pCOLA-3-DEST
Gateway® Nova bacterial destination vector with a ColA origin for expressing proteins tagged at the N-terminus with Strep-Tag II.
Sequence Author: MilliporeSigma (Novagen)
Explore Over 2.7k Plasmids: pET & Duet Vectors (Novagen) | More Plasmid Sets
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Sticky ends from different EcoO109I sites may not be compatible. |
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Sticky ends from different BlpI sites may not be compatible. |
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Sticky ends from different DraIII sites may not be compatible. |
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Efficient cleavage requires at least two copies of the BfuAI recognition sequence. Sticky ends from different BfuAI sites may not be compatible.BfuAI is typically used at 50°C, but is 50% active at 37°C. |
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Efficient cleavage requires at least two copies of the BspMI recognition sequence. Sticky ends from different BspMI sites may not be compatible. |
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| * Blocked by Dam methylation. |
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Sticky ends from different AlwNI sites may not be compatible. |
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Sticky ends from different PasI sites may not be compatible. |
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Sticky ends from different BtgI sites may not be compatible. |
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Sticky ends from different PfoI sites may not be compatible. |
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Sticky ends from different BstAPI sites may not be compatible. |
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| * Blocked by Dam methylation. BclI is typically used at 50-55°C, but is 50% active at 37°C. |
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Sticky ends from different BstEII sites may not be compatible.BstEII is typically used at 60°C, but is 50% active at 37°C. |
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Sticky ends from different Bsu36I sites may not be compatible. |
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Sticky ends from different DrdI sites may not be compatible. |
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