37°C.
